Dsc2 (NM_013505) Mouse Tagged ORF Clone

CAT#: MR210942

  • TrueORF®

Dsc2 (Myc-DDK-tagged) - Mouse desmocollin 2 (Dsc2)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro


  "NM_013505" in other vectors (4)


Interest in protein/lysate? Submit request here!

Reconstitution Protocol

USD 776.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

USD 650.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 160.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

USD 198.00

Other products for "Dsc2"

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Dsc2
Synonyms AW228162; Dsc
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR210942 representing NM_013505
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCTGTGGGATCTATGCGCTCCGGGAGCCCTGCCTTCGGCCTGGGACACCTGTTGACCCTTGCGA
TCCTTGCACTTGCCTCTGATGCCTGTAAAGAAGTCGTCCTCCAGGTCCCCTCTGAACTACCTGCCGAGAA
ATTTGTTGGCAGAGTGAACCTGATGGACTGCCTTAAATCAGCAGACATAGTTCATCTGAGTGATCCTGAC
TTCCAAGTCTTAGAAGATGGTTCTGTGTACACAACCAGTTCTGTTGTTTTGTCCTCGGGGCAAAGAAGCT
TTACTATATGGCTTTTTAGCACAGACAGCCAAGAAGAAAGGGAGATATCTGTCCATTTAGAGGGCCCAGT
AGAGGTACTAAATAAAAGACCGCATACAGAGAAGGTTCTCAGCCGTGCCAAGAGAAGATGGGCTCCTATC
CCTTGTTCCATGCTAGAGAATTCATTGGGTCCCTTCCCACTTTTCCTTCAACAGATCCAGTCTGACACAG
CTCAAAACTATACCATATACTATTCCATCAGAGGTCCAGGAGTTGATAAAGAACCTCTAAACTTATTTTA
TGTGGAAAGAGATACTGGAAACCTGTATTGCACTGGTCGTGTAGATCGTGAGCAGTATGAGTCCTTTGAG
CTGACTGCATTTGCAACAACTCCAGATGGGTATACGCCAGAGTACCCACTGCCGCTCCTCATTAAAATAG
AGGACGAGAACGATAATTACCCGATCTTTACACAGAAACTGTACAGCTTCACAGTTCAGGAAAATAGTCG
CATTGGTTCTATTGTGGGGGAGGTCTGTGCAACCGACCTGGATGAGCCAGACACAATGCACACTCGCCTG
AGGTACTCCATCCTGGAGCAGTCGCCTTCTCCACCCATGCTATTTACTATGCATCCCAGCACTGGTGTGA
TCACTACCACGTCGGCTCAGCTGGACAGAGAGTTAATTGACAAGTACCAGTTGCTAATAAAAGTCCAGGA
TATGGATGGCCAGTATTTTGGTTTGCACACAACAGCAAAGTGCATCATTACTATTGAAGATGTGAATGAC
AACCTGCCCACGTTCACTCGCACTACGTATGTGACATCAGTGGAAGAAAATACAGTTAATGTGGAAATCT
TGCGACTTACTGTTCAGGATAAAGATTTAGTTAACTCACCTAATTGGAGAGCTAATTACACCATTTTGAA
GGGCAATGAAAATGGAAATTTTAAAATCGTAACAGATCCTAAAACCAATGAAGGCATTCTGTGTGTGATT
AAGCCTCTGGACTATGAAGAACGGCAACAGGTGACCCTGCAAATTGGAGTAGTTAATGAAGCTCCATACA
CTAGAGAGGCTAGCTCAAAGTCACCCATGAGCACAGCCACAGTGACTGTCACCGTGACAAATCAGGATGA
GGGCCCCGAGTGTATCCCTCCAATGCAAACTGTGAGGATTCAAGAAAATGTACCAGTTGGGACTAGAAAT
GATGGATATAAGGCATATGACCCAGAGACCAGAAGCAGCAGTGGCATAAGGTACAGAAAGTTAAGTGACC
CAAGAGGGTGGGTCACTGTTAATGAAGATTCAGGATCGATCACTATTTTCCGAGCGTTGGATCGAGAGGC
TGAGACTGTCAGAAATGGCATTTACAATATTACAGTCCTTGCGTTAGATGCAGATGGGAGAAGCTGTACT
GGAACTCTGGGAATCATACTTGAAGATGTGAATGATAATGGCCCATTCATACCCAAGCAGACAGTGGTGA
TATGCAAGGCTACCATGTCCTCTGCTGAAATTGTTGCAGTTGATCTCGATGATCCAGTGAATGGCCCACC
ATTTGATTTCAGTTTGGAGAGCTCTGATTCGGAAGTTCAGAGGATGTGGAGGCTAACAAGAATCAATGAT
ACAGCAGCTCGTCTTTCCTATCAGAACGACCCTTCATTTGGATCCTATGCAGTTCCCATTCGAGTTACAG
ACAGACTTGGTCTGTCCTCAGTAACCACATTGAATGTCCTTGTATGTGACTGCATTACTGAAAGTGACTG
CACACTCCGCTCTGGAGAAAGGACTGGCTATGCTGATGTGAGACTTGGACCATGGGCCATCCTTGCTATA
CTGTTGGGCATAGCCTTGCTATTTTGTATCCTGTTTACATTAGTCTGTAGTGTTTCCCGGGCATCAAAAC
AACAGAAAATTCTTCCTGATGACTTAGCTCAACAGAACCTAATTGTATCAAACACCGAGGCTCCTGGAGA
TGACAAAGTTTATTCCACAAACGGCTTGACAACTCAAACTATGGGAGCTTCTGGGCAGACAGCTTTCACC
ACCATGGGAACTGGAGTCAAAAGTGGAGGGCAAGAGACCATTGAGATGGTAAAAGGAGGGCAGCAGACTT
TGGACTCACGCAGAGGGGCTGGCTATCATCACCATACCCTGGATCCTTGCAGGGGAGGACATGTGGAGGT
GGACAACTACAGACACACTTACTCAGAGTGGTACAATTTCATTCAGCCCCGACTTGGTGATGAAACCATT
AGAGGACACACTCTGATTAAAAAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR210942 representing NM_013505
Red=Cloning site Green=Tags(s)

