Kdm4b (NM_172132) Mouse Tagged ORF Clone

CAT#: MR211609

Kdm4b (Myc-DDK-tagged) - Mouse lysine (K)-specific demethylase 4B (Kdm4b)

Product Images

Circular map for MR211609

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kdm4b
• Synonyms: 4732474L06Rik; Jmjd2b; mKIAA0876
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211609 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGTCCGAGGACCACAGCGCCCAGAACCCCAGCTGTAAGATCATGACTTTCCGCCCCACCATGGATG
  AATTCAGGGACTTCAACAGATATGTGGCGTACATTGAGTCGCAGGGTGCCCACCGTGCTGGCCTGGCCAA
  GATCATTCCACCCAAGGAGTGGAAGCCACGGCAGACGTATGATGACATCGACGATGTGGTCATCCCAGCG
  CCTATTCAGCAGGTGGTGACGGGCCAGTCGGGGCTTTTCACACAGTACAACATCCAGAAGAAGGCCATGA
  CTGTGGGGGAGTACCGCCGCCTAGCCAATAGTGAAAAGTACTGTACCCCACGCCATCAGGACTTCGATGA
  CTTGGAGCGCAAGTACTGGAAGAACCTGACCTTTGTCTCACCCATCTATGGAGCTGACATCAGTGGCTCC
  CTGTACGACGATGACGTGGCGCAGTGGAACATCGGCAATCTGAGGACCATCCTAGACATGGTGGAGCGCG
  AATGCGGGACCATCATTGAGGGCGTGAATACACCCTACTTGTACTTTGGCATGTGGAAGACCACGTTTGC
  CTGGCACACAGAGGACATGGACCTCTACAGCATCAACTACCTGCACTTCGGCGAGCCTAAGTCCTGGTAC
  GCCATACCACCGGAGCACGGCAAGCGCCTGGAGCGCCTGGCCATAGGCTTCTTCCCTGGGAGCTCACAGG
  GCTGTGATGCCTTCCTAAGGCACAAGATGACACTCATCTCGCCCATCATCCTGAAGAAGTACGGCATCCC
  CTTCAGCCGGATTACACAGGAAGCTGGGGAGTTCATGATCACGTTCCCCTATGGTTACCATGCGGGCTTC
  AACCATGGCTTTAACTGCGCTGAGTCCACTAACTTCGCCACACTGCGCTGGATCGACTATGGCAAAGTGG
  CCACACAGTGTACATGTCGAAAGGACATGGTCAAGATCTCCATGGACGTGTTTGTACGCATCCTGCAGCC
  CGAACGCTATGAGCAATGGAAGCAAGGCCGTGACCTCACAGTGCTGGACCACACGAGGCCCACAGCCCTG
  AGCAGTCCTGAGCTCAGCTCCTGGAGTGCCTCGCGCACCTCCATCAAGGCCAAGCTCCTGCGCAGACAAA
  TTAGTGTGAAGGAGAGCAGACCCTGGAGAAAGGCTGAGGAGGAGAGGAGGCGGGAGCCGACCAGGAGGCC
  AGGGCCGGCGTCACACCGGAGACGGAGCCAGCCCAAGAAGTCAAAACCTGAAGAGTCCAGGTCTCCAGGG
  GAAGCCACGGCTGGGGTATCTACTCTGGATGAGGCCAGGGGCTGCTCCCGAGGAGAGGCCATGCCTGAGG
  ACGAGGAGGAGGAGGAGCTGCTACCTTCACAGGGCCATGAGGCCGAGGGTGTGGAAGAGGATGGCAGAGG
  CAAGCCTCGGCCTACTAAGGCCAGAAATAAGAAGAAGACTCCCAGCCCGTCGTCACCCCCGTTGCTGTCT
  GCCCCACCAGCCCTATTCCCCACCGAGGAGGTCCTGAGGCCACCACCCCAACCCAAGTCTCCAGGGCCAG
  CTATGGGCCCCATGGCTGCAGAGGGGGGTCCTCCACCAACACCCCTCAATGTCGTGCCTCCTGGGGCGCC
  AGTTGAGGAAGCAGAGGTGCGGCCACGGCCCATTATCCCAATGCTGTACGTGTTGCCCCGCACCAGCAGC
  ACCGATGGGGACAGGGAGCACTCTGCCCACGCACAGTTGGCACCCATGGAGCTGGGGCCAGAAGAGGAAA
  ACCAGGCCCAGGCTGGTGACTCACAGGGAACAACCCCTTTCTCTAAGCTGAAGGTGGAGATCAAAAAGAG
  CCGGCGTCACCCCTTGGGTCGGCCACCCACTCGATCCCCGCTGTCTGTGGTCAAGCAGGAGGCCTCAAGT
  GATGAAGAAGCCTTCCTGTTCTCAGGAGAGGATGATGTCACTGATCCTGAGGCCTTGAGATCGCTGCTGT
  CCCTGCAGTGGAAGAACAAGGCGGCCAGCTTCCAAGCCGAGAGGAAGTTCAATGCGGCAGCCGCCCTCAG
  TGAGCCCTACTGTGCCATCTGTACCCTCTTCTACCCCTACAGCCAGTCAGTACAGACAGAGCGGGACAGT
  GCTGTCCAGCCGCCCTCCAAAAGTGGCCAGAGGACACGGCCACTGATCCCTGAGATGTGCTTCACTTCAA
  GTGGGGAGAACACAGAGCCGCTGCCTGCTAACTCCTACGTCGGTGAGGACGGCACCAGTCCACTCATCTC
