Tnpo3 (NM_177296) Mouse Tagged ORF Clone

CAT#: MR211196

Tnpo3 (Myc-DDK-tagged) - Mouse transportin 3 (Tnpo3)

Product Images

Circular map for MR211196

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tnpo3
• Synonyms: 5730544L10Rik; C81142; C430013M08Rik; D6Ertd313e; mKIAA4133; Trn-SR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211196 representing NM_177296
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGAGCCAAGCCAACCTTGCAGCTCGTGTACCAGGCGGTGCAAGCGCTTTACCACGACCCGGATC
  CCAGCGGAAAGGAGCGCGCCTCGTTTTGGCTTGGGGAGCTGCAGCGTTCGGTTCACGCTTGGGAGATTTC
  TGATCAATTGTTACAGATCCGACAGGATGTGGAATCATGCTATTTCGCTGCCCAGACCATGAAAATGAAG
  ATTCAGACCTCATTTTATGAGCTCCCCACAGACTCTCATGCTTCTTTAAGGGACTCATTGCTAACTCACA
  TTCAGAACTTGAAAGACTTGTCGCCTGTCATTGTAACACAGCTGGCTTTAGCAATAGCAGACCTCGCCCT
  ACAGATGCCTTCTTGGAAAGGATGCGTACAAACATTGGTAGAAAAATATAGCAATGACGTAACTTCTTTA
  CCTTTTTTGCTGGAAATCCTTACAGTGTTACCCGAAGAAGTACATAGTCGTTCCTTGCGGATTGGGGCCA
  ACAGGCGGACAGAAATTATAGAAGACTTAGCCTTCTACTCTAGTACCGTGGTATCTTTATTGATGACATG
  TGTAGAGAAAGCAGGAACCGATGAGAAGATGCTTATGAAGGTCTTCCGATGTTTGGGAAGTTGGTTTAAC
  TTGGGGGTTTTGGACAGTAACTTCATGGCTAATAATAAGTTATTAGCACTCCTTTTTGAGGTCTTGCAAC
  AAGATAAGACCTCATCTAACCTACACGAAGCTGCTTCAGACTGTGTATGCTCAGCTCTCTATGCTATTGA
  AAATGTAGAGACTAACTTGCCATTGGCCATGCAGCTTTTTCAGGGAGTCCTGACATTGGAGACTGCATAT
  CATATGGCTGTGGCACGGGAAGATTTGGACAAAGTTCTGAATTACTGCCGAATTTTCACCGAACTATGTG
  AAACTTTCCTGGAAAAAATTGTCTGTACTCCAGGCCAAGGTCTGGGAGATCTTCGAACTTTGGAATTGCT
  TCTTATCTGTGCAGGCCACCCTCAGTATGAGGTAGTAGAAATTTCCTTTAACTTTTGGTACCGACTAGGA
  GAACATTTATACAAAACCAATGATGAAGTTATTCACAGCATCTTCAAAGCGTACATTCAGAGGCTGCTTC
  ATGCCTTGGCTCGACACTGCCAGTTGGAACCAGACCATGAGGGAGTTCCTGAAGAGACCGATGACTTTGG
  GGAGTTTCGAATGAGAGTGTCAGACCTGGTGAAGGACTTGATCTTCCTGATTGGATCTATGGAGTGTTTT
  GCTCAGTTGTATTCTACTCTGAAGGAAGGCAACCCACCCTGGGAGGTGACAGAAGCGGTTCTCTTTATCA
  TGGCTGCTATAGCAAAGAGTGTGGATCCGGAGAACAACCCTACACTTGTGGAGGTACTAGAGGGAGTTGT
  CCATCTCCCAGAGACCGTGCATACAGCTGTGCGGTACACTAGCATTGAGTTGGTTGGTGAGATGAGTGAA
  GTGGTGGACCGGAATCCCCAGTTCCTGGACCCTGTATTGGGCTATTTGATGAAAGGCCTGTGTGAAAAGC
  CTCTGGCTTCTGCTGCAGCCAAAGCCATTCATAACATTTGTTCCGTTTGTCGCGATCATATGGCTCAGCA
  CTTTAATGGACTCCTAGAAATTGCCCACTCCCTTGATTCTTTCATGTTGTCTCCCGAAGCTGCTGTGGGT
  TTGCTAAAAGGAACAGCTCTTGTCCTAGCAAGACTACCTTTGGATAAGATTACAGAATGTCTAAGTGAGT
  TATGTTCTGTTCAGGTTATGGCATTGAAAAAGCTGTTGTCTCAGGAGCCCAGCAATGGCATTTCTTCAGA
  TCCCACTGTCTTCTTAGATCGCCTGGCAGTAATATTTAGACACACCAATCCTATTGTAGAAAATGGACAG
  ACTCATCCATGCCAAAAAGTCATCCAGGAAATATGGCCAGTTTTGTCTGAGACTCTAAATAAGCACCGAG
  CCGACAATCGAATTGTAGAGCGTTGTTGTAGATGCCTGCGCTTTGCGGTTCGTTGTGTAGGCAAAGGATC
  TGCAGCACTGCTACAGCCACTAGTTACACAGATGGTGAATGTGTACCATGTACATCAGCATTCCTGTTTC
  CTCTACCTTGGCAGTATCCTTGTGGATGAGTACGGCATGGAAGAAGGCTGTCGGCAGGGGTTATTAGACA
  TGCTCCAGGCGCTATGCATTCCCACCTTTCAGCTCCTAGAACAGCAGAATGGCCTCCAAAATCACCCTGA
  CACTGTAGATGACCTATTCAGGTTAGCCACCCGATTTATTCAGCGAAGCCCTGTCACCTTGCTGAGGAGC
  CAGGTTGTCATCCCAATCTTACAGTGGGCCATTGCATCAACCACTTTGGACCACCGAGATGCCAATTCCA
  GTGTCATGAGATTTCTGCGAGACCTCATCCACACAGGAGTAGCCAATGATCATGAAGAAGATTTTGAATT
  GCGGAAGGAACTAATTGGACAGGTGATGAGCCAGCTTGGCCAGCAACTTGTCAGCCAGCTGCTCCACACA
  TGCTGCTTTTGTCTTCCCCCCTACACCCTACCCGACGTGGCTGAAGTGCTCTGGGAGATCATGCAGGTTG
  ACAGACCGACTTTCTGTCGGTGGCTAGAGAATTCCTTGAAAGGTTTGCCAAAAGAGACCACAGTGGGAGC
  TGTCACAGTGACACATAAACAACTTACAGATTTCCACAAGCAAGTCACTAGTGCCGAGGAATGTAAGCAA
  GTTTGCTGGGCCTTGAGAGACTTCACCAGGTTGTTTCGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211196 representing NM_177296
  Red=Cloning site Green=Tags(s)
  
