Cnksr2 (NM_177751) Mouse Tagged ORF Clone

CAT#: MR211490

Cnksr2 (Myc-DDK-tagged) - Mouse connector enhancer of kinase suppressor of Ras 2 (Cnksr2)

Product Images

Circular map for MR211490

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cnksr2
• Synonyms: Cnk2; Ksr2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211490 representing NM_177751
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTGATAATGGAACCGGTGAGCAAATGGTCTCCGAGTCAAGTAGTGGACTGGATGAAAGGTCTTG
  ATGACTGTTTGCAGCAGTACATTAAGAACTTTGAGAGAGAGAAGATCAGTGGAGACCAGCTGCTACGCAT
  TACACATCAGGAGCTAGAAGATCTGGGAGTCAGCCGTATTGGCCATCAGGAGCTGATCTTGGAAGCAGTT
  GACCTTTTGTGTGCACTGAATTATGGCTTAGAAACAGAAAATCTAAAAACACTTTCTCACAAGTTGAATG
  CATCTGCCAAAAATCTACAAAACTTCATAACAGGAAGGAGAAGGAGCGGCCATTATGATGGGAGGACCAG
  CCGAAAATTACCAAATGACTTTTTGACCTCAGTTGTGGATCTGATTGGAGCAGCCAAGAGTCTTCTTGCT
  TGGTTGGACAGGTCACCATTTGCTGCTGTGACAGACTATTCAGTCACAAGAAATAACGTCATACAGCTCT
  GCTTGGAATTAACAACAATTGTGCAACAGGATTGCACTGTATATGAAACTGAAAATAAAATTCTTCATGT
  GTGTAAAACTCTCTCTGGAGTCTGTGACCACATCATATCCCTGTCATCAGATCCTCTGGTTTCACAGTCT
  GCTCACCTGGAAGTGATTCAGCTGGCAAATATCAAACCAAGTGAAGGGCTGGGTATGTATATTAAATCCA
  CATATGATGGCCTCCATGTAATTACCGGGACCACAGAAAATTCACCTGCAGATCGCTGTAAGAAAATCCA
  TGCTGGTGATGAAGTAATCCAAGTGAACCATCAGACTGTGGTGGGGTGGCAGTTGAAAAATTTGGTGAAT
  GCACTACGAGAGGACCCGAGTGGTGTTATCTTAACTTTGAAAAAGCGACCTCAGAGCATGCTTACCTCAG
  CACCAGCTTTACTGAAAAATATGAGATGGAAGCCCCTTGCTCTGCAGCCTCTTATACCTAGAAGTCCCAC
  AAGCAGCGTTGCCACGCCCTCCAGCACCATCAGTACACCCACCAAAAGAGACAGTTCTGCTCTCCAGGAT
  CTCTACATCCCCCCTCCTCCTGCAGAACCATATATTCCCAGGGATGAAAAAGGAAACCTTCCTTGTGAAG
  ACCTCAGAGGACATATGGTGGGCAAGCCAGTTCATAAGGGGTCTGAATCCCCAAATTCATTTCTGGACCA
  AGAATATCGAAAGAGGTTTAACATTGTTGAAGAAGATACTGTCTTATATTGCTATGAATATGAAAAGGGA
  CGATCAAGTAGTCAAGGAAGACGGGAAAGCACACCAACCTATGGCAAGCTACGACCTATATCTATGCCAG
  TGGAATATAATTGGGTGGGGGACTATGAAGATCCAAATAAGATGAAGAGAGATAGTAGAAGAGAAAACTC
  TCTACTTCGATACATGAGCAATGAAAAAATTGCTCAAGAAGAATACATGTTTCAGAGAAACAGCAAAAAA
  GACACAGGGAAGAAGTCAAAAAAGAAGGGTGATAAGAGTAATAGCCCAGCCCACTACTCATTGCTGCCTA
  GTTTGCAAATGGATGCATTGAGACAAGACATCATGGGCACACCAGTGCCAGAAACCACACTGTACCATAC
  ATTTCAACAGTCCTCTCTGCAGCACAAATCAAAGAAGAAAAACAAAGGAGCTATATCAGGCAAGAGCAAA
  AGAAGAATTTCTTGCAAGGATCTTGGCCGTGGTGACTGTGAAGGATGGCTTTGGAAAAAGAAAGATGCAA
  AGAGCTATTTTTCACAGAAATGGAAGAAGTACTGGTTTGTCCTAAAGGATGCATCCCTATACTGGTATAT
  TAATGAAGAGGATGAAAAAGCAGAAGGATTCATCAGTCTGCCTGAATTTAAAATCGATAGAGCCAGTGAA
  TGCCGCAAGAAATATGCATTCAAAGCCTGCCATCCTAAAATCAAAAGCTTTTATTTTGCTGCTGAACATC
  TTGATGACATGAACAGGTGGCTTAACAGAATTAACATGCTGACTGCAGGATATGCAGAAAGAGAGAGGAT
  TAAACAAGAACAAGATTACTGGAGCGAGAGTGACAAAGAAGAAGCAGATACTCCATCGACACCAAAACAA
  GACAGCCCTCCGCCCCCCTATGACACATACCCACGGCCTCCCTCTATGAGTTGTGCCAGTCCTTATGTGG
  AAGCAAAACACAGCCGGCTTTCCTCCACAGAGACCTCTCAGTCTCAGTCTTCACACGAGGAGTTCCGCCA
  GGAAGTCACTGGGAGCAGTGCTGTGTCTCCCATTCGCAAGACAGCCAGTCAGCGCCGCTCCTGGCAGGAT
  TTAATCGAGACACCGCTGACAAGCTCAGGCTTACACTATCTTCAGACTCTGCCTCTGGAAGATTCTGTCT
  TCTCGGATTCAGCAGCCATCTCCCCTGAGCACAGGCGGCAGTCCACTCTTCCAACTCAGAAGTGCCACCT
  CCAGGATCATTATGGCCCATACCCTTTAGCAGAGAGCGAGAGGATGCAAGTGTTAAACGGCAATGGGGGC
  AAGCCTCGAAGTTTTACTCTGCCTCGAGATAGCGGGTTCAACCATTGCTGTCTGAACACACCAGTTAGTG
  CCTGTGACCCACAAGATGACATACAGCCACCAGAGGTGGAGGAAGAAGAGGAGGAGGAAGAAGAGGAAGC
  AGCAGGAGAAAACGTTGGAGAGAAAAATGAAAACAGGGAAGAAAAATTAGGAGACTCCTTGCAAGATTTA
  TACAGGGCACTGGAGGAAGCCAGTCTGTCACCACTAGGAGAACATCGCATTTCAACCAAGATGGAATACA
  AACTATCATTCATAAAACGGTGTAATGATCCTGTGATGAATGAGAAACTGCACAGGCTGAGAATTCTTAA
  AAGCACTTTAAAGGCCAGAGAAGGGGAAGTAGCCATTATTGATAAAGTCCTAGACAATCCAGACTTGACT
  TCTAAAGAATTCCAACAATGGAAGCAGATGTACCTCGACCTTTTCTTGGATATCTGTCAAAGCACCACCT
  CAAATGACCCACTAAGTATCTCTTCTGAAGTAGATGTTCTCACTTCCTCTCTAACACACACTCACTCATA
  CATTGAGACACATGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211490 representing NM_177751
  Red=Cloning site Green=Tags(s)
  