MAAVGSMRSGSPAFGLGHLLTLAILALASDACKEVVLQVPSELPAEKFVGRVNLMDCLKSADIVHLSDPD
FQVLEDGSVYTTSSVVLSSGQRSFTIWLFSTDSQEEREISVHLEGPVEVLNKRPHTEKVLSRAKRRWAPI
PCSMLENSLGPFPLFLQQIQSDTAQNYTIYYSIRGPGVDKEPLNLFYVERDTGNLYCTGRVDREQYESFE
LTAFATTPDGYTPEYPLPLLIKIEDENDNYPIFTQKLYSFTVQENSRIGSIVGEVCATDLDEPDTMHTRL
RYSILEQSPSPPMLFTMHPSTGVITTTSAQLDRELIDKYQLLIKVQDMDGQYFGLHTTAKCIITIEDVND
NLPTFTRTTYVTSVEENTVNVEILRLTVQDKDLVNSPNWRANYTILKGNENGNFKIVTDPKTNEGILCVI
KPLDYEERQQVTLQIGVVNEAPYTREASSKSPMSTATVTVTVTNQDEGPECIPPMQTVRIQENVPVGTRN
DGYKAYDPETRSSSGIRYRKLSDPRGWVTVNEDSGSITIFRALDREAETVRNGIYNITVLALDADGRSCT
GTLGIILEDVNDNGPFIPKQTVVICKATMSSAEIVAVDLDDPVNGPPFDFSLESSDSEVQRMWRLTRIND
TAARLSYQNDPSFGSYAVPIRVTDRLGLSSVTTLNVLVCDCITESDCTLRSGERTGYADVRLGPWAILAI
LLGIALLFCILFTLVCSVSRASKQQKILPDDLAQQNLIVSNTEAPGDDKVYSTNGLTTQTMGASGQTAFT
TMGTGVKSGGQETIEMVKGGQQTLDSRRGAGYHHHTLDPCRGGHVEVDNYRHTYSEWYNFIQPRLGDETI
RGHTLIKN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_013505
ORF Size 2544 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_013505.4, NP_038533.1
RefSeq Size 4416 bp
RefSeq ORF 2547 bp
Locus ID 13506
UniProt ID P55292
Cytogenetics 18 11.14 cM
MW 94.4 kDa
Gene Summary This gene encodes a member of the desmocollin protein subfamily. Desmocollins are cadherin-like transmembrane glycoproteins that are major components of the desmosome. Desmosomes are cell-cell junctions that help resist shearing forces and are found in high concentrations in cells subject to mechanical stress. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

Other Versions

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.