  MEGAKPTLQLVYQAVQALYHDPDPSGKERASFWLGELQRSVHAWEISDQLLQIRQDVESCYFAAQTMKMK
  IQTSFYELPTDSHASLRDSLLTHIQNLKDLSPVIVTQLALAIADLALQMPSWKGCVQTLVEKYSNDVTSL
  PFLLEILTVLPEEVHSRSLRIGANRRTEIIEDLAFYSSTVVSLLMTCVEKAGTDEKMLMKVFRCLGSWFN
  LGVLDSNFMANNKLLALLFEVLQQDKTSSNLHEAASDCVCSALYAIENVETNLPLAMQLFQGVLTLETAY
  HMAVAREDLDKVLNYCRIFTELCETFLEKIVCTPGQGLGDLRTLELLLICAGHPQYEVVEISFNFWYRLG
  EHLYKTNDEVIHSIFKAYIQRLLHALARHCQLEPDHEGVPEETDDFGEFRMRVSDLVKDLIFLIGSMECF
  AQLYSTLKEGNPPWEVTEAVLFIMAAIAKSVDPENNPTLVEVLEGVVHLPETVHTAVRYTSIELVGEMSE
  VVDRNPQFLDPVLGYLMKGLCEKPLASAAAKAIHNICSVCRDHMAQHFNGLLEIAHSLDSFMLSPEAAVG
  LLKGTALVLARLPLDKITECLSELCSVQVMALKKLLSQEPSNGISSDPTVFLDRLAVIFRHTNPIVENGQ
  THPCQKVIQEIWPVLSETLNKHRADNRIVERCCRCLRFAVRCVGKGSAALLQPLVTQMVNVYHVHQHSCF
  LYLGSILVDEYGMEEGCRQGLLDMLQALCIPTFQLLEQQNGLQNHPDTVDDLFRLATRFIQRSPVTLLRS
  QVVIPILQWAIASTTLDHRDANSSVMRFLRDLIHTGVANDHEEDFELRKELIGQVMSQLGQQLVSQLLHT
  CCFCLPPYTLPDVAEVLWEIMQVDRPTFCRWLENSLKGLPKETTVGAVTVTHKQLTDFHKQVTSAEECKQ
  VCWALRDFTRLFR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177296
• ORF Size: 2769 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177296.5
• RefSeq Size: 3971 bp
• RefSeq ORF: 2772 bp
• Locus ID: 320938
• UniProt ID: Q6P2B1
• Cytogenetics: 6 12.36 cM
• MW: 104.6 kDa
• Gene Summary: Seems to function in nuclear protein import as nuclear transport receptor. In vitro, mediates the nuclear import of splicing factor SR proteins RBM4, SFRS1 and SFRS2, by recognizing phosphorylated RS domains (By similarity).[UniProtKB/Swiss-Prot Function]