Sun1 (NM_024451) Mouse Tagged ORF Clone

CAT#: MR211159

Sun1 (Myc-DDK-tagged) - Mouse Sad1 and UNC84 domain containing 1 (Sun1)

Product Images

Circular map for MR211159

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sun1
• Synonyms: 4632417G13Rik; 5730434D03Rik; mKIAA0810; Unc84a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211159 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTTTTCTCGGCTGCACACGTACACCCCACCCCAGTGTGTGCCGGAGAACACTGGCTACACTTACG
  CACTCAGTTCTAGTTACTCGTCGGATGCTCTGGATTTTGAAACTGAGCACAAGTTGGAACCTGTATTTGA
  CTCTCCAAGGATGTCCCGCCGCAGCTTGCGTCTGGTCACAACAGCTTCGTACAGCAGTGGGGACAGCCAG
  GCTATTGATTCGCACATTAGCACCAGCAGGGCCACCCCCGCCAAGGGGAGAGAAACCAGGACAGTCAAAC
  AGAGAAGAAGTGCAAGCAAGCCAGCTTTTAGTATCAACCACCTGTCAGGGAAGGGCTTGTCCTCGAGCAC
  AAGCCATGACAGCTCTTGCAGCCTGCGGAGTGCCACGGTGCTGCGGCACCCTGTGCTAGATGAGTCCCTG
  ATTCGTGAGCAGACCAAAGTGGACCACTTCTGGGGTCTCGATGATGATGGTGACCTTAAAGGTGGAAATA
  AAGCTGCCACTCAGGGAAATGGTGAACTGGCAGCAGAGGTGGCGAGCAGCAATGGATACACTTGCCGTGA
  CTGCAGGATGCTCTCAGCGCGCACTGACGCACTCACAGCCCACTCTGCCATCCACGGGACCACCTCCAGG
  GTGTACTCCAGAGACAGGACTCTCAAACCACGCGGAGTGTCCTTTTACCTGGATAGGACTCTGTGGCTGG
  CCAAGTCCACCTCCTCATCCTTTGCATCATTTATAGTTCAACTTTTCCAAGTGGTTTTAATGAAGCTCAA
  TTTTGAAACTTACAAATTGAAAGGCTATGAATCCAGAGCTTATGAATCACAGAGCTATGAGACAAAGAGC
  CATGAGTCAGAAGCCCATCTCGGTCACTGTGGGAGGATGACTGCCGGAGAACTTTCCAGAGTGGACGGGG
  AGTCCCTGTGCGATGACTGTAAGGGGAAGAAGCACCTTGAGATACACACAGCCACCCACTCGCAACTGCC
  CCAGCCACACAGGGTGGCCGGGGCCATGGGGCGCCTCTGCATCTATACAGGTGACCTCTTGGTTCAAGCA
  CTGCGAAGGACTAGAGCTGCCGGGTGGTCTGTGGCCGAGGCCGTGTGGTCGGTGCTCTGGCTGGCTGTCT
  CTGCTCCAGGGAAGGCAGCCTCGGGAACCTTCTGGTGGCTAGGGAGCGGCTGGTACCAATTTGTTACTTT
  GATTTCTTGGCTGAATGTCTTTCTTCTTACCAGGTGCCTTCGAAATATTTGCAAGGTTTTTGTCTTGCTC
  CTCCCACTCCTACTTTTACTAGGTGCTGGTGTCTCCCTGTGGGGCCAGGGAAACTTCTTCTCACTCCTAC
  CAGTGCTGAACTGGACGGCCATGCAGCCAACACAGAGGGTGGACGATTCCAAGGGCATGCATAGACCTGG
  CCCTCTTCCCCCGAGCCCACCTCCAAAGGTTGATCACAAGGCTTCCCAGTGGCCTCAGGAGAGTGACATG
  GGGCAGAAGGTAGCTTCTTTGAGTGCGCAGTGCCACAACCATGATGAGAGACTTGCAGAGCTGACAGTCC
  TGCTTCAGAAACTACAGATACGGGTAGACCAAGTGGATGACGGCAGGGAAGGGCTGTCACTGTGGGTCAA
  GAATGTGGTTGGACAGCACCTGCAGGAGATGGGCACCATAGAACCACCTGATGCTAAGACTGACTTCATG
  ACTTTCCACCATGACCATGAAGTGCGTCTCTCCAACTTGGAAGATGTTCTTAGAAAACTGACAGAAAAAT
  CTGAGGCTATCCAGAAGGAGCTGGAAGAAACCAAGCTGAAAGCAGGCAGCAGGGATGAAGAGCAGCCCCT
  CCTTGACCGTGTGCAGCACCTAGAACTGGAACTGAACCTGTTGAAGTCACAGCTGTCAGACTGGCAGCAT
  CTGAAGACCAGCTGTGAGCAGGCTGGGGCCCGCATCCAGGAGACTGTGCAGCTCATGTTCTCTGAGGATC
  AGCAGGGCGGTTCCCTCGAGTGGCTATTAGAGAAGCTTTCTTCTCGGTTCGTGAGCAAGGATGAGCTGCA
  GGTGCTCTTACATGACCTTGAGCTGAAACTGCTGCAGAATATCACACACCACATCACCGTGACAGGACAG
  GCCCCGACATCCGAGGCTATTGTGTCTGCCGTGAATCAGGCAGGGATTTCAGGAATCACAGAAGCGCAAG
  CACATATCATTGTGAACAATGCTCTGAAGCTGTACTCCCAAGACAAGACGGGGATGGTGGACTTTGCTCT
  GGAGTCTGGAGGTGGCAGCATCCTAAGCACTCGGTGCTCTGAGACCTATGAGACCAAGACGGCACTGCTG
  AGCCTGTTTGGGGTCCCACTGTGGTACTTCTCACAGTCACCTCGAGTGGTGATCCAGCCCGACATCTACC
  CAGGGAATTGCTGGGCGTTCAAAGGTTCCCAGGGGTACCTGGTGGTGCGGTTGTCCATGAAGATCTACCC
