Eif3c (NM_146200) Mouse Tagged ORF Clone

CAT#: MR211151

Eif3c (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 3, subunit C (Eif3c)

Product Images

Circular map for MR211151

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Eif3c
• Synonyms: 110kDa; 3230401O13Rik; Eif3s8; NIPIL(A3); NipilA3; Xs; Xsl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211151 representing NM_146200
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCGATTCTTCACCACCGGCTCGGACAGTGAGTCGGAGTCGTCCCTGTCCGGGGAGGAGCTCGTCA
  CCAAACCGGTCTCCGGGAACTACGGCAAACAGCCTTTGTTACTGAGCGAGGATGAGGAGGATACAAAGAG
  AGTTGTCCGAAGCGCCAAGGACAAGAGGTTTGAGGAGCTGACCAACCTCATACGAACCATCCGTAATGCC
  ATGAAGATTCGAGATGTCACCAAGTGTCTAGAAGAGTTTGAGCTCCTGGGAAAGGCCTATGGGAAAGCCA
  AGAGTATTGTGGATAAGGAAGGTGTCCCCCGATTCTACATCCGAATACTGGCTGACCTGGAGGACTATCT
  TAATGAGCTTTGGGAAGATAAAGAAGGAAAGAAGAAGATGAATAAAAACAACGCCAAGGCTCTGAGTACC
  TTGCGCCAGAAGATCCGGAAATACAACCGAGATTTCGAGTCACACATCACCAACTACAAGCAGAACCCTG
  AGCAGTCTGCAGATGAAGATGCAGAAAAGAATGAGGAAGACTCCGAAGGCTCTTCAGATGAGGATGAGGA
  CGAGGACGGAGTCGGTAATACGACTTTCTTGAAGAAGAAGCAAGAATCTTCTGGAGAGAGTCGGAAGTTC
  CACAAAAAGATGGAAGATGATGATGAGGACTCTGAAGATTCTGAAGATGAAGAATGGGACACCAGCTCCA
  CATCTTCTGATTCAGACTCTGAGGAAGAAGAAGGAAAACAAACTGTTCTGGCCTCAAAGTTCCTTAAAAA
  GGCACCCACTACAGAGGAGGACAAAAAAGCAGCTGAAAAGAAACGGGAAGACAAAGCCAAGAAGAAGCAC
  GATAGGAAGTCTAAGCGCCTGGATGAGGAAGAGGAGGACAATGAGGGCGGGGAGTGGGAACGGGTCCGAG
  GTGGCGTACCACTTGTTAAGGAAAAGCCAAAAATGTTTGCCAAGGGAACTGAGATCACTCATGCTGTTGT
  CATCAAGAAACTGAATGAGATTCTACAGGTCAGAGGCAAGAAGGGAACAGACCGTGCCACTCAGATTGAA
  TTGCTCCAGCTGCTGGTTCAGATTGCTGCTGAAAACAACCTGGGGGTGGGCGTCATTGTCAAGATCAAGT
  TCAATATCATTGCCTCTCTCTACGATTACAACCCTAACCTGGCCACGTACATGAAGCCAGAGATGTGGCA
  GATGTGCCTAGACTGCATCAATGAACTGATGGATACGTTGGTTGCACATTCCAACATCTTTGTTGGAGAG
  AACATTTTGGAAGAGAGTGAGAACTTACACAACTTTGATCAGCCACTGCGTGTACGAGGCTGCATCCTAA
  CTTTGGTGGAGCGAATGGATGAAGAATTTACCAAAATAATGCAAAATACTGATCCTCACTCCCAAGAGTA
  TGTGGAGCACCTGAAGGATGAGGCACAAGTGTGTGCCATCATTGAGCGAGTGCAGCGCTACCTGGAGGAG
  AAAGGTACCACTGAGGAGATCTGCCAGATCTACTTAAGGCGCATCCTGCACACGTACTACAAGTTTGACT
  ACAAGGCCCATCAGCGGCAGCTTACTCCTCCTGAAGGATCCTCAAAGTCTGAGCAAGACCAGGCAGAAAA
  TGAGGGTGAGGACTCAGCTGTGCTAATGGAAAGACTGTGCAAGTACATCTATGCCAAGGACCGTACAGAC
  CGGATCCGTACCTGTGCCATCCTCTGCCATATCTACCATCATGCGCTCCACTCCCGCTGGTATCAGGCCC
  GTGACCTCATGCTCATGAGCCACCTACAGGACAACATTCAGCACGCAGACCCGCCGGTGCAGATCCTGTA
  TAACCGTACTATGGTGCAACTGGGCATCTGTGCTTTCCGCCAAGGCCTGACAAAGGATGCACACAATGCA
  CTTCTGGATATTCAGTCAAGTGGTCGTGCCAAGGAGCTTCTAGGTCAGGGTCTGCTGCTGCGCAGCCTGC
  AGGAGCGAAATCAGGAACAGGAAAAGGTAGAGCGACGCCGGCAGGTGCCCTTTCACCTGCACATCAACCT
  GGAGCTGCTGGAGTGTGTCTATCTGGTGTCAGCTATGCTCCTGGAGATCCCCTACATGGCTGCCCATGAG
  AGCGATGCCCGCCGACGCATGATCAGCAAGCAGTTCCACCACCAACTGCGGGTGGGCGAGCGGCAGCCCC
  TGCTAGGTCCTCCCGAGTCAATGAGGGAGCATGTGGTCGCTGCCTCCAAGGCCATGAAGATGGGCGACTG
  GAAGACCTGCCACAGTTTCATCATTAATGAAAAGATGAATGGGAAAGTGTGGGACCTTTTCCCTGAGGCT
  GACAAAGTTCGCACCATGCTAGTTCGGAAGATCCAGGAAGAGTCTCTGAGGACCTACCTTTTTACCTACA
  GCAGTGTCTATGACTCAATCAGTATGGAGACACTATCAGATATGTTTGAGCTGGATCTACCCACTGTTCA
  CTCCATCATCAGCAAGATGATCATTAACGAAGAATTGATGGCTTCCCTGGACCAGCCGACACAGACTGTG
  GTGATGCACCGTACTGAGCCCACTGCCCAGCAGAACTTGGCTCTGCAGCTGGCTGAGAAGCTTGGCAGCC
  TAGTGGAGAATAATGAACGGGTGTTTGACCATAAGCAGGGAACCTATGGTGGCTATTTCCGAGACCAAAA
  GGATGGCTACCGAAAAAATGAAGGCTATATGCGCCGTGGTGGCTACCGCCAGCAGCAGTCTCAGACAGCC
  TAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211151 representing NM_146200
  Red=Cloning site Green=Tags(s)
  
