Itgb6 (NM_001159564) Mouse Tagged ORF Clone

CAT#: MR210654

Itgb6 (Myc-DDK-tagged) - Mouse integrin beta 6 (Itgb6), transcript variant 1

Product Images

Circular map for MR210654

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Itgb6
• Synonyms: 2210409C20Rik; 4831415H04Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210654 representing NM_001159564
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGATTGAGCTGGTCTGCCTGTTTCTGCTACTTCTAGGAAGGAATGATCACGTCCAAGGTGGCTGTG
  CCTGGGGTGGTGCAGAAAGCTGCTCAGACTGCCTGCTCACAGGACCCCACTGCGCCTGGTGCTCCCAGGA
  GAATTTCACCCACCTGTCTGGAGCTGGCGAGAGGTGCGACACCCCAGCGAATCTTTTAGCCAAAGGATGT
  CAACTACCCTTCATTGAAAACCCTGTCTCCCGCATAGAAGTCCTTCAAAATAAGCCTCTCAGCGTGGGCC
  GACAGAAGAACAGTTCTGACATTGTTCAGATTGCTCCTCAAAGCTTGGTTCTTAAATTGAGACCAGGGCG
  TGAGCAGACTCTGCAAGTGCAGGTCCGCCAAACTGAAGATTACCCAGTAGATCTGTATTACCTCATGGAC
  CTCTCCGCCTCTATGGATGACGACCTCAACACCATCAAAGAGCTTGGCTCCCGGCTGGCCAAAGAGATGT
  CCAAACTAACCAGCAACTTTAGACTGGGCTTTGGCTCTTTTGTGGAAAAGCCCGTTTCTCCTTTTATGAA
  AACAACCCCAGAGGAAATCACCAACCCTTGCAGTAGTATCCCCTATTTCTGCTTACCTACATTTGGATTC
  AAGCACATTTTGCCATTAACTGATGATGCCGAGAGATTCAATGAAATTGTGAGGAAACAGAAGATTTCTG
  CTAATATTGACACACCTGAAGGTGGATTTGATGCAATCATGCAGGCTGCTGTGTGTAAGGAAAAGATTGG
  CTGGCGCAATGACTCGCTCCACCTCCTGGTTTTTGTGAGTGATGCCGATTCTCATTTTGGAATGGACAGC
  AAGCTGGCAGGCATTGTCATTCCCAATGATGGACTCTGTCACTTGGACCACAGGAATGAATATTCCATGT
  CAACTGTCTTGGAATATCCAACTATCGGCCAACTCATTGATAAACTGGTACAAAACAACGTGCTACTGAT
  CTTTGCAGTCACCCAAGAACAAGTCCATCTGTATGAGAACTATGCAAAACTCATTCCTGGAGCAACCGTG
  GGACTGCTTCAGAAGGATTCTGGGAACATCCTTCAGCTGATCATCTCCGCTTATGAAGAACTGCGGTCTG
  AGGTGGAACTGGAAGTGTTAGGGGACACAGAAGGACTCAACCTGTCTTTCACAGCTCTCTGTAACAATGG
  TGTCCTCTTCCCACACCAAAAGAAATGCTCCCACATGAAAGTTGGAGACACAGCATCCTTCAATGTGACT
  GTGAGCGTATCCAACTGTGAGAAAAGAAGCAGGAACCTCATCATAAAGCCAGTGGGGCTGGGGGACACCC
  TAGAAATACTCGTCAGTGCAGAATGTGACTGCGACTGCCAGAGAGAAATAGAAACTAACAGCTCTAAGTG
  TCACAATGGGAATGGCTCCTTCCAGTGTGGGGTGTGTACCTGCAACCCCGGCCACATGGGTCCTCACTGC
  GAGTGTGGTGAGGATATGGTGAGCACGGATTCCTGCAAGGAGTCCCCGGGTCACCCTTCATGCAGTGGAA
  GGGGTGACTGCTATTGTGGGCAGTGCATCTGCCACTTATCGCCGTACGGAAGCATCTACGGACCTTACTG
  CCAGTGTGACAACTTCTCCTGTCTGAGACACAAAGGCCTTCTCTGTGGAGATAACGGTGACTGTGACTGT
  GGCGAGTGTGTGTGCCGGGATGGCTGGACAGGTGAATACTGTAACTGTACAACCAACAGGGACTCGTGTA
  CATCTGAGGATGGAGTGCTGTGCAGTGGACGTGGGGACTGTGTCTGTGGCAAGTGTGTCTGCAGAAACCC
  TGGAGCCTCAGGACCCACCTGTGAACGCTGTCCTACCTGTGGCGACCCCTGTAACTCTAAACGGAGTTGC
  ATCGAGTGCTACCTGTCTGCAGATGGCCAGGCCCAAGAAGAGTGTGCTGACAAGTGTAAAGCCATTGGTG
  CCACCATCAGTGAGGAAGATTTTTCAAAGGATACTTCTGTCTCCTGCTCTCTACAAGGAGAAAATGAATG
  CCTTATTACATTCCTAATAACTACGGATAATGAAGGGAAAACCATCATTCACAACATCAATGAAAAAGAC
  TGCCCCAAACCTCCAAACATTCCCATGATCATGTTGGGGGTGTCACTGGCGATCCTGCTCATCGGAGTTG
  TGCTACTGTGCATTTGGAAGCTGCTGGTATCATTTCATGACCGGAAGGAAGTTGCTAAATTTGAAGCAGA
  ACGCTCTAAGGCCAAGTGGCAAACGGGAACCAATCCTCTGTACCGAGGTTCCACCAGCACTTTTAAGAAC
  GTGACCTACAAGCACAGGGAGAAGCACAAAGCAGGCCTTTCCTCAGATGGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210654 representing NM_001159564
  Red=Cloning site Green=Tags(s)
  
