Punc (BC053057) Mouse Tagged ORF Clone

CAT#: MR210783

Punc (Myc-DDK-tagged) - Mouse putative neuronal cell adhesion molecule (cDNA clone MGC:62345 IMAGE:5698028)

Product Images

Circular map for MR210783

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Punc
• Synonyms: 2810401C09Rik; AI851425; Punc; WI-14920
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210783 representing BC053057
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAGCCGCGCACCGCGTCCCCGCGCCGCCTGCCCGCGCTGCGCCGGCCCGGGTTTCTGCCGCCGT
  TGCTGCCGCCGCCGCCGCCGCCGCTGCTGTTGCTGCTGCTGCTGTTGCCGCTGCCTGCGCCAAGCCTGGG
  TCTGGGCCATTCTGCTGAACTGGCATTTTCTGTGGAGCCAAATGATGATATTGCCAACCCTGGGCAGCCC
  ATAGTGCTGGGCTGCAAAGTGGAGGGCACCCCTCCAGTGCAAGTCTCCTGGAGGAAGAATGGAGCAGAGC
  TACCAGAGGGCACCCACACCACTCTGCTGGCCAATGGCTCCTTGCTGATCCACCACTTCAGGCTGGAGCA
  AGGAGGTAGCCCTTCAGATGAGGGTGACTATGAATGTGTGGCTCAGAACCGCTTTGGGCTGCTGGTCAGT
  CGGAAGGCTCGTCTCCAGGCTGCAACTATGTCCGACTTCCACGTACACCCCCAGGCCGTCACGGGTGAGG
  AGGGTGGTGTGGCCCGCTTCCAGTGCCAAATCCATGGGCTTCCCAAGCCCCTGATCACATGGGAGAAGAA
  CAGAGTTCCCATTGACACGGACGATGAGAGGTACACACTACTGCCCAAGGGAGTCCTGCAGATAACAGGA
  CTTCGGGCTGAGGACAGCGGCATCTTCCACTGTGTGGCCTCAAACATTGCTAGTGTGCGGGTCAGCCACG
  GGGCCAGGCTCACTGTGTCAGGCTCGGGCTCAGGGACCTACAAGGAGCCCACCATTCTTGTGGGGCCTGA
  GAACCTCACCCTGACAGTGCACCAGACTGCGGTGCTGGAGTGTGTCGCCACGGGCAACCCGCGCCCCATT
  GTGTCCTGGAGCCGCTTGGATGGCCGCCCCATTGGGGTGGAGGGCATCCAGGTGCTGGGCACAGGAAACC
  TTATCATCTCAGATGTGACTGTCCAGCATTCAGGTGTCTATGTCTGTGCAGCCAACAGGCCTGGCACCCG
  GGTGAGAAGGACGGCCCAGGGTCGGCTGGTGGTACAAGCTCCAGCAGAGTTTGTTCAGCATCCCCAGTCC
  ATCTCCAGGCCTGCTGGGACCACAGCCATGTTCACCTGCCAAGCGCAGGGGGAGCCACCGCCTCATGTCA
  CATGGTTGAAGAATGGACAAGTGCTGGGGGCAGGAGGCCATGTCAGGCTCAAGAACAATAACAGCACACT
  GAGCATTTCTGGAGTCGGACCTGAAGATGAGGCCATCTACCAATGTGTGGCTGAGAACATTGCTGGCTCG
  TCGCAAGCCAGCGCCAGGCTGACCGTGCTGTGGGCTGAGGGGTTGCCAGGCCCCCCACGCAATGTCCGAG
  CTGTCTCTGTGTCCTCCACTGAAGTTCGAGTGTCCTGGAGTGAGCCTCTGGCCCACACCAAAGAGATCAT
  TGGCTATGTCTTGCACATCAGGAAGGCTGCCGACTCACCCAAGCTAGAGTACCAGGAAGCTGTCAGCAAG
  AGCACCTTTCAGCACCTGGTGAGAGACCTTGAGCCCTCCACTGCCTACAGCTTCTACATCAAAGCCTACA
  CACCACGAGGGGCCAGTTTAGCCTCTGTCCCCACCCTGGCCAGCACCCTGGGTGAAGCCCCTGTCCCACC
  CCCATTATCTGTGCGGTTGCTGGGCAGCTCCTCTCTACAACTGCTATGGAAGCCCTGGCCACGGCTGGCC
  CAACACAATGGAGGCTTCAAGCTGTTTTACCGCCCAGTCAGTGCAACCTCCTTCACTGGCCCCATCTTGC
  TCCCTGGGACTGTCTCCTCTTACAACCTCAGCCAGCTTGACCCCAGCACCGTGTACGAGGTGAAGCTCCT
  GGCCTACAACCAGCATGGAGATGGAAACGCCACGG[AT]CCGATTTGTGTCTCTGAAGGGAGCCTCTGAG
  AGGACAGCCCTGACTCCCCCATGTGACTGCCGGAAGGAAGATGTCACCAACCATACGTCCACTACAGGCA
  TTGTTATCGGCATCCACATCGGAGTCACCTGCATCATCTTCTGCGTCCTCTTTCTTCTGTTTGGCCAGAG
  GGGCAGGGTCCTCCTGTGTAAGGATGTGGAAAACCAGCTCTCCCCTCCCCAGGGTCCCCGGAGCCAGAGG
  GACCCTGGTATCCTGGCCCTGAATGGACTGAGCCGAGGAGAAGGGGGCCAGCTGAGCCGAGATGAAAAGC
  CGGTGGACGCCAAGGAGTTGGAACAGCTGTTTCCCACAGCTGGGTCAGCTGCACAGCCAGGCTCCACACC
  CACGGATCCTGCAGCCCCTGCTCCATGTGAGGAGACCCAGCTCTCCATGGTTCAGCTTCAAGGCTTCAAC
  CTTGTGGCAGGGAGGACAACAGAGGCCACGTCCCCCTGTGCAGGGCCTGGGCCTGTCCCAGCACCCCAGG
  ATATAGGCCCTGTCCCCCTCAGTGAAGGACAGACCCAGCCTCCAGCAGTCGCAGCACCCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210783 representing BC053057
  Red=Cloning site Green=Tags(s)
  
