Hk2 (NM_013820) Mouse Tagged ORF Clone

CAT#: MR211170

Hk2 (Myc-DDK-tagged) - Mouse hexokinase 2 (Hk2)

Product Images

Circular map for MR211170

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hk2
• Synonyms: AI642394; HKII
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211170 representing NM_013820
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCGCCTCGCATATGATCGCCTGCTTATTCACGGAGCTCAACCAAAACCAAGTGCAGAAGGTTGACC
  AGTATCTCTACCACATGCGTCTCTCAGATGAGACCCTTCTGGAGATTTCTAGGCGGTTCCGGAAGGAGAT
  GGAGAAGGGGCTAGGAGCTACCACACACCCTACAGCAGCTGTGAAGATGCTGCCCACCTTTGTGAGGTCA
  ACTCCGGATGGGACAGAACATGGCGAGTTCCTGGCTCTGGACCTTGGAGGAACCAACTTCCGTGTGCTCC
  GAGTAAGGGTGACAGACAATGGTCTCCAGAGGGTGGAGATGGAGAACCAGATCTACGCCATTCCCGAGGA
  CATCATGCGGGGCAGTGGAACCCAGCTGTTTGACCACATTGCCGAATGCCTGGCCAACTTCATGGACAAG
  CTACAGATCAAAGAGAAGAAGCTCCCGCTGGGTTTCACCTTCTCGTTCCCCTGCCACCAGACGAAACTGG
  ATGAGAGTTTCTTGGTCTCATGGACTAAGGGGTTCAAGTCCAGTGGCGTGGAAGGCAGAGATGTGGTGGA
  TCTGATCCGGAAGGCCATCCAGCGCAGAGGGGACTTTGACATTGACATTGTGGCTGTGGTGAATGACACG
  GTTGGGACCATGATGACTTGTGGTTATGATGATCAGAACTGCGAGATTGGTCTCATTGTGGGTACTGGCA
  GTAATGCCTGCTACATGGAGGAGATGCGTCACATTGACATGGTGGAGGGCGACGAGGGACGGATGTGCAT
  CAACATGGAGTGGGGGGCCTTTGGGGACGACGGTACACTCAATGACATCCGAACTGAGTTTGACAGAGAG
  ATCGACATGGGCTCACTGAACCCTGGGAAGCAGCTGTTTGAGAAGATGATCAGCGGGATGTACATGGGGG
  AGCTGGTCAGACTCATCCTGGTGAAGATGGCCAAGGCCGAGCTGCTGTTCCAAGGGAAACTCAGCCCAGA
  GCTCCTTACCACTGGCTCCTTCGAGACCAAAGATGTCTCGGATATTGAAGACGATAAGGACGGAATTCAG
  AAGGCCTACCAGATCCTGGTGCGCCTGGGTCTGAGCCCGCTGCAGGAGGACTGTGTGGCCACGCACCGAA
  TCTGCCAGATTGTGTCCACGCGCTCAGCCAGCCTGTGTGCAGCCACCCTGGCCGCGGTGCTGTGGCGGAT
  CAAAGAGAACAAGGGCGAGGAGCGACTCCGCTCCACCATCGGCGTGGATGGCTCTGTCTACAAGAAACAT
  CCCCATTTTGCCAAGCGTCTCCATAAGGCAGTGAGGAGGCTGGTGCCCGATTGTGATGTCCGCTTCCTCC
  GCTCTGAGGATGGCAGCGGCAAGGGGGCGGCTATGGTGACGGCGGTGGCTTACCGTCTGGCTGACCAACA
  CCGGGCCCGCCAGAAGACCCTGGAGTCTCTGAAGCTGAGCCATGAACAGCTGCTGGAGGTTAAGAGAAGG
  ATGAAGGTGGAAATGGAGCAGGGTCTGAGCAAGGAGACGCATGAGGCCGCCCCTGTGAAGATGTTGCCCA
  CTTATGTGTGTGCCACTCCAGACGGTACAGAGAAAGGAGACTTCCTGGCCTTGGATCTTGGAGGAACAAA
  CTTCCGGGTCCTGCTGGTGCGCGTGCGTAATGGCAAGCGAAGGGGCGTAGAGATGCATAACAAGATCTAC
  TCCATCCCACAGGAAGTCATGCATGGCACTGGAGAAGAGCTTTTCGACCACATTGTCCAGTGCATCGCGG
  ACTTCCTTGAGTACATGGGCATGAAGGGCGTGTCCCTACCTTTGGGTTTCACCTTCTCCTTCCCTTGCCA
  GCAGAACAGCCTGGACCAGAGCATCCTCCTCAAGTGGACAAAGGGATTCAAGGCATCCGGCTGCGAGGGT
  GAAGATGTGGTCACCTTGCTGAAGGAAGCCATTCGCCGGCGAGAGGAGTTTGACCTGGATGTGGTGGCCG
  TGGTAAATGACACAGTTGGGACTATGATGACTTGTGGCTATGAAGACCCTCACTGTGAAGTTGGCCTCAT
  TGTTGGCACTGGAAGCAATGCCTGCTACATGGAGGAGATGCGTAATGTGGAACTGGTGGACGGAGAGGAG
  GGGCGGATGTGTGTCAACATGGAGTGGGGAGCGTTCGGGGACAACGGCTGCCTGGATGACCTGCGCACAG
  TGTTTGACGTCGCTGTGGACGAGCTCTCTCTCAACCCTGGCAAACAGAGGTTTGAGAAGATGATCAGCGG
  CATGTACTTGGGCGAGATTGTGCGCAACATCCTGATCGATTTCACAAAGCGGGGGCTGCTCTTCCGTGGC
  CGCATCTCAGAGCGCCTCAAGACAAGGGGAATCTTCGAAACTAAGTTCCTGTCTCAGATCGAGAGTGACT
  GCCTGGCCCTGCTACAGGTCCGAGCCATCTTGCGCCACCTAGGGCTGGAGAGCACGTGTGACGACAGCAT
  CATTGTGAAGGAGGTGTGCACTGTGGTCGCCCGGCGCGCGGCCCAGCTCTGTGGCGCAGGCATGGCCGCC
  GTGGTGGACAAGATAAGAGAGAACCGTGGACTGGACAACCTCAAAGTGACGGTGGGCGTGGACGGGACAC
  TGTACAAGCTTCATCCTCACTTTGCCAAAGTCATGCATGAGACGGTGAGAGATCTGGCTCCGAAATGTGA
  CGTGTCCTTCCTGGAATCCGAGGACGGCAGTGGGAAGGGAGCGGCTCTCATCACTGCTGTGGCCTGTCGC
  ATCCGGGAGGCCGGGCAGAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211170 representing NM_013820
  Red=Cloning site Green=Tags(s)
  
