Dag1 (NM_010017) Mouse Tagged ORF Clone

CAT#: MR211082

Dag1 (Myc-DDK-tagged) - Mouse dystroglycan 1 (Dag1)

Product Images

Circular map for MR211082

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dag1
• Synonyms: D9Wsu13; D9Wsu13e; DG; Dp71; Dp427
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211082 representing NM_010017
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGTGGACAACTGGCTACTGCACCCCCTCTGGGGACAGACCTTTCTCCTCCTCCTGTCTGTGGCTG
  TGGCTCAGGCCCACTGGCCCAGTGAACCCTCAGAGGCTGTGAGGGACTGGAAGAACCAGCTTGAGGCGTC
  CATGCACTCAGTTCTCTCCGACTTCCAGGAGGCTGTTCCCACCGTGGTTGGCATTCCAGACGGTACGGCT
  GTTGTCGGGCGCTCATTTCGAGTGAGCATTCCAACGGATTTAATTGCCTCCAGTGGGGAGATCATCAAGG
  TGTCTGCAGCAGGGAAGGAGGCCTTACCGTCTTGGCTACACTGGGACCCACACAGTCATATTTTGGAAGG
  CCTTCCTCTTGACACTGATAAAGGTGTGCATTACATCTCAGTGAGTGCTGCACGCCTGGGAGCCAATGGA
  AGCCACGTCCCCCAGACTTCCAGTGTGTTCTCTATCGAGGTCTACCCTGAAGACCACAATGAGCCACAGT
  CTGTACGGGCAGCCTCATCAGACCCTGGTGAGGTAGTGCCATCTGCCTGTGCTGCTGATGAGCCAGTGAC
  TGTCCTTACAGTGATTCTGGATGCTGACCTCACCAAGATGACCCCAAAGCAAAGGATCGATCTGTTGAAC
  AGAATGCAGAGCTTCTCAGAAGTAGAACTTCACAACATGAAGTTGGTGCCTGTAGTGAATAATAGACTAT
  TTGACATGTCGGCCTTCATGGCTGGCCCAGGAAATGCAAAGAAAGTGGTAGAGAATGGGGCTCTCCTGTC
  CTGGAAACTAGGCTGCTCCTTGAACCAGAATAGCGTCCCTGACATCCGTGGTGTAGAAACCCCTGCTAGG
  GAGGGTGCTATGTCTGCCCAACTTGGTTATCCTGTGGTGGGTTGGCACATTGCCAATAAGAAGCCCACTC
  TCCCCAAACGACTCCGGAGGCAGATCCACGCCACACCTACACCTGTTACTGCCATTGGACCCCCAACCAC
  GGCCATTCAGGAGCCACCATCGCGGATAGTGCCTACGCCTACATCTCCAGCCATTGCACCTCCAACAGAG
  ACCATGGCTCCTCCTGTCAGGGATCCTGTTCCAGGGAAGCCCACGGTCACCATTCGGACGCGAGGTGCCA
  TTATTCAGACCCCAACTCTGGGCCCTATCCAGCCTACTCGGGTGTCAGAAGCTGGTACCACGGTTCCTGG
  CCAGATTCGCCCAACACTGACAATTCCTGGCTATGTAGAGCCCACAGCCGTTATTACTCCTCCAACAACT
  ACCACAAAGAAGCCACGAGTGTCCACGCCAAAGCCAGCAACGCCTTCAACTGATTCGTCAACTACCACAA
  CTCGGAGGCCAACCAAAAAACCACGGACACCCCGACCAGTGCCCCGAGTCACCACCAAAGCACCCATCAC
  CAGGTTGGAGACAGCTTCCCCACCCACTCGAATCCGTACTACCACCAGTGGAGTGCCCCGTGGGGGAGAA
  CCTAACCAGCGGCCAGAGCTCAAGAATCACATTGACAGGGTAGATGCCTGGGTGGGAACCTATTTTGAGG
  TAAAGATTCCATCAGACACCTTCTATGACAATGAGGATACCACTACCGACAAGCTCAAGCTGACCCTGAA
  GCTTCGAGAGCAGCAGTTAGTAGGTGAGAAATCGTGGGTTCAGTTTAACAGCAACAGCCAGCTCATGTAT
  GGCCTGCCTGACAGCAGCCATGTGGGAAAACATGAGTATTTTATGCATGCCACAGACAAAGGGGGCCTCT
  CCGCTGTGGATGCCTTCGAGATCCATGTTCACAAGCGCCCACAAGGGGACAAGGCTCCTGCACGGTTCAA
  GGCCAGGCTTGCAGGGGATCCAGCACCGGTGGTGAATGACATTCACAAGAAAATTGCTTTGGTAAAGAAG
  CTAGCTTTTGCTTTTGGGGATCGAAACTGCAGCTCCATCACCCTTCAGAACATCACTCGGGGCTCTATCG
  TGGTGGAATGGACCAACAACACTCTGCCCCTGGAGCCCTGCCCCAAGGAGCAGATCATAGGGCTGAGCCG
  CAGGATTGCTGATGAAAATGGGAAGCCTCGTCCTGCCTTCTCCAATGCTCTGGAGCCTGACTTTAAGGCT
  CTGAGTATTGCTGTGACGGGCTCTGGCAGTTGTCGGCACCTCCAGTTTATCCCTGTGGCACCACCCTCTC
  CTGGAAGCTCAGCTGCACCAGCCACAGAGGTTCCAGACAGGGACCCCGAGAAGAGCAGTGAGGACGATGT
  TTACCTGCACACCGTTATCCCAGCCGTGGTGGTCGCGGCCATCCTGCTCATTGCTGGAATCATTGCTATG
  ATCTGCTATCGCAAGAAGAGGAAGGGCAAGCTGACCCTTGAGGACCAGGCCACCTTTATTAAGAAGGGGG
  TGCCTATCATCTTTGCGGATGAGCTGGATGACTCTAAGCCCCCGCCCTCTTCCAGCATGCCGCTCATCTT
  GCAGGAAGAGAAGGCTCCCCTCCCACCTCCTGAGTACCCCAACCAGAGTATGCCCGAGACCACTCCTCTG
  AACCAGGACACTGTGGGAGAGTACACACCCCTGCGGGATGAGGATCCTAACGCACCTCCCTATCAGCCAC
  CCCCACCCTTCACGGCTCCCATGGAGGGCAAGGGCTCCCGTCCCAAGAACATGACCCCATACCGATCACC
  CCCTCCGTATGTTCCCCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211082 representing NM_010017
  Red=Cloning site Green=Tags(s)
  
