Lrrc41 (NM_153521) Mouse Tagged ORF Clone

CAT#: MR210646

Lrrc41 (Myc-DDK-tagged) - Mouse leucine rich repeat containing 41 (Lrrc41)

Product Images

Circular map for MR210646

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lrrc41
• Synonyms: AA409966; AW555107; D630045E04Rik; D730026A16Rik; MUF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210646 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCCACGTCCCGGGAGGCGGCGCCAGCGAAGAGCTCGGCCTCGGGCCCCAGCGCTCCCCCCGCCC
  TGTTCGAGCTGTGCGGGCGGGCAGTGAGCGCCCATATGGGGGTTCTGGAGAGCGGGGTGTGGGCCCTCCC
  AGGCCCAATACTTCAAAGCATCTTACCTCTGCTCAATATATATTACTTGGAGAGGATTGAGGAAACTGCT
  CTCAAAAAAGGTCTCTCTACTCAAGCCATCTGGCGCCGACTCTGGGATGAACTGATGAAGACAAGGCCTT
  CCAGTTTGGAGAGTGTGACTTGCTGGCGAGCCAAGTTTATGGAGGCCTTTTTTTCCCATGTTCTACGTGG
  AACCATTGATGTGTCTTCTGATAAACGTCTTTGTGATCAGCGCTTCTCACCTCTCCTGCACAGCTCCCGT
  CATGTCCGACAGCTAACCATCTGCAACATGCTGCAGGGAGCAACTGAGCTAGTGGCAGAGCCCAACCGTA
  GGGTTCTAGAGACCCTGGCCAGTTCTCTGCATACCCTCAAGTTCCGCCACCTGCTGTTCTCTGATGTGGC
  TGCTCAGCAGTCACTTCGACAGCTGCTGCATCAGCTCATCCACCATGGGGCTGTTAGCCAAGTGTCGCTG
  TACTCCTGGCCTGTGCCCGAGTCAGCCCTTTTCATCCTTATTCTTACCATGAGTGCTGGCTTTTGGCAAC
  CAGGCCCAGGTAGCTTGCCTTGCCGCCTCTGTGGTGAAGCCTCCCGAGGCCGGGCCCCATCTAGAGATGA
  AGGGTCCTTATTGCTAGGCTCTCGCAGGCCTCGGCGTGATGCAGCAGAGCGATGTGCTGCTGCACTAATG
  GCCACCCGGCGGAAGAGTGAAGTCAAGCAGATGCCCAGGGCTGTACCTCCCACTCGTGTAACACGCCGCA
  GCACACAAGAGAGCCTGGCAATAGGTGGAACAGACTCTAAGTTGTACCTCCCAGCCACCTCTTATGAGGC
  TTCTGGCACAAAACAGCCATCTGCTCCAGCTGCAGCCTCTGCCTCCTCTTCCACATCATCCAAACGGGCT
  CCAGCTAGCTCAGCCTCACAGCCTAAGCCCTTAAAGCGTTTCAAACGAGCTGCAGGGAAGAAAGGGCCCC
  GCACCCGTCAGGGGTCTGGTGCAGAGTCTGAAGACCTGTATGACTTTGTTTTTATTGTGGCTGGTGAGAA
  GGAGGATGGTGAAGAGATGGAGATTGGGGAAGTAGCTTGTGGAGCTCTGGATGGGTCAGATCCCAGCTGT
  TTGGGGCTCCCAGCACTAGAGGCATCCCAGCGATTCCGCAGCATTTCCACCTTGGAGCTATTCACAGTTC
  CTCTCTCCACGGAGGCAGCTCTGACACTGTGCCATCTTCTGAGTTCCTGGGTATCACTGGAGAGCCTTAC
  ACTTTCCTACAATGGTCTGGGCTCTAATATCTTCCGCCTCCTGGACAGCCTACGGGCCCTGTCAGGCCAG
  GCTGGATGTCGCCTCCGTGCCCTGCATCTCAGTGACCTTTTCTCACCACTGCCCATCCTGGAATTGACCC
  GTGCCATTGTGCGAGCCTTGCCCCTGTTGCGGGTCCTGTCTATTCGTGTTGACCACCCAAGCCAGAGGGA