  AACCACATTCACCATGGAACACATTCCAAAGACACTATCACCCACTGGTAACATCTCCAGTGCCCCCAAA
  GACTTTGCAGTCTATGGACTGGAAACGGAGTATCAAGAAGAGGGGCAGCCTCTGGGACGGTTCACCTATG
  ACCAGGAAGGAGACTCACTCCAGATGTTCCACACACTGGAAAGACCTGACCAAGCCTTCCAGATAGTAGA
  GCTCCGGGTCCTGTCCAACTGGGGCCACCCTGAGTACACTTGCCTCTACCGGTTCCGAGTCCACGGAGAG
  CCCATCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211159 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDFSRLHTYTPPQCVPENTGYTYALSSSYSSDALDFETEHKLEPVFDSPRMSRRSLRLVTTASYSSGDSQ
  AIDSHISTSRATPAKGRETRTVKQRRSASKPAFSINHLSGKGLSSSTSHDSSCSLRSATVLRHPVLDESL
  IREQTKVDHFWGLDDDGDLKGGNKAATQGNGELAAEVASSNGYTCRDCRMLSARTDALTAHSAIHGTTSR
  VYSRDRTLKPRGVSFYLDRTLWLAKSTSSSFASFIVQLFQVVLMKLNFETYKLKGYESRAYESQSYETKS
  HESEAHLGHCGRMTAGELSRVDGESLCDDCKGKKHLEIHTATHSQLPQPHRVAGAMGRLCIYTGDLLVQA
  LRRTRAAGWSVAEAVWSVLWLAVSAPGKAASGTFWWLGSGWYQFVTLISWLNVFLLTRCLRNICKVFVLL
  LPLLLLLGAGVSLWGQGNFFSLLPVLNWTAMQPTQRVDDSKGMHRPGPLPPSPPPKVDHKASQWPQESDM
  GQKVASLSAQCHNHDERLAELTVLLQKLQIRVDQVDDGREGLSLWVKNVVGQHLQEMGTIEPPDAKTDFM
  TFHHDHEVRLSNLEDVLRKLTEKSEAIQKELEETKLKAGSRDEEQPLLDRVQHLELELNLLKSQLSDWQH
  LKTSCEQAGARIQETVQLMFSEDQQGGSLEWLLEKLSSRFVSKDELQVLLHDLELKLLQNITHHITVTGQ
  APTSEAIVSAVNQAGISGITEAQAHIIVNNALKLYSQDKTGMVDFALESGGGSILSTRCSETYETKTALL
  SLFGVPLWYFSQSPRVVIQPDIYPGNCWAFKGSQGYLVVRLSMKIYPTTFTMEHIPKTLSPTGNISSAPK
  DFAVYGLETEYQEEGQPLGRFTYDQEGDSLQMFHTLERPDQAFQIVELRVLSNWGHPEYTCLYRFRVHGE
  PIQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024451
• ORF Size: 2742 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024451.2, NP_077771.1
• RefSeq Size: 4133 bp
• RefSeq ORF: 2742 bp
• Locus ID: 77053
• UniProt ID: Q9D666
• Cytogenetics: 5 G2
• MW: 102 kDa
• Gene Summary: As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton (PubMed:20711465, PubMed:16380439, PubMed:24062341, PubMed:25892231, PubMed:26842404). The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning (PubMed:19874786). Required for interkinetic nuclear migration (INM) and essential for nucleokinesis and centrosome-nucleus coupling during radial neuronal migration in the cerebral cortex and during glial migration (PubMed:19874786). Involved in telomere attachment to nuclear envelope in the prophase of meiosis implicating a SUN1/2:KASH5 LINC complex in which SUN1 and SUN2 seem to act at least partial redundantly (PubMed:17543860, PubMed:19211677, PubMed:19509342, PubMed:24062341, PubMed:25892231, PubMed:26842404). Required for gametogenesis and involved in selective gene expression of coding and non-coding RNAs needed for gametogenesis (PubMed:17543860). Helps to define the distribution of nuclear pore complexes (NPCs) (PubMed:17724119). Required for efficient localization of SYNE4 in the nuclear envelope (PubMed:23348741). May be involved in nuclear remodeling during sperm head formation in spermatogenenis (PubMed:20711465). May play a role in DNA repair by suppressing non-homologous end joining repair to facilitate the repair of DNA cross-links (By similarity).[UniProtKB/Swiss-Prot Function]