Plekhm1 (NM_183034) Mouse Tagged ORF Clone

CAT#: MR211592

Plekhm1 (Myc-DDK-tagged) - Mouse pleckstrin homology domain containing, family M (with RUN domain) member 1 (Plekhm1)

Product Images

Circular map for MR211592

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Plekhm1
• Synonyms: AP162; B2; BC038943; D330036J23Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211592 representing NM_183034
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCTCAGTGGAGAATGGCCTGGACCCTCGTGCTGCCATCCAGGTCATCAAGAAGAAGCTCGTGGGAT
  CCGTGAAGGCTCTGCAGAAACAGCATGTGTCTCTGGACACAGTGGTCACCAGTGAAGATGGAGATGCCAA
  CACCATGTGCAGTGCCCTGGAGGCTGTGTTCATCCACGGCCTGCATGCCAAGCACATCCGCGCCGAGGCT
  GGCGGCAAAAGGAAGAAGCACACTCACCAGAAGGCTCTGCCTCAGCCTGTCTTCTGGCCCCTTCTGAAAG
  CCATCACCCACAGACACATTGTCTCAGATTTGGAGCACCTGGTCTTCATCAACACAGATGTAGGCCGTTG
  CCGAGCCTGGCTGCGGTTGGCCCTCAATGACGGTCTGATGGAGTGCTATCTGAAACTGCTCCTGCAGGAA
  CCTGCACGGTTGTGCGAGTACTATCAGCCCACAGCCCTGCTTCGAGATGCCGAGGAGGCTGAGTTCCTCC
  TGAGCTTCCTGCAAGGACTAACATCCTTGTCCTTCGAACTCTCCTACAAGTCAGCCATCTTAAATGAATG
  GACACTCACCCCATTGTCGCTCTCTGGGCTTTGCCCCCTCTCTGAACTCGACCCTCTCACTACCTCTGGT
  GCAGAACTACAGCGGAAAGAGTCTCTGGATTCAATTTCCCATTCCTCGGGCTCTGAGGACATCGAAGTCC
  AACACTCAGGCCATAAGATCCGCAGGAACAGGAAGCTCACTGCCTCTTCCCTCAGCCTGGACACAGCCAG
  TTCATCCCAGCTGTCCTGCAGCCTAAATTCTGACAGCTGCCTGCTCCAAGAGAATGGTCCCAAGAGTCCA
  GACCATTCTGAGGAACCCATGTCCTATGACTCAGATCTGGGCATGGCAAACACCGATGACCCCGACAGGT
  CTCTGCAAGAGGTGTTGTCGGAATTCAGCAAAGCCCAGGTAAATTCTGCACCAAGCAGCGGACCAAACCA
  AGAGCCAGACACCCCCATGTTCCAGACGCCCCTCTCCCTCCATAGCCTAGCTACCAGCACACACCTGCAC
  TTTGAGGGATCTGAAGAGCTCTTTCCTGCCCACAAATCCTCTGGGACATCAAGTGGTGGCCATAAGCATC
  AGCTACTGCCCCAGGAGACCCCAGACGAGAAGCAGCTAGGCACTGCCCAAGCTGGCCCTGCACAGAGCAC
  TTCAGACCAGCAGCCTTCTAGTCCTGTGGGTGGAGCAGCTGGCCAAGGGAGTGGCCCTTGGAAGGCCCTG
  GAGTATGGCAGAGTCGGACCGAAGCTTGTGGTTTCCTCTCCTACGAGTCCGAAAGGCAAGAGCTGGATTT
  CTGAAGATGACTTCTGCCGACCTCCCCAGGAGCCTGCTCTAAAGAGCGCTGCAGGTCTCTGCACATCTCC
  TGTGCAAGACACTCCAGAGTCAAGGGCTGCTCTCCACGGGCCTTTTTCTCAAGGACCAAGAAAGAGCTGT
