Thbs1 (NM_011580) Mouse Tagged ORF Clone

CAT#: MR211744

Thbs1 (Myc-DDK-tagged) - Mouse thrombospondin 1 (Thbs1)

Product Images

Circular map for MR211744

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Thbs1
• Synonyms: tbs; tbsp1; Thbs-; Thbs-1; TS; TSP; TSP-1; TSP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211744 representing NM_011580
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTCCTGCGGGGACTAGGTGTCCTGTTCCTGTTGCATATGTGTGGAAGCAACCGCATTCCAGAGT
  CTGGGGGAGATAACGGTGTGTTTGACATCTTTGAACTCATTGGAGGTGCACGAAGGGGCCCCGGTCGCCG
  ACTGGTGAAGGGCCAAGATCTATCCAGCCCCGCCTTCCGCATTGAGAATGCCAACCTGATCCCCGCTGTG
  CCGGATGACAAGTTCCAAGACCTACTGGACGCTGTGTGGGCCGACAAAGGCTTCATCTTCCTGGCTTCCT
  TGAGGCAGATGAAGAAGACCCGGGGCACACTCCTGGCTGTGGAACGGAAAGACAACACTGGCCAGATCTT
  CAGTGTGGTCTCCAACGGCAAAGCTGGCACCCTGGACCTGAGCCTGAGCCTGCCAGGGAAGCAACAAGTG
  GTGTCAGTGGAGGAAGCTCTCCTGGCCACTGGCCAGTGGAAGAGCATCACGCTGTTTGTTCAAGAGGACC
  GGGCTCAACTCTACATAGACTGTGATAAGATGGAGAGCGCGGAGCTGGATGTACCCATCCAGAGCATCTT
  CACCAGGGATCTGGCCAGCGTTGCCAGGCTCCGAGTTGCAAAGGGAGATGTCAATGACAATTTTCAGGGG
  GTGCTGCAGAATGTGAGGTTTGTCTTTGGAACCACCCCAGAAGACATTCTCAGGAACAAAGGCTGCTCCA
  GCTCAGCTACCAACGTCCTTCTTACCCTTGACAACAACGTGGTGAACGGTTCCAGCCCTGCTATCCGCAC
  CAACTACATCGGCCACAAAACAAAGGACCTCCAAGCTATCTGTGGCCTCTCCTGTGATGAACTATCCAGC
  ATGGTCCTGGAACTGAAGGGCCTGCGCACCATCGTGACCACTCTGCAGGACAGCATCCGAAAAGTGACGG
  AAGAGAACAGAGAGCTGGTCAGTGAGCTGAAGCGGCCTCCCCTCTGCTTTCACAATGGAGTCCAGTACAA
  GAACAACGAGGAGTGGACTGTAGACAGTTGCACAGAGTGTCACTGCCAGAACTCGGTTACCATCTGCAAA
  AAGGTGTCCTGTCCCATCATGCCCTGCTCCAACGCCACAGTTCCTGATGGTGAATGCTGCCCACGGTGCT
  GGCCCAGCGACTCTGCTGACGATGGCTGGTCTCCCTGGTCTGAGTGGACCTCCTGCTCTGCCACATGTGG
  CAATGGAATTCAGCAACGTGGTCGTTCCTGTGACAGCCTCAACAACAGATGCGAGGGCTCTTCGGTACAG
  ACAAGGACCTGCCACATTCAGGAGTGTGACAAAAGATTTAAACAGGATGGTGGCTGGAGTCACTGGTCTC
  CATGGTCGTCCTGTTCTGTGACCTGTGGTGACGGTGTGATCACAAGGATCCGGCTCTGCAACTCCCCCAG
  CCCCCAGATGAACGGGAAGCCCTGTGAAGGTGAAGCCCGGGAGACCAAAGCCTGCAAGAAAGACGCCTGC
  CCAATTAATGGAGGCTGGGGTCCCTGGTCACCATGGGACATCTGCTCTGTCACCTGTGGAGGAGGAGTGC
  AGAGACGCAGCCGACTCTGTAACAACCCCACACCCCAGTTTGGAGGCAAAGACTGTGTTGGCGATGTGAC
  AGAAAATCAAGTTTGCAACAAGCAGGACTGCCCAATTGATGGATGCCTGTCCAATCCCTGCTTTGCTGGT
  GCCAAGTGTACTAGCTACCCTGATGGTAGCTGGAAATGTGGTGCGTGTCCTCCTGGCTACAGTGGAAATG
  GCATCCAGTGCAAAGACGTCGATGAGTGCAAAGAAGTGCCTGATGCTTGCTTCAATCACAACGGAGAACA
  TCGGTGCAAGAACACAGATCCTGGCTACAACTGCCTGCCCTGCCCACCACGATTCACTGGCTCACAGCCC
  TTCGGCCGAGGTGTCGAACATGCCATGGCCAACAAACAGGTGTGCAAACCGCGAAACCCCTGCACGGACG
  GGACGCATGACTGCAACAAGAACGCTAAGTGCAACTACCTGGGTCACTACAGTGACCCCATGTACCGCTG
  TGAGTGCAAGCCCGGCTATGCAGGCAATGGCATCATCTGCGGAGAGGACACAGACCTGGACGGCTGGCCT
  AATGAAAACCTGGTGTGTGTGGCCAACGCAACCTACCACTGCAAAAAGGACAACTGCCCCAACCTTCCCA
  ACTCGGGGCAGGAAGACTATGACAAGGACGGGATTGGCGATGCCTGCGATGATGACGATGACAACGACAA
  GATCCCCGATGACAGGGACAACTGTCCATTCCATTACAACCCAGCCCAGTATGACTATGACAGAGATGAT
  GTGGGAGACCGCTGTGACAACTGCCCCTACAACCACAACCCTGACCAAGCAGACACAGACAAAAACGGGG
  AGGGCGATGCCTGTGCTGTGGACATCGATGGAGATGGAATCCTCAATGAACGAGACAACTGCCAGTACGT
  TTACAACGTGGACCAGAGGGACACGGACATGGATGGGGTTGGAGATCAGTGTGACAACTGCCCCCTGGAA
  CACAATCCAGACCAGCTGGACTCTGACTCAGACCTCATAGGGGACACTTGTGACAACAATCAGGACATCG
  ATGAGGATGGCCATCAGAACAATCTGGACAACTGTCCCTATGTGCCTAATGCCAACCAGGCCGACCATGA
  TAAAGATGGCAAAGGAGATGCCTGTGACCATGACGATGACAATGACGGCATCCCTGATGACAGAGACAAC
  TGCAGGCTGGTGCCCAATCCTGACCAGAAGGACTCTGATGGTGATGGCCGAGGTGACGCCTGCAAAGACG
  ACTTTGACCATGACAATGTGCCAGATATTGATGACATCTGTCCTGAGAATTTTGACATCAGTGAAACCGA
  TTTCCGACAATTCCAGATGATTCCTCTAGATCCCAAAGGAACCTCCCAAAATGACCCTAACTGGGTTGTC
  CGCCATCAGGGCAAAGAACTTGTCCAGACTGTAAACTGTGACCCTGGACTTGCTGTAGGTTATGATGAGT
  TTAATGCTGTGGACTTCAGCGGTACCTTCTTCATCAACACCGAGAGAGATGATGACTACGCTGGCTTTGT
  TTTCGGCTACCAGTCCAGCAGCCGCTTCTACGTTGTGATGTGGAAACAAGTCACCCAGTCCTACTGGGAC
  ACCAACCCCACAAGGGCTCAGGGATACTCAGGCCTGTCTGTAAAGGTTGTGAACTCCACCACCGGCCCTG
  GCGAGCACCTGCGGAATGCACTGTGGCACACAGGAAACACCCCTGGCCAGGTGCGCACCCTGTGGCATGA
  CCCTCGCCACATCGGCTGGAAAGATTTCACTGCATACAGATGGCGTCTCAGCCACAGGCCAAAGACCGGT
  TATATCAGAGTGGTGATGTATGAAGGAAAGAAAATCATGGCTGACTCGGGACCCATCTATGACAAAACCT
  ACGCCGGCGGTAGACTAGGCCTGTTCGTCTTCTCTCAGGAAATGGTGTTCTTCTCAGACATGAAATACGA
  GTGTCGAGATTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211744 representing NM_011580
  Red=Cloning site Green=Tags(s)
  
