Pla2g4a (NM_008869) Mouse Tagged ORF Clone

CAT#: MR210449

Pla2g4a (Myc-DDK-tagged) - Mouse phospholipase A2, group IVA (cytosolic, calcium-dependent) (Pla2g4a)

Product Images

Circular map for MR210449

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pla2g4a
• Synonyms: cP; cPL; cPLA2; cPLA2-alpha; cPLA2alpha; Pla; Pla2g4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210449 representing NM_008869
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTTCATAGATCCTTATCAGCACATTATAGTGGAACACCAGTACTCCCATAAGTTTACTGTTGTGG
  TTCTACGTGCCACCAAAGTAACCAAGGGGACCTTTGGCGATATGCTGGACACTCCAGATCCTTATGTGGA
  ACTTTTCATCTCTACAACCCCTGACAGCAGGAAGCGAACGAGACACTTCAATAATGATATAAACCCCGTG
  TGGAATGAGACCTTTGAGTTCATTTTGGATCCTAATCAGGAAAATGTTTTGGAGATCACACTGATGGATG
  CCAACTACGTCATGGATGAAACCCTAGGCACAGCTACATTCCCTGTATCTTCAATGAAAGTGGGAGAGAA
  GAAAGAAGTCCCTTTTATTTTCAACCAAGTCACTGAAATGATTCTGGAAATGTCTCTGGAAGTTTGTTCA
  TGCCCAGACCTACGGTTCAGCATGGCACTGTGTGATCAGGAGAAAACTTTCAGACAGCAGAGGAAAGAGA
  ACATAAAAGAGAACATGAAGAAACTTTTGGGTCCAAAAAAGAGTGAGGGGCTTTATTCCACACGTGATGT
  GCCGGTGGTGGCCATTTTGGGTTCAGGTGGGGGTTTCCGGGCCATGGTGGGATTCTCTGGTGTGATGAAG
  GCACTGTATGAGTCGGGGATTTTGGACTGTGCTACATACATTGCTGGTCTTTCTGGATCCACATGGTACA
  TGTCAACCTTGTACTCTCACCCCGATTTTCCAGAGAAAGGTCCCGAGGAGATTAATGAAGAGCTAATGAA
  AAATGTCAGCCACAACCCTCTCTTACTTCTTACACCACAGAAAGTTAAAAGATACGTTGAGTCTTTATGG
  AAGAAGAAAAGTTCTGGCCAGCCTGTCACCTTTACTGACATCTTTGGGATGCTAATAGGAGAAACACTAA
  TTCAAAATAGGATGAGCATGACCCTGAGTAGTTTGAAGGAAAAGGTCAATGCCGCCCGGTGTCCTTTGCC
  TCTCTTCACGTGTCTCCACGTCAAACCTGATGTGTCAGAGCTGATGTTTGCCGATTGGGTGGAATTTAGT
  CCATATGAGATTGGCATGGCAAAATATGGTACCTTTATGGCTCCTGACCTATTTGGAAGCAAGTTTTTTA
  TGGGAACAGTTGTAAAAAAATATGAAGAAAACCCCTTGCATTTCTTGATGGGTGTCTGGGGCAGTGCCTT
  TTCTATACTGTTCAACAGAGTTTTGGGAGTTTCTGGCTCACAGAATAAAGGCTCTACAATGGAAGAGGAA
  TTAGAAAATATTACAGCAAAGCACATCGTGAGTAATGACAGCTCCGACAGTGATGATGAGGCTCAAGGAC
  CCAAAGGCACCGAGAATGAAGAAGCTGAAAAAGAGTACCAAAGCGACAACCAAGCAAGTTGGGTCCATCG
  GATGCTAATGGCCTTGGTGAGCGACTCGGCTTTATTCAATACCCGAGAAGGACGTGCCGGAAAGGTGCAT
  AACTTCATGCTGGGCTTGAATCTCAACACATCATATCCACTGTCTCCCCTGAGAGACTTCAGCTCTCAGG
  ATTCCTTCGATGACGAGCTCGACGCAGCGGTAGCAGATCCAGATGAATTTGAACGAATATATGAACCACT
  GGATGTCAAAAGTAAGAAGATTCATGTGGTAGATAGTGGGCTCACATTTAACCTGCCATATCCCTTGATT
  CTTCGACCTCAGAGAGGTGTGGATCTTATCATCTCCTTTGACTTTTCTGCAAGGCCGAGTGACACCAGTC
  CCCCTTTCAAGGAACTTCTGCTTGCAGAGAAGTGGGCGAAAATGAACAAGCTTCCCTTTCCAAAGATCGA
  TCCTTATGTGTTTGATCGGGAAGGATTAAAGGAATGCTATGTTTTTAAACCTAAGAATCCTGATGTGGAG
  AAGGATTGCCCAACCATTATCCACTTTGTTCTGGCCAACATCAACTTCAGAAAGTACAAGGCCCCAGGTG
  TTCTAAGGGAAACCAAAGAAGAGAAAGAAATTGCTGACTTTGACATTTTTGATGACCCCGAATCGCCATT
  TTCAACCTTCAACTTTCAGTATCCCAATCAAGCATTCAAAAGGCTTCACGATTTGATGTACTTCAACACA
  CTGAACAACATTGATGTGATAAAGGATGCCATTGTTGAGAGCATTGAATACAGAAGACAGAACCCATCTC
  GTTGCTCTGTTTCCCTCAGTAATGTTGAAGCAAGAAAATTCTTCAATAAGGAGTTTCTAAGTAAACCCAC
  TGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210449 representing NM_008869
  Red=Cloning site Green=Tags(s)
  