  CAACCCTGCTGTACCAGAGAATGCTGGGCCCCCTGGACACATAGTTGGGGATGAAGAAATACCAGAAAAC
  TGCCTCGAGCAGTTGGAAATGGGATTTCCACGAGGAGCCCAGCCAGCCCCACTGCTCTGCTCTGTCCTCA
  AGGCCTCAGGTTCTCTACAGCAGCTGTCACTGGACAGTGCCACCTTTGCCTCGCCTCAAGATTTTGGGCT
  TGTGTTGCAAACACTCAAAGAACACAACCTATCCCTGAAGAGGCTGAGCTTCCATGATATGAATCTTGCA
  GACTGTCAGAGTGAAGTGCTTTTTTTGCTAAAGAATCTGACCCTTCAAGAGATTACCTTCTCCTTCTGCC
  GTCTGTTTGAGAAGCGCCCAGTCCAATTTCTTCCTGAGATGGTTGCTGCTATGAAGGGCAACTCTACACT
  GAAGGGCCTTCGACTACCAGGGAACCGCCTGGGGAACGCTGGCCTATTAGCCCTAGCAGATGTTTTCTCA
  GAGGATTCTTCCTCTTCTCTCTGTCAGCTGGACATCAGTTCCAACTGCATCAAGCCAGATGGGCTTTTGG
  AGTTTGCCAAACGACTGGAGCGCTGGGGCCGAGGAGCCTTTGGTCACCTACGCCTCTTCCAGAACTGGCT
  GGACCAGGATGCAGTCACAGCAAGAGAAGCAATCCGGCGACTCCGGGCTACCTGCCACGTGGTTAGCGAC
  TCATGGGACTCAACCCAGGCCTTTGCAGATTATGTCAGCACCATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210646 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEATSREAAPAKSSASGPSAPPALFELCGRAVSAHMGVLESGVWALPGPILQSILPLLNIYYLERIEETA
  LKKGLSTQAIWRRLWDELMKTRPSSLESVTCWRAKFMEAFFSHVLRGTIDVSSDKRLCDQRFSPLLHSSR
  HVRQLTICNMLQGATELVAEPNRRVLETLASSLHTLKFRHLLFSDVAAQQSLRQLLHQLIHHGAVSQVSL
  YSWPVPESALFILILTMSAGFWQPGPGSLPCRLCGEASRGRAPSRDEGSLLLGSRRPRRDAAERCAAALM
  ATRRKSEVKQMPRAVPPTRVTRRSTQESLAIGGTDSKLYLPATSYEASGTKQPSAPAAASASSSTSSKRA
  PASSASQPKPLKRFKRAAGKKGPRTRQGSGAESEDLYDFVFIVAGEKEDGEEMEIGEVACGALDGSDPSC
  LGLPALEASQRFRSISTLELFTVPLSTEAALTLCHLLSSWVSLESLTLSYNGLGSNIFRLLDSLRALSGQ
  AGCRLRALHLSDLFSPLPILELTRAIVRALPLLRVLSIRVDHPSQRDNPAVPENAGPPGHIVGDEEIPEN
  CLEQLEMGFPRGAQPAPLLCSVLKASGSLQQLSLDSATFASPQDFGLVLQTLKEHNLSLKRLSFHDMNLA
  DCQSEVLFLLKNLTLQEITFSFCRLFEKRPVQFLPEMVAAMKGNSTLKGLRLPGNRLGNAGLLALADVFS
  EDSSSSLCQLDISSNCIKPDGLLEFAKRLERWGRGAFGHLRLFQNWLDQDAVTAREAIRRLRATCHVVSD
  SWDSTQAFADYVSTM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153521
• ORF Size: 2358 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153521.1, NP_705741.2
• RefSeq Size: 2883 bp
• RefSeq ORF: 2424 bp
• Locus ID: 230654
• UniProt ID: Q8K1C9
• Cytogenetics: 4 D1
• MW: 85.8 kDa
• Gene Summary: Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.[UniProtKB/Swiss-Prot Function]