  MELLRGLGVLFLLHMCGSNRIPESGGDNGVFDIFELIGGARRGPGRRLVKGQDLSSPAFRIENANLIPAV
  PDDKFQDLLDAVWADKGFIFLASLRQMKKTRGTLLAVERKDNTGQIFSVVSNGKAGTLDLSLSLPGKQQV
  VSVEEALLATGQWKSITLFVQEDRAQLYIDCDKMESAELDVPIQSIFTRDLASVARLRVAKGDVNDNFQG
  VLQNVRFVFGTTPEDILRNKGCSSSATNVLLTLDNNVVNGSSPAIRTNYIGHKTKDLQAICGLSCDELSS
  MVLELKGLRTIVTTLQDSIRKVTEENRELVSELKRPPLCFHNGVQYKNNEEWTVDSCTECHCQNSVTICK
  KVSCPIMPCSNATVPDGECCPRCWPSDSADDGWSPWSEWTSCSATCGNGIQQRGRSCDSLNNRCEGSSVQ
  TRTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGEARETKACKKDAC
  PINGGWGPWSPWDICSVTCGGGVQRRSRLCNNPTPQFGGKDCVGDVTENQVCNKQDCPIDGCLSNPCFAG
  AKCTSYPDGSWKCGACPPGYSGNGIQCKDVDECKEVPDACFNHNGEHRCKNTDPGYNCLPCPPRFTGSQP
  FGRGVEHAMANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWP
  NENLVCVANATYHCKKDNCPNLPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDD
  VGDRCDNCPYNHNPDQADTDKNGEGDACAVDIDGDGILNERDNCQYVYNVDQRDTDMDGVGDQCDNCPLE
  HNPDQLDSDSDLIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDRDN
  CRLVPNPDQKDSDGDGRGDACKDDFDHDNVPDIDDICPENFDISETDFRQFQMIPLDPKGTSQNDPNWVV
  RHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQVTQSYWD
  TNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTG
  YIRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDMKYECRDS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011580
• ORF Size: 3513 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011580.4
• RefSeq Size: 5802 bp
• RefSeq ORF: 3516 bp
• Locus ID: 21825
• Cytogenetics: 2 59.34 cM
• MW: 130.1 kDa
• Gene Summary: The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]