Maml2 (BC053388) Mouse Tagged ORF Clone

CAT#: MR210386

Maml2 (Myc-DDK-tagged) - Mouse mastermind like 2 (Drosophila) (cDNA clone MGC:60867 IMAGE:30054622)

Product Images

Circular map for MR210386

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Maml2
• Synonyms: 5930431H10; BC032967
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210386 representing BC053388
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGACACAGCGCCCCCGCAGGCCCCCGCAGGAGGGTTGGGAGGGGCTTCTGGGGCGGGGCTCCTTG
  GAGGGGGCTCAGTCACTCCGAGAGTGCACAGTGCTATTGTGGAACGCCTGCGGGCTCGCATTGCTGTCTG
  CCGACAGCACCACCTGAGCTGTGAAGGACGCTATGAACGTGGTAGGGCTGAGAGCTCAGACCGAGAGAGG
  GAGAGCACCGTGCAGCTTCTCAGCCTGGTGCAGCATGGTCAGGGGGCAAGGAAGGCTAGCAAACATGCTA
  AGGCTACAGTCGCGACTGCCACTACCACAGCTCCTCCACCGCCCCCTACTGCCCCTCCTGTGGCCTCCCA
  AGCGGCAGCCACGGCCCCACCACCTCCACCAGACTATCACCATCACCATCAGCAGCACCGGCTGACCAGT
  AATGATAACGGTGGGATAAACCGAGAGCAGCCGCCAGCTTCGACCCCGGGGGACCAGCGGAACTCAGCCC
  TGCTTGCGCTCCAGGGCTCTTTGAAAAGAAAGCAAATAGTCAACTTGTCTCCTGCCAACAGCAAGAGGCC
  CAATGGCTTTGTTGACAGCTCATTTCTTGACATCAAAAGGATCAGAGTTGGGGAGAACCTTTCTACAGGT
  CAGGGGGGCCTCCCAGTAAATAATGGACAAAGTCAAATGATGCCAGGGCCCTTGACCATGAATCAAGCAC
  CCCTGAGGAAGACTAATGCTCTACCACCTCCTGCACATTCCCCTGGCAACAATTTGTTTAACATGGGCTT
  AAAGGAGGTGAAGAAAGAACCTGGGGAGACCCTGTCTTGCAGTAAGCACATGAGTGACCAAATGACACAA
  GAGAGTGTTTTTACTAATAGGTTTGGAGATGACCCTGGAGATCAACTGATGGACCCTGAGCTACAGGAAC
  TGTTCAATGAACTAACCAACATATCAGTGCCTCCCATGAGTGACCTGGAGCTGGAGAACATGATCAATGC
  TACCATTAAGCAAGATGACCCGTTTGGCATTGACTTGGGTCAGCAGAGCCAGAGGAGCