Abcd2 (NM_011994) Mouse Tagged ORF Clone

CAT#: MR210402

Abcd2 (Myc-DDK-tagged) - Mouse ATP-binding cassette, sub-family D (ALD), member 2 (Abcd2)

Product Images

Circular map for MR210402

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Abcd2
• Synonyms: A; ABC3; ABC39; AL; ALDL1; ALDR; ALDRP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210402 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATACACATGCTAAATGCAGCAGCCTATCGGGTGAAATGGACCAGATCCGGTGCTGCTAAAAGGGCTG
  CCTGCCTGGTGGCTGCGGCATATGCTCTGAAAACCCTCTATCCCATCATTGGCAAGCGTTTAAAGCAGCC
  TGGCCACAGGAAGGCAAAAGCAGAAGCTTACTCGCCTGCAGAGAACAGAGAAATACTGCATTGCACGGAG
  ATCATCTGTAAAAAACCTGCGCCGGGACTAAATGCAGCTTTTTTCAAACAGCTACTAGAACTTCGGAAAA
  TCCTCTTTCCAAAACTTGTGACCACTGAAACGGGGTGGCTCTGCCTCCACTCGGTGGCTCTAATCTCAAG
  AACATTTCTCTCTATTTATGTGGCTGGTCTGGATGGGAAAATCGTGAAAAGCATCGTGGAAAAGAAGCCT
  CGGACTTTCATCATCAAATTAATCAAGTGGCTTATGATTGCTATCCCTGCTACCTTTGTCAACAGTGCTA
  TCAGGTACCTGGAATGCAAACTGGCATTGGCCTTTAGAACTCGCTTAGTAGACCATGCCTATGAGACCTA
  TTTCGCAAATCAGACTTATTATAAGGTGATAAATATGGATGGGAGGCTGGCAAACCCTGACCAGTCTCTT
  ACCGAAGACATTATGATGTTCTCGCAATCTGTGGCTCACCTGTATTCCAACCTTACCAAACCTATTTTAG
  ATGTCATTCTAACCTCCTATACTCTCATCCGGACAGCTACATCCAGAGGAGCAAGCCCTATAGGGCCCAC
  CCTGTTAGCAGGACTTGTCGTGTATGCCACTGCTAAAGTACTGAAAGCTTGCTCGCCCAAATTTGGTTCG
  CTGGTGGCTGAAGAAGCCCACAGGAAAGGCTACCTGCGGTATGTCCACTCCCGAATCATAGCCAATGTAG
  AAGAAATTGCCTTCTACAGAGGACATAAGGTAGAAATGAAGCAGCTGCAGAAATGTTACAAGGCTTTAGC
  TTACCAGATGAACCTGATTTTATCCAAACGTTTATGGTACATCATGATAGAACAATTCTTGATGAAGTAT
  GTGTGGAGCAGCTGTGGACTAATTATGGTGGCTATACCCATTATCACTGCAACGGGCTTTGCAGATGGTG
  ATCTGGAGGATGGTCCAAAGCAGGCTATGGTTAGCGATCGGACAGAGGCCTTCACCACTGCCCGGAACTT
  ACTGGCCTCTGGAGCTGATGCAATTGAAAGGATTATGTCTTCATACAAAGAGATCACTGAACTAGCAGGT
  TATACTGCTAGAGTATACAATATGTTCTGGGTCTTCGATGAAGTGAAGAGAGGCATTTATAAGAGAACTG
  TCACTCAGGAACCTGAAAACCATAGCAAGCGTGGAGGTAACCTGGAACTACCCCTCAGCGACACCCTGGC
  CATCAAAGGAACAGTTATTGATGTGGATCATGGAATCATTTGTGAAAATGTTCCCATAATTACACCAGCG
  GGCGAAGTGGTGGCTTCCAGGCTAAACTTCAAAGTGGAAGAAGGGATGCATCTCTTGATAACTGGTCCCA
  ACGGTTGTGGGAAAAGCTCTCTCTTCAGAATCTTAAGCGGGCTGTGGCCTGTGTATGAAGGAGTCCTTTA
  TAAACCGCCTCCCCAACATATGTTCTATATTCCACAGAGGCCATACATGTCTCTTGGAAGTCTCCGGGAT
  CAAGTCATTTACCCTGACTCAGCGGATGACATGCGTGAGAAAGGTTACACTGACCAAGACCTAGAACGCA
  TCCTGCACAGCGTGCACCTCTACCACATAGTTCAAAGAGAAGGAGGATGGGATGCAGTCATGGACTGGAA
  AGATGTCCTTTCCGGAGGGGAGAAGCAGAGAATGGGCATGGCGCGGATGTTTTACCATAAACCGAAGTAT
  GCATTGCTGGATGAATGTACCAGTGCCGTGAGCATCGACGTTGAAGGAAAGATATTTCAGGCTGCTATTG