  TCCTTGGGGGCATTAGACAAAGCATGTGTGCCCTCACAGGCCTGTGGAAATGCCCAGCCAGCTCCAGCCC
  CAGCTCCAGCTCCAGCCCCAGCCCCAGCCCCAGCTCCAGGGGTGACACAAGACCATAAAAACTTCTGTGT
  GGTGCACCGAAGGCAAATGGGGCTGTCCAACCCATTCCGGGGCCTCATGAAGCTGGGCACAGTGGCCCGG
  CGAGGAGCCATGGGCATCTGGAAGGAATTCTTTTGTGAGCTGTCCCCGTTGGAGTTCCGCCTCTACTTGA
  GTGATGAGGAACGCACCTGTGTGGAGAGCTGCTCCCTGCTCCGATGTGAGGCTGTGGGGCCAGCACACAG
  TGATGGACGTTTTGAGTTGGTCTTCTCGGGCAAGAAGCTGGCTCTCCGTGCCTCCTCCCAAGACGAGGCT
  GAGGACTGGCTTGACCGCGTACGGGAGGCTCTGCAGAAGGTGCGCCCTCAGCAGGAAGATGAGTGGGTGA
  ATATCCAGTACCCAGACCAGGCTGAGGATGCCCCAGAGGCTCCACCAGACAGCCTCCCCCCCTATTCAAC
  TCTACTCCCCGAGCCTGCGGGTGCCCAGGGCATGCAGTTGGACTGGACATCTGCCCAGGTTCCAGAACCA
  GATGCTATCAAAGAGTCCCTTTTGTATCTGTATGCAGACCGGACCTGGGTCCCCTATATCTTTTCCCTGT
  CCTTGGAGTCTCTGAAATGTTTCCGAGTGAGAAACAATGAGAAGATGCTAAGTGATAGCCATGGAGTGGA
  GACCATCCGAGACATCCTGCCAGACACCAGCCTTGGGGGCCCAGCCTTCTTCAAAATCATCACGGCCAAG
  GCCGTCCTGAAACTGCAGGCCAAAAACACCGAGGAGGCCACCCACTGGAGAGACCTGGTCCGGAAAGTGC
  TGGCATCTTACCTAGAGTCAGCTGAGGAGGCTGTGACACTTGGTGGGAGTCTGGATGAAAAATGTCAGGA
  GGTGCTGAAGTTTGCTACCAGGGAGAACGGCTTCCTGCTACAGTACCTTGTGGCCATCCCGACCGAGAAG
  GGCCTCGACTCCCAGGGCTGCTTCTGCGCAGGCTGCTCCCGACAGATCGGCTTCTCCTTTGTACGACCCA
  AGCTCTGTGCCTTCTCTGGCCTGTATTACTGTGACTTCTGCCACCAAGACGATGCCTCAGTGATTCCAGC
  CAGAATCATCCACAACTGGGACCTCACGAAGCGCCCGGTATGCCGGCAGGCCCTGAAGTTCCTGGCCCAG
  ATCCGGGCCCAACCTCTCATCAACCTGCAGCTGGTGAACGCCTCTCTGTATGAGCATGTGGAACGCATGC
  ACCTCATTGGCAGGAGCCGGGAGCAGCTGAAACTTCTGGGGGACTACCTGGGCCTGTGCCGAAGTGGTGC
  TCTGAAGGAGTTGTGCAAAAGGCTGAGCCACAGGAATTATCTCCTGGAGTCTCCACACAGATTCAGTGTT
  GCTGACCTCCAGCAGATTGCAGAGGGGGTATATGAAGGGTTCCTCAAAGCCCTGATTGAATTCGCATCCC
  AGCATGTCTACCACTGTGACCTGTGCACCCAGCGAGGCTTCATCTGCCAGATCTGCCACCACCAGGACAT
  CATCTTTCCCTTTGAGTTTGACACCACAGTCAGGTGTGCTGAATGCAGAACTGTCTTCCATCAGAGCTGC
  CAGGCTGTGGTGAGGAAGGGCTGTCCCCGATGTGCCCGCCGGCGCAAGTACCAGGAACAGAACGTTGTCA
  GT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211592 representing NM_183034
  Red=Cloning site Green=Tags(s)
  