  MAEPRTASPRRLPALRRPGFLPPLLPPPPPPLLLLLLLLPLPAPSLGLGHSAELAFSVEPNDDIANPGQP
  IVLGCKVEGTPPVQVSWRKNGAELPEGTHTTLLANGSLLIHHFRLEQGGSPSDEGDYECVAQNRFGLLVS
  RKARLQAATMSDFHVHPQAVTGEEGGVARFQCQIHGLPKPLITWEKNRVPIDTDDERYTLLPKGVLQITG
  LRAEDSGIFHCVASNIASVRVSHGARLTVSGSGSGTYKEPTILVGPENLTLTVHQTAVLECVATGNPRPI
  VSWSRLDGRPIGVEGIQVLGTGNLIISDVTVQHSGVYVCAANRPGTRVRRTAQGRLVVQAPAEFVQHPQS
  ISRPAGTTAMFTCQAQGEPPPHVTWLKNGQVLGAGGHVRLKNNNSTLSISGVGPEDEAIYQCVAENIAGS
  SQASARLTVLWAEGLPGPPRNVRAVSVSSTEVRVSWSEPLAHTKEIIGYVLHIRKAADSPKLEYQEAVSK
  STFQHLVRDLEPSTAYSFYIKAYTPRGASLASVPTLASTLGEAPVPPPLSVRLLGSSSLQLLWKPWPRLA
  QHNGGFKLFYRPVSATSFTGPILLPGTVSSYNLSQLDPSTVYEVKLLAYNQHGDGNATXXRFVSLKGASE
  RTALTPPCDCRKEDVTNHTSTTGIVIGIHIGVTCIIFCVLFLLFGQRGRVLLCKDVENQLSPPQGPRSQR
  DPGILALNGLSRGEGGQLSRDEKPVDAKELEQLFPTAGSAAQPGSTPTDPAAPAPCEETQLSMVQLQGFN
  LVAGRTTEATSPCAGPGPVPAPQDIGPVPLSEGQTQPPAVAAPQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC053057
• ORF Size: 2442 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC053057.1
• RefSeq Size: 3198 bp
• RefSeq ORF: 2441 bp
• Locus ID: 19289
• Cytogenetics: 9 35.13 cM
• MW: 117.3 kDa