  MSVDNWLLHPLWGQTFLLLLSVAVAQAHWPSEPSEAVRDWKNQLEASMHSVLSDFQEAVPTVVGIPDGTA
  VVGRSFRVSIPTDLIASSGEIIKVSAAGKEALPSWLHWDPHSHILEGLPLDTDKGVHYISVSAARLGANG
  SHVPQTSSVFSIEVYPEDHNEPQSVRAASSDPGEVVPSACAADEPVTVLTVILDADLTKMTPKQRIDLLN
  RMQSFSEVELHNMKLVPVVNNRLFDMSAFMAGPGNAKKVVENGALLSWKLGCSLNQNSVPDIRGVETPAR
  EGAMSAQLGYPVVGWHIANKKPTLPKRLRRQIHATPTPVTAIGPPTTAIQEPPSRIVPTPTSPAIAPPTE
  TMAPPVRDPVPGKPTVTIRTRGAIIQTPTLGPIQPTRVSEAGTTVPGQIRPTLTIPGYVEPTAVITPPTT
  TTKKPRVSTPKPATPSTDSSTTTTRRPTKKPRTPRPVPRVTTKAPITRLETASPPTRIRTTTSGVPRGGE
  PNQRPELKNHIDRVDAWVGTYFEVKIPSDTFYDNEDTTTDKLKLTLKLREQQLVGEKSWVQFNSNSQLMY
  GLPDSSHVGKHEYFMHATDKGGLSAVDAFEIHVHKRPQGDKAPARFKARLAGDPAPVVNDIHKKIALVKK
  LAFAFGDRNCSSITLQNITRGSIVVEWTNNTLPLEPCPKEQIIGLSRRIADENGKPRPAFSNALEPDFKA
  LSIAVTGSGSCRHLQFIPVAPPSPGSSAAPATEVPDRDPEKSSEDDVYLHTVIPAVVVAAILLIAGIIAM
  ICYRKKRKGKLTLEDQATFIKKGVPIIFADELDDSKPPPSSSMPLILQEEKAPLPPPEYPNQSMPETTPL
  NQDTVGEYTPLRDEDPNAPPYQPPPPFTAPMEGKGSRPKNMTPYRSPPPYVPP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010017
• ORF Size: 2679 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010017.4, NP_034147.1
• RefSeq Size: 5591 bp
• RefSeq ORF: 2682 bp
• Locus ID: 13138
• UniProt ID: Q62165
• Cytogenetics: 9 59.08 cM
• MW: 97.4 kDa
• Gene Summary: This gene encodes dystroglycan, a central component of dystrophin-glycoprotein complex that links the extracellular matrix and the cytoskeleton in the skeletal muscle. The encoded preproprotein undergoes O- and N-glycosylation, and proteolytic processing to generate alpha and beta subunits. A complete lack of the encoded protein in mice results in embryonic lethality due to the disorganization of Reichert's membrane. Chimeric mice deficient in the encoded protein overcome embryonic lethality but develop a progressive muscular dystrophy. Alternative splicing results in multiple transcript variants, all encoding the same protein. [provided by RefSeq, Nov 2015]