  GGGCTGGGATTTCCCTACTCTCCATAACACACAGGCCTTCTCTGTGGAAATACCACACTCATCTATTACA
  ATTCGATGGCGAAGGAGGCTGGCGCTTTGAACAGTTGGACACTGCTATCCGTTTAACGTTGAGTGAGGAA
  AAGCAAAAGTTGGAGTCGCAGCTCGCTGGAATTCCCAAAATGCAACAGAGACTCAACGAACTATGCAAAA
  TTCTGGGGGAAGACTCGGTGCTGAAAACAATCCAAACTCCAGAAAAGACATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210402 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MIHMLNAAAYRVKWTRSGAAKRAACLVAAAYALKTLYPIIGKRLKQPGHRKAKAEAYSPAENREILHCTE
  IICKKPAPGLNAAFFKQLLELRKILFPKLVTTETGWLCLHSVALISRTFLSIYVAGLDGKIVKSIVEKKP
  RTFIIKLIKWLMIAIPATFVNSAIRYLECKLALAFRTRLVDHAYETYFANQTYYKVINMDGRLANPDQSL
  TEDIMMFSQSVAHLYSNLTKPILDVILTSYTLIRTATSRGASPIGPTLLAGLVVYATAKVLKACSPKFGS
  LVAEEAHRKGYLRYVHSRIIANVEEIAFYRGHKVEMKQLQKCYKALAYQMNLILSKRLWYIMIEQFLMKY
  VWSSCGLIMVAIPIITATGFADGDLEDGPKQAMVSDRTEAFTTARNLLASGADAIERIMSSYKEITELAG
  YTARVYNMFWVFDEVKRGIYKRTVTQEPENHSKRGGNLELPLSDTLAIKGTVIDVDHGIICENVPIITPA
  GEVVASRLNFKVEEGMHLLITGPNGCGKSSLFRILSGLWPVYEGVLYKPPPQHMFYIPQRPYMSLGSLRD
  QVIYPDSADDMREKGYTDQDLERILHSVHLYHIVQREGGWDAVMDWKDVLSGGEKQRMGMARMFYHKPKY
  ALLDECTSAVSIDVEGKIFQAAIGAGISLLSITHRPSLWKYHTHLLQFDGEGGWRFEQLDTAIRLTLSEE
  KQKLESQLAGIPKMQQRLNELCKILGEDSVLKTIQTPEKTS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011994
• ORF Size: 2226 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011994.1
• RefSeq Size: 5540 bp
• RefSeq ORF: 2226 bp
• Locus ID: 26874
• UniProt ID: Q61285
• Cytogenetics: 15 E3
• MW: 83.5 kDa
• Gene Summary: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ALD subfamily, which is involved in peroxisomal import of fatty acids and/or fatty acyl-CoAs in the organelle. All known peroxisomal ABC transporters are half transporters which require a partner half transporter molecule to form a functional homodimeric or heterodimeric transporter. The function of this peroxisomal membrane protein is unknown; however this protein is speculated to function as a dimerization partner of Abcd1 and/or other peroxisomal ABC transporters. Mutations in the human gene have been observed in patients with adrenoleukodystrophy, a severe demyelinating disease. This gene has been identified as a candidate for a modifier gene, accounting for the extreme variation among adrenoleukodystrophy phenotypes. This gene is also a candidate for a complement group of Zellweger syndrome, a genetically heterogeneous disorder of peroxisomal biogenesis. [provided by RefSeq, Jul 2008]