  MSRFFTTGSDSESESSLSGEELVTKPVSGNYGKQPLLLSEDEEDTKRVVRSAKDKRFEELTNLIRTIRNA
  MKIRDVTKCLEEFELLGKAYGKAKSIVDKEGVPRFYIRILADLEDYLNELWEDKEGKKKMNKNNAKALST
  LRQKIRKYNRDFESHITNYKQNPEQSADEDAEKNEEDSEGSSDEDEDEDGVGNTTFLKKKQESSGESRKF
  HKKMEDDDEDSEDSEDEEWDTSSTSSDSDSEEEEGKQTVLASKFLKKAPTTEEDKKAAEKKREDKAKKKH
  DRKSKRLDEEEEDNEGGEWERVRGGVPLVKEKPKMFAKGTEITHAVVIKKLNEILQVRGKKGTDRATQIE
  LLQLLVQIAAENNLGVGVIVKIKFNIIASLYDYNPNLATYMKPEMWQMCLDCINELMDTLVAHSNIFVGE
  NILEESENLHNFDQPLRVRGCILTLVERMDEEFTKIMQNTDPHSQEYVEHLKDEAQVCAIIERVQRYLEE
  KGTTEEICQIYLRRILHTYYKFDYKAHQRQLTPPEGSSKSEQDQAENEGEDSAVLMERLCKYIYAKDRTD
  RIRTCAILCHIYHHALHSRWYQARDLMLMSHLQDNIQHADPPVQILYNRTMVQLGICAFRQGLTKDAHNA
  LLDIQSSGRAKELLGQGLLLRSLQERNQEQEKVERRRQVPFHLHINLELLECVYLVSAMLLEIPYMAAHE
  SDARRRMISKQFHHQLRVGERQPLLGPPESMREHVVAASKAMKMGDWKTCHSFIINEKMNGKVWDLFPEA
  DKVRTMLVRKIQEESLRTYLFTYSSVYDSISMETLSDMFELDLPTVHSIISKMIINEELMASLDQPTQTV
  VMHRTEPTAQQNLALQLAEKLGSLVENNERVFDHKQGTYGGYFRDQKDGYRKNEGYMRRGGYRQQQSQTA
  Y
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_146200
• ORF Size: 2733 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146200.1, NP_666312.1
• RefSeq Size: 2896 bp
• RefSeq ORF: 2736 bp
• Locus ID: 56347
• UniProt ID: Q8R1B4
• Cytogenetics: 7 F3
• MW: 105.5 kDa
• Gene Summary: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.[UniProtKB/Swiss-Prot Function]