Sv2a (NM_022030) Mouse Tagged ORF Clone

CAT#: MR210408

Sv2a (Myc-DDK-tagged) - Mouse synaptic vesicle glycoprotein 2 a (Sv2a)

Product Images

Circular map for MR210408

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sv2a
• Synonyms: AI746429; mKIAA0736
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210408 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAAGGCTTTCGAGACCGAGCAGCGTTCATCCGTGGGGCCAAAGACATTGCCAAGGAAGTGAAGA
  AGCATGCGGCCAAGAAGGTGGTGAAGGGCCTTGACAGAGTCCAGGATGAGTATTCCCGAAGGTCCTACTC
  CCGCTTTGAGGAGGAGGACGACGACGATGACTTCCCTGCCCCTGCGGACGGCTATTACCGCGGAGAAGGG
  GCCCAGGATGAGGAGGAAGGTGGCGCTTCTAGTGATGCCACCGAGGGCCACGATGAAGATGATGAGATCT
  ATGAGGGAGAATATCAGGGCATCCCCCGGGCAGAGTCTGGGGGCAAAGGCGAACGCATGGCAGATGGGGC
  ACCCCTGGCTGGAGTGAGAGGGGGCTTGAGTGATGGGGAGGGTCCCCCTGGGGGTCGGGGGGAGGCGCAG
  CGGCGTAAAGATCGGGAAGAATTGGCTCAGCAGTATGAAACCATCCTCCGGGAGTGTGGCCATGGCCGCT
  TCCAGTGGACACTCTACTTCGTGCTGGGTCTGGCGCTGATGGCAGATGGTGTAGAGGTCTTTGTGGTGGG
  CTTTGTGCTGCCCAGTGCTGAGAAAGACATGTGCCTGTCGGACTCCAACAAAGGCATGCTAGGCCTCATT
  GTGTACCTGGGCATGATGGTGGGGGCCTTCCTCTGGGGAGGCCTGGCTGATCGGCTGGGTCGGAGACAGT
  GTCTGCTCATCTCACTCTCAGTCAACAGCGTCTTCGCCTTCTTCTCGTCCTTCGTCCAGGGTTATGGAAC
  CTTTCTCTTCTGCCGCCTCCTTTCCGGGGTCGGGATTGGCGGTTCCATCCCCATAGTCTTCTCCTATTTT
  TCGGAGTTTCTGGCGCAGGAGAAACGTGGGGAGCATTTGAGCTGGCTCTGTATGTTCTGGATGATCGGTG
  GAGTGTATGCAGCTGCAATGGCCTGGGCCATCATCCCTCACTATGGGTGGAGTTTCCAGATGGGCTCTGC
  TTACCAGTTCCACAGCTGGAGGGTGTTTGTCCTCGTGTGTGCCTTTCCCTCTGTGTTTGCCATCGGGGCT
  CTGACTACGCAGCCAGAGAGTCCCCGCTTCTTCCTAGAGAATGGGAAGCATGACGAAGCCTGGATGGTAC
  TGAAGCAGGTTCACGACACCAACATGCGAGCCAAGGGCCACCCTGAGCGCGTCTTCTCAGTGACCCACAT
  TAAAACGATTCATCAAGAGGATGAATTGATTGAGATCCAGTCTGACACAGGAACCTGGTACCAGCGCTGG
  GGAGTACGGGCTTTGAGCCTTGGGGGTCAGGTTTGGGGGAATTTCCTCTCCTGCTTCAGTCCAGAGTATC
  GGCGCATCACGCTGATGATGATGGGTGTGTGGTTCACCATGTCTTTCAGCTACTATGGTTTGACTGTCTG
  GTTTCCCGACATGATCCGCCATCTCCAGGCTGTGGACTATGCAGCCCGAACCAAAGTGTTCCCAGGGGAG
  CGCGTGGAGCATGTGACGTTTAACTTCACACTGGAGAATCAGATCCACCGAGGGGGACAGTACTTCAATG
  ACAAGTTCATCGGGCTGCGTCTGAAGTCAGTGTCCTTTGAGGATTCCCTGTTTGAGGAGTGTTACTTTGA