  MALIMEPVSKWSPSQVVDWMKGLDDCLQQYIKNFEREKISGDQLLRITHQELEDLGVSRIGHQELILEAV
  DLLCALNYGLETENLKTLSHKLNASAKNLQNFITGRRRSGHYDGRTSRKLPNDFLTSVVDLIGAAKSLLA
  WLDRSPFAAVTDYSVTRNNVIQLCLELTTIVQQDCTVYETENKILHVCKTLSGVCDHIISLSSDPLVSQS
  AHLEVIQLANIKPSEGLGMYIKSTYDGLHVITGTTENSPADRCKKIHAGDEVIQVNHQTVVGWQLKNLVN
  ALREDPSGVILTLKKRPQSMLTSAPALLKNMRWKPLALQPLIPRSPTSSVATPSSTISTPTKRDSSALQD
  LYIPPPPAEPYIPRDEKGNLPCEDLRGHMVGKPVHKGSESPNSFLDQEYRKRFNIVEEDTVLYCYEYEKG
  RSSSQGRRESTPTYGKLRPISMPVEYNWVGDYEDPNKMKRDSRRENSLLRYMSNEKIAQEEYMFQRNSKK
  DTGKKSKKKGDKSNSPAHYSLLPSLQMDALRQDIMGTPVPETTLYHTFQQSSLQHKSKKKNKGAISGKSK
  RRISCKDLGRGDCEGWLWKKKDAKSYFSQKWKKYWFVLKDASLYWYINEEDEKAEGFISLPEFKIDRASE
  CRKKYAFKACHPKIKSFYFAAEHLDDMNRWLNRINMLTAGYAERERIKQEQDYWSESDKEEADTPSTPKQ
  DSPPPPYDTYPRPPSMSCASPYVEAKHSRLSSTETSQSQSSHEEFRQEVTGSSAVSPIRKTASQRRSWQD
  LIETPLTSSGLHYLQTLPLEDSVFSDSAAISPEHRRQSTLPTQKCHLQDHYGPYPLAESERMQVLNGNGG
  KPRSFTLPRDSGFNHCCLNTPVSACDPQDDIQPPEVEEEEEEEEEEAAGENVGEKNENREEKLGDSLQDL
  YRALEEASLSPLGEHRISTKMEYKLSFIKRCNDPVMNEKLHRLRILKSTLKAREGEVAIIDKVLDNPDLT
  SKEFQQWKQMYLDLFLDICQSTTSNDPLSISSEVDVLTSSLTHTHSYIETHV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177751
• ORF Size: 3096 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177751.3, NP_808419.1
• RefSeq Size: 5434 bp
• RefSeq ORF: 3099 bp
• Locus ID: 245684
• UniProt ID: Q80YA9
• Cytogenetics: X F4
• MW: 117.8 kDa
• Gene Summary: May function as an adapter protein or regulator of Ras signaling pathways.[UniProtKB/Swiss-Prot Function]