  MSFIDPYQHIIVEHQYSHKFTVVVLRATKVTKGTFGDMLDTPDPYVELFISTTPDSRKRTRHFNNDINPV
  WNETFEFILDPNQENVLEITLMDANYVMDETLGTATFPVSSMKVGEKKEVPFIFNQVTEMILEMSLEVCS
  CPDLRFSMALCDQEKTFRQQRKENIKENMKKLLGPKKSEGLYSTRDVPVVAILGSGGGFRAMVGFSGVMK
  ALYESGILDCATYIAGLSGSTWYMSTLYSHPDFPEKGPEEINEELMKNVSHNPLLLLTPQKVKRYVESLW
  KKKSSGQPVTFTDIFGMLIGETLIQNRMSMTLSSLKEKVNAARCPLPLFTCLHVKPDVSELMFADWVEFS
  PYEIGMAKYGTFMAPDLFGSKFFMGTVVKKYEENPLHFLMGVWGSAFSILFNRVLGVSGSQNKGSTMEEE
  LENITAKHIVSNDSSDSDDEAQGPKGTENEEAEKEYQSDNQASWVHRMLMALVSDSALFNTREGRAGKVH
  NFMLGLNLNTSYPLSPLRDFSSQDSFDDELDAAVADPDEFERIYEPLDVKSKKIHVVDSGLTFNLPYPLI
  LRPQRGVDLIISFDFSARPSDTSPPFKELLLAEKWAKMNKLPFPKIDPYVFDREGLKECYVFKPKNPDVE
  KDCPTIIHFVLANINFRKYKAPGVLRETKEEKEIADFDIFDDPESPFSTFNFQYPNQAFKRLHDLMYFNT
  LNNIDVIKDAIVESIEYRRQNPSRCSVSLSNVEARKFFNKEFLSKPTV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008869
• ORF Size: 2244 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008869.4
• RefSeq Size: 2846 bp
• RefSeq ORF: 2247 bp
• Locus ID: 18783
• UniProt ID: P47713
• Cytogenetics: 1 63.51 cM
• MW: 85.7 kDa
• Gene Summary: The protein encoded by this gene is a member of the phospholipase A2 group IV family. This enzyme hydrolyzes membrane phospholipids, thereby releasing the polyunsaturated fatty acid, arachidonic acid. Arachidonic acid is further metabolized into eicosanoids such as leukotrienes, thromboxanes and prostaglandins, that play important roles in regulating diverse biological processes such as inflammatory responses, membrane and actin dynamics, and tumorigenesis. A rise in intracellular calcium levels results in binding of calcium to the C2 domain of this protein, and triggers the translocation from the cytosol to intracellular membranes, including the Golgi apparatus. Disruption of this gene in mice led to decreased levels of eicosonaoids and platelet-activating factor, decreased allergic symptoms, and impaired reproductive ability in females. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015]