  CTGTGCCCACTGCTGCCTGCAGGTCCATGCCAGTTGCTATGGTGTCCGGCCTGAGTTGGCCAAAGAGGGC
  TGGACATGTTCCCGGTGTGCGGCCCACGCTTGGACTGCGGAATGCTGTTTGTGCAACCTGCGGGGGGGAG
  CGCTACAGAGGACCACAGAGCACAGGTGGATTCACGTGATCTGCGCCATTGCAGTCCCTGAGGTACGATT
  CCTCAATGTGATTGAACGCAACCCAGTGGACGTCAGTGCCATCCCTGAGCAGCGATGGAAACTGAAATGC
  ATTTACTGCCGCAAGCGTATGAAGAGAGTGTCGGGTGCCTGCATCCAGTGCTCCTATGAGCACTGCTCCA
  CATCCTTCCATGTGACCTGTGCGCATGCTGCTGGTGTCCTCATGGAACCCGATGACTGGCCCTATGTGGT
  GTCCATCACCTGCCTCAAGCACCGGGCCAGCGGAGCAGGGGGCCAGCTCCTGCGCACAGTGTCCCTGGGC
  CAGATCGTCATCACCAAGAACCGCAATGGGCTCTACTATCGCTGCCGGGTCATTGGCACCACTGCGCAGA
  CATTCTACGAGGTCAACTTTGACGATGGCTCCTACAGTGACAACCTGTACCCAGAGAGCATCACGAGCAG
  AGACTGCCTACGGCTAGGGCCACCTCCTGAGGGCGAGCTGGTGGAGCTTCGGTGGACAGACGGTAATCTG
  TACAGAGCCAGGTTTATCTCCATGGCCACCAGCCTCATCTACCAGGTAGAGTTTGAGGATGGGTCTCAGC
  TGACTGTGAAGCGTGGGGACATCTTCACACTGGAAGAGGAGCTGCCCAAGAGGGTGCGTTCCCGGCTGTC
  ACTGAGCACTGGCACACCACAGGAGCCCAGCTTCTCTGGAGACGATGTGAAGGCTGCCAAGCGCCCACGG
  GTGGCCTCAGTGCTGGCCACCACCACTGAGGACACAGGGCGTAGCCCAGAATACCTGTCCTTCATGGAGA
  GCCTGCTGCAGGCACAGGGCCGGCCTGGAGCACCCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211609 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGSEDHSAQNPSCKIMTFRPTMDEFRDFNRYVAYIESQGAHRAGLAKIIPPKEWKPRQTYDDIDDVVIPA
  PIQQVVTGQSGLFTQYNIQKKAMTVGEYRRLANSEKYCTPRHQDFDDLERKYWKNLTFVSPIYGADISGS
  LYDDDVAQWNIGNLRTILDMVERECGTIIEGVNTPYLYFGMWKTTFAWHTEDMDLYSINYLHFGEPKSWY
  AIPPEHGKRLERLAIGFFPGSSQGCDAFLRHKMTLISPIILKKYGIPFSRITQEAGEFMITFPYGYHAGF
  NHGFNCAESTNFATLRWIDYGKVATQCTCRKDMVKISMDVFVRILQPERYEQWKQGRDLTVLDHTRPTAL
  SSPELSSWSASRTSIKAKLLRRQISVKESRPWRKAEEERRREPTRRPGPASHRRRSQPKKSKPEESRSPG
  EATAGVSTLDEARGCSRGEAMPEDEEEEELLPSQGHEAEGVEEDGRGKPRPTKARNKKKTPSPSSPPLLS
  APPALFPTEEVLRPPPQPKSPGPAMGPMAAEGGPPPTPLNVVPPGAPVEEAEVRPRPIIPMLYVLPRTSS
  TDGDREHSAHAQLAPMELGPEEENQAQAGDSQGTTPFSKLKVEIKKSRRHPLGRPPTRSPLSVVKQEASS
  DEEAFLFSGEDDVTDPEALRSLLSLQWKNKAASFQAERKFNAAAALSEPYCAICTLFYPYSQSVQTERDS
  AVQPPSKSGQRTRPLIPEMCFTSSGENTEPLPANSYVGEDGTSPLISCAHCCLQVHASCYGVRPELAKEG
  WTCSRCAAHAWTAECCLCNLRGGALQRTTEHRWIHVICAIAVPEVRFLNVIERNPVDVSAIPEQRWKLKC
  IYCRKRMKRVSGACIQCSYEHCSTSFHVTCAHAAGVLMEPDDWPYVVSITCLKHRASGAGGQLLRTVSLG
  QIVITKNRNGLYYRCRVIGTTAQTFYEVNFDDGSYSDNLYPESITSRDCLRLGPPPEGELVELRWTDGNL
  YRARFISMATSLIYQVEFEDGSQLTVKRGDIFTLEEELPKRVRSRLSLSTGTPQEPSFSGDDVKAAKRPR
  VASVLATTTEDTGRSPEYLSFMESLLQAQGRPGAPF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172132
• ORF Size: 3261 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172132.3
• RefSeq Size: 4610 bp
• RefSeq ORF: 3261 bp
• Locus ID: 193796
• UniProt ID: Q91VY5
• Cytogenetics: 17 D
• MW: 121.6 kDa
• Gene Summary: Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate (By similarity).[UniProtKB/Swiss-Prot Function]