  MGIELVCLFLLLLGRNDHVQGGCAWGGAESCSDCLLTGPHCAWCSQENFTHLSGAGERCDTPANLLAKGC
  QLPFIENPVSRIEVLQNKPLSVGRQKNSSDIVQIAPQSLVLKLRPGREQTLQVQVRQTEDYPVDLYYLMD
  LSASMDDDLNTIKELGSRLAKEMSKLTSNFRLGFGSFVEKPVSPFMKTTPEEITNPCSSIPYFCLPTFGF
  KHILPLTDDAERFNEIVRKQKISANIDTPEGGFDAIMQAAVCKEKIGWRNDSLHLLVFVSDADSHFGMDS
  KLAGIVIPNDGLCHLDHRNEYSMSTVLEYPTIGQLIDKLVQNNVLLIFAVTQEQVHLYENYAKLIPGATV
  GLLQKDSGNILQLIISAYEELRSEVELEVLGDTEGLNLSFTALCNNGVLFPHQKKCSHMKVGDTASFNVT
  VSVSNCEKRSRNLIIKPVGLGDTLEILVSAECDCDCQREIETNSSKCHNGNGSFQCGVCTCNPGHMGPHC
  ECGEDMVSTDSCKESPGHPSCSGRGDCYCGQCICHLSPYGSIYGPYCQCDNFSCLRHKGLLCGDNGDCDC
  GECVCRDGWTGEYCNCTTNRDSCTSEDGVLCSGRGDCVCGKCVCRNPGASGPTCERCPTCGDPCNSKRSC
  IECYLSADGQAQEECADKCKAIGATISEEDFSKDTSVSCSLQGENECLITFLITTDNEGKTIIHNINEKD
  CPKPPNIPMIMLGVSLAILLIGVVLLCIWKLLVSFHDRKEVAKFEAERSKAKWQTGTNPLYRGSTSTFKN
  VTYKHREKHKAGLSSDG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001159564
• ORF Size: 2361 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001159564.1, NP_001153036.1
• RefSeq Size: 4894 bp
• RefSeq ORF: 2364 bp
• Locus ID: 16420
• UniProt ID: Q9Z0T9
• Cytogenetics: 2 34.81 cM
• MW: 86.5 kDa
• Gene Summary: Integrin alpha-V:beta-6 (ITGAV:ITGB6) is a receptor for fibronectin and cytotactin (By similarity). It recognizes the sequence R-G-D in its ligands (PubMed:10025398). ITGAV:ITGB6 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (By similarity). Integrin alpha-V:beta-6 (ITGAV:ITGB6) mediates R-G-D-dependent release of transforming growth factor beta-1 (TGF-beta-1) from regulatory Latency-associated peptide (LAP), thereby playing a key role in TGF-beta-1 activation (PubMed:10025398).[UniProtKB/Swiss-Prot Function]