  AGATGTTACATCCAGCAACACATTCTTCCGCAACTGCACGTTCATCAACACTGTGTTCTATAACACTGAC
  CTATTTGAGTACAAGTTCGTGAACAGCCGGCTGGTGAACAGCACGTTCCTGCACAATAAGGAAGGCTGCC
  CGCTAGACGTGACGGGGACAGGCGAAGGTGCCTACATGGTGTACTTTGTCAGCTTCTTGGGGACACTGGC
  TGTGCTTCCTGGAAACATTGTGTCTGCTCTGCTCATGGACAAGATTGGCAGGCTCAGAATGCTTGCTGGT
  TCCAGTGTGTTGTCCTGTGTGTCCTGCTTCTTCCTGTCTTTTGGGAACAGCGAGTCAGCCATGATCGCTC
  TGCTCTGCCTTTTTGGGGGAGTTAGCATCGCATCCTGGAACGCGCTGGACGTGCTGACCGTTGAGCTCTA
  CCCTTCCGACAAGAGGACTACTGCCTTTGGCTTCCTGAATGCCCTGTGTAAGCTGGCAGCTGTGCTGGGG
  ATCAGCATCTTCACATCCTTTGTGGGCATCACCAAGGCTGCTCCCATTCTCTTTGCCTCGGCTGCTCTTG
  CCCTTGGTAGCTCTCTGGCTCTGAAGCTGCCTGAGACCCGGGGACAGGTGCTGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210408 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEEGFRDRAAFIRGAKDIAKEVKKHAAKKVVKGLDRVQDEYSRRSYSRFEEEDDDDDFPAPADGYYRGEG
  AQDEEEGGASSDATEGHDEDDEIYEGEYQGIPRAESGGKGERMADGAPLAGVRGGLSDGEGPPGGRGEAQ
  RRKDREELAQQYETILRECGHGRFQWTLYFVLGLALMADGVEVFVVGFVLPSAEKDMCLSDSNKGMLGLI
  VYLGMMVGAFLWGGLADRLGRRQCLLISLSVNSVFAFFSSFVQGYGTFLFCRLLSGVGIGGSIPIVFSYF
  SEFLAQEKRGEHLSWLCMFWMIGGVYAAAMAWAIIPHYGWSFQMGSAYQFHSWRVFVLVCAFPSVFAIGA
  LTTQPESPRFFLENGKHDEAWMVLKQVHDTNMRAKGHPERVFSVTHIKTIHQEDELIEIQSDTGTWYQRW
  GVRALSLGGQVWGNFLSCFSPEYRRITLMMMGVWFTMSFSYYGLTVWFPDMIRHLQAVDYAARTKVFPGE
  RVEHVTFNFTLENQIHRGGQYFNDKFIGLRLKSVSFEDSLFEECYFEDVTSSNTFFRNCTFINTVFYNTD
  LFEYKFVNSRLVNSTFLHNKEGCPLDVTGTGEGAYMVYFVSFLGTLAVLPGNIVSALLMDKIGRLRMLAG
  SSVLSCVSCFFLSFGNSESAMIALLCLFGGVSIASWNALDVLTVELYPSDKRTTAFGFLNALCKLAAVLG
  ISIFTSFVGITKAAPILFASAALALGSSLALKLPETRGQVLQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022030
• ORF Size: 2229 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022030.3, NP_071313.1
• RefSeq Size: 3920 bp
• RefSeq ORF: 2229 bp
• Locus ID: 64051
• UniProt ID: Q9JIS5
• Cytogenetics: 3 F2.1
• MW: 82.6 kDa
• Gene Summary: Plays a role in the control of regulated secretion in neural and endocrine cells, enhancing selectively low-frequency neurotransmission. Positively regulates vesicle fusion by maintaining the readily releasable pool of secretory vesicles.[UniProtKB/Swiss-Prot Function]