  MIASHMIACLFTELNQNQVQKVDQYLYHMRLSDETLLEISRRFRKEMEKGLGATTHPTAAVKMLPTFVRS
  TPDGTEHGEFLALDLGGTNFRVLRVRVTDNGLQRVEMENQIYAIPEDIMRGSGTQLFDHIAECLANFMDK
  LQIKEKKLPLGFTFSFPCHQTKLDESFLVSWTKGFKSSGVEGRDVVDLIRKAIQRRGDFDIDIVAVVNDT
  VGTMMTCGYDDQNCEIGLIVGTGSNACYMEEMRHIDMVEGDEGRMCINMEWGAFGDDGTLNDIRTEFDRE
  IDMGSLNPGKQLFEKMISGMYMGELVRLILVKMAKAELLFQGKLSPELLTTGSFETKDVSDIEDDKDGIQ
  KAYQILVRLGLSPLQEDCVATHRICQIVSTRSASLCAATLAAVLWRIKENKGEERLRSTIGVDGSVYKKH
  PHFAKRLHKAVRRLVPDCDVRFLRSEDGSGKGAAMVTAVAYRLADQHRARQKTLESLKLSHEQLLEVKRR
  MKVEMEQGLSKETHEAAPVKMLPTYVCATPDGTEKGDFLALDLGGTNFRVLLVRVRNGKRRGVEMHNKIY
  SIPQEVMHGTGEELFDHIVQCIADFLEYMGMKGVSLPLGFTFSFPCQQNSLDQSILLKWTKGFKASGCEG
  EDVVTLLKEAIRRREEFDLDVVAVVNDTVGTMMTCGYEDPHCEVGLIVGTGSNACYMEEMRNVELVDGEE
  GRMCVNMEWGAFGDNGCLDDLRTVFDVAVDELSLNPGKQRFEKMISGMYLGEIVRNILIDFTKRGLLFRG
  RISERLKTRGIFETKFLSQIESDCLALLQVRAILRHLGLESTCDDSIIVKEVCTVVARRAAQLCGAGMAA
  VVDKIRENRGLDNLKVTVGVDGTLYKLHPHFAKVMHETVRDLAPKCDVSFLESEDGSGKGAALITAVACR
  IREAGQR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013820
• ORF Size: 2751 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013820.3, NP_038848.1
• RefSeq Size: 5524 bp
• RefSeq ORF: 2754 bp
• Locus ID: 15277
• UniProt ID: O08528
• Cytogenetics: 6 35.94 cM
• MW: 103 kDa
• Gene Summary: Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D-fructose 6-phosphate, respectively) (By similarity). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (By similarity). Plays a key role in maintaining the integrity of the outer mitochondrial membrane by preventing the release of apoptogenic molecules from the intermembrane space and subsequent apoptosis (PubMed:18350175).[UniProtKB/Swiss-Prot Function]