Hsp90b1 (NM_011631) Mouse Tagged ORF Clone

CAT#: MR210732

Hsp90b1 (Myc-DDK-tagged) - Mouse heat shock protein 90, beta (Grp94), member 1 (Hsp90b1)

Product Images

Circular map for MR210732

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hsp90b1
• Synonyms: endoplasmin; ERp99; gp96; GRP94; TA-3; Targ2; Tra-1; Tra1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210732 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGTCCTGTGGGTGTTGGGCCTCTGCTGTGTCCTGCTGACCTTCGGGTTCGTCAGAGCTGATGATG
  AAGTCGACGTGGATGGCACAGTGGAAGAGGACCTGGGTAAAAGCCGAGAAGGCTCAAGGACAGATGATGA
  AGTTGTGCAGAGAGAGGAAGAAGCTATTCAGTTGGATGGGTTAAACGCATCACAGATAAGAGAACTTAGA
  GAAAAATCTGAAAAGTTCGCCTTCCAAGCTGAAGTGAACAGGATGATGAAACTTATCATCAATTCTTTGT
  ATAAAAATAAAGAGATTTTCCTGAGAGAACTGATTTCAAATGCTTCTGATGCTTTAGACAAGATAAGGCT
  CATCTCCCTAACTGATGAAAATGCACTCGCTGGAAATGAGGAGTTAACGGTCAAGATTAAGTGTGACAAA
  GAGAAAAACCTGCTGCATGTCACAGACACGGGTGTAGGAATGACTAGAGAGGAGTTGGTTAAAAATCTCG
  GCACCATAGCCAAATCTGGAACAAGCGAGTTTTTAAACAAAATGACAGAAGCTCAAGAAGATGGTCAGTC
  AACCTCTGAACTGATTGGCCAGTTTGGTGTCGGTTTTTATTCTGCCTTCCTTGTAGCAGATAAGGTCATT
  GTCACATCGAAACACAACAATGATACCCAGCACATCTGGGAATCAGACTCCAATGAATTCTCTGTAATTG
  CTGACCCAAGAGGAAACACACTAGGTCGTGGAACAACAATTACTCTTGTCTTAAAAGAAGAAGCATCTGA
  TTACCTTGAATTGGACACAATTAAAAATCTCGTCAGGAAGTACTCTCAGTTCATCAACTTTCCCATCTAC
  GTGTGGAGTAGCAAGACAGAGACTGTTGAGGAGCCCTTGGAAGAAGATGAAGCAGCAAAAGAAGAGAAAG
  AAGAATCTGATGATGAAGCTGCAGTAGAGGAGGAAGAAGAAGAAAAGAAACCAAAAACTAAGAAAGTTGA
  AAAAACTGTGTGGGATTGGGAACTTATGAATGATATCAAACCAATATGGCAGAGACCATCCAAAGAAGTA
  GAAGAAGACGAATACAAAGCTTTCTACAAATCATTTTCAAAGGAAAGTGATGACCCCATGGCTTATATCC
  ACTTCACTGCAGAAGGGGAGGTCACCTTCAAGTCGATTTTGTTTGTACCCACATCTGCACCTCGAGGTCT
  GTTTGATGAATATGGATCTAAGAAGAGTGATTATATTAAGCTGTATGTACGCCGCGTATTCATCACAGAT
  GACTTCCATGATATGATGCCCAAATACCTTAATTTTGTCAAAGGTGTTGTGGATTCCGATGATCTCCCCC
  TCAATGTTTCCCGTGAGACTCTTCAGCAACATAAATTGCTCAAGGTGATTAGGAAGAAGCTTGTCCGAAA
  AACTCTGGACATGATCAAGAAGATTGCTGATGAGAAGTATAACGACACTTTCTGGAAGGAGTTCGGCACG
  AATATCAAGCTTGGTGTGATTGAAGACCACTCAAATCGAACACGGCTTGCTAAACTTCTTAGGTTCCAGT
  CTTCTCACCATTCAACTGACATTACTAGTTTAGACCAGTATGTGGAAAGAATGAAGGAAAAACAGGACAA
  AATCTACTTCATGGCTGGGTCAAGCAGAAAGGAGGCGGAATCTTCTCCATTTGTTGAGAGACTTCTGAAG
  AAGGGCTATGAAGTCATTTATCTCACAGAGCCTGTGGATGAATACTGCATTCAGGCTCTTCCCGAGTTTG