  MLSVENGLDPRAAIQVIKKKLVGSVKALQKQHVSLDTVVTSEDGDANTMCSALEAVFIHGLHAKHIRAEA
  GGKRKKHTHQKALPQPVFWPLLKAITHRHIVSDLEHLVFINTDVGRCRAWLRLALNDGLMECYLKLLLQE
  PARLCEYYQPTALLRDAEEAEFLLSFLQGLTSLSFELSYKSAILNEWTLTPLSLSGLCPLSELDPLTTSG
  AELQRKESLDSISHSSGSEDIEVQHSGHKIRRNRKLTASSLSLDTASSSQLSCSLNSDSCLLQENGPKSP
  DHSEEPMSYDSDLGMANTDDPDRSLQEVLSEFSKAQVNSAPSSGPNQEPDTPMFQTPLSLHSLATSTHLH
  FEGSEELFPAHKSSGTSSGGHKHQLLPQETPDEKQLGTAQAGPAQSTSDQQPSSPVGGAAGQGSGPWKAL
  EYGRVGPKLVVSSPTSPKGKSWISEDDFCRPPQEPALKSAAGLCTSPVQDTPESRAALHGPFSQGPRKSC
  SLGALDKACVPSQACGNAQPAPAPAPAPAPAPAPAPGVTQDHKNFCVVHRRQMGLSNPFRGLMKLGTVAR
  RGAMGIWKEFFCELSPLEFRLYLSDEERTCVESCSLLRCEAVGPAHSDGRFELVFSGKKLALRASSQDEA
  EDWLDRVREALQKVRPQQEDEWVNIQYPDQAEDAPEAPPDSLPPYSTLLPEPAGAQGMQLDWTSAQVPEP
  DAIKESLLYLYADRTWVPYIFSLSLESLKCFRVRNNEKMLSDSHGVETIRDILPDTSLGGPAFFKIITAK
  AVLKLQAKNTEEATHWRDLVRKVLASYLESAEEAVTLGGSLDEKCQEVLKFATRENGFLLQYLVAIPTEK
  GLDSQGCFCAGCSRQIGFSFVRPKLCAFSGLYYCDFCHQDDASVIPARIIHNWDLTKRPVCRQALKFLAQ
  IRAQPLINLQLVNASLYEHVERMHLIGRSREQLKLLGDYLGLCRSGALKELCKRLSHRNYLLESPHRFSV
  ADLQQIAEGVYEGFLKALIEFASQHVYHCDLCTQRGFICQICHHQDIIFPFEFDTTVRCAECRTVFHQSC
  QAVVRKGCPRCARRRKYQEQNVVS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_183034
• ORF Size: 3222 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_183034.2
• RefSeq Size: 5144 bp
• RefSeq ORF: 3225 bp
• Locus ID: 353047
• UniProt ID: Q7TSI1
• Cytogenetics: 11 E1
• MW: 118.5 kDa
• Gene Summary: Proposed to act as a multivalent adapter protein that regulates Rab7-dependent and HOPS complex-dependent fusion events in the endolysosomal system and couples autophagic and the endocytic trafficking pathways. Required for late stages of endolysosomal maturation, facilitating both endocytosis-mediated degradation of growth factor receptors and autophagosome clearance. Seems to be involved in the terminal maturation of autophagosomes and to mediate autophagosome-lysosome fusion (PubMed:25498145). Positively regulates lysosome peripheral distribution and ruffled border formation in osteoclasts (PubMed:27777970). May be involved in negative regulation of endocytic transport from early endosome to late endosome/lysosome implicating its association with Rab7 (By similarity). May have a role in sialyl-lex-mediated transduction of apoptotic signals (By similarity). Involved in bone resorption (PubMed:27777970).[UniProtKB/Swiss-Prot Function]