  ATGGGAAGAGGTTTCAGAATGTTGCCAAAGAAGGGGTGAAGTTTGATGAGAGTGAGAAAACTAAAGAAAG
  TCGGGAAGCAACAGAGAAGGAGTTTGAACCTCTGCTCAACTGGATGAAAGATAAGGCCCTCAAGGACAAG
  ATAGAAAAGGCTGTGGTGTCGCAGCGCCTCACAGAGTCTCCCTGTGCTCTTGTGGCCAGTCAGTATGGAT
  GGTCTGGCAACATGGAGAGGATCATGAAGGCACAAGCATACCAGACGGGCAAGGACATCTCTACAAATTA
  CTATGCCAGTCAAAAGAAAACGTTCGAAATCAATCCTAGACACCCACTGATCAGAGACATGTTGCGGCGG
  ATTAAGGAAGATGAAGATGACAAGACAGTCATGGATCTTGCTGTAGTTTTGTTTGAAACGGCAACACTTC
  GGTCAGGATATCTTCTACCAGACACCAAGGCGTATGGAGATAGAATAGAAAGAATGCTTCGCCTCAGTTT
  AAACATTGACCCTGAAGCACAGGTGGAGGAAGAACCAGAAGAAGAGCCTGAAGACACCTCAGAAGAGGCA
  GAAGACTCAGAGCAGGATGAGGGAGAAGAGATGGATGCAGGGACAGAAGAAGAAGAGGAGGAAACAGAAA
  AGGAATCTACAGAGAAGGATGAATTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210732 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRVLWVLGLCCVLLTFGFVRADDEVDVDGTVEEDLGKSREGSRTDDEVVQREEEAIQLDGLNASQIRELR
  EKSEKFAFQAEVNRMMKLIINSLYKNKEIFLRELISNASDALDKIRLISLTDENALAGNEELTVKIKCDK
  EKNLLHVTDTGVGMTREELVKNLGTIAKSGTSEFLNKMTEAQEDGQSTSELIGQFGVGFYSAFLVADKVI
  VTSKHNNDTQHIWESDSNEFSVIADPRGNTLGRGTTITLVLKEEASDYLELDTIKNLVRKYSQFINFPIY
  VWSSKTETVEEPLEEDEAAKEEKEESDDEAAVEEEEEEKKPKTKKVEKTVWDWELMNDIKPIWQRPSKEV
  EEDEYKAFYKSFSKESDDPMAYIHFTAEGEVTFKSILFVPTSAPRGLFDEYGSKKSDYIKLYVRRVFITD
  DFHDMMPKYLNFVKGVVDSDDLPLNVSRETLQQHKLLKVIRKKLVRKTLDMIKKIADEKYNDTFWKEFGT
  NIKLGVIEDHSNRTRLAKLLRFQSSHHSTDITSLDQYVERMKEKQDKIYFMAGSSRKEAESSPFVERLLK
  KGYEVIYLTEPVDEYCIQALPEFDGKRFQNVAKEGVKFDESEKTKESREATEKEFEPLLNWMKDKALKDK
  IEKAVVSQRLTESPCALVASQYGWSGNMERIMKAQAYQTGKDISTNYYASQKKTFEINPRHPLIRDMLRR
  IKEDEDDKTVMDLAVVLFETATLRSGYLLPDTKAYGDRIERMLRLSLNIDPEAQVEEEPEEEPEDTSEEA
  EDSEQDEGEEMDAGTEEEEEETEKESTEKDEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011631
• ORF Size: 2409 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011631.1, NP_035761.1
• RefSeq Size: 2759 bp
• RefSeq ORF: 2409 bp
• Locus ID: 22027
• UniProt ID: P08113
• Cytogenetics: 10 43.05 cM
• MW: 92.5 kDa
• Gene Summary: Molecular chaperone that functions in the processing and transport of secreted proteins (PubMed:20865800). When associated with CNPY3, required for proper folding of Toll-like receptors (PubMed:20865800). Functions in endoplasmic reticulum associated degradation (ERAD) (By similarity). Has ATPase activity (PubMed:20865800).[UniProtKB/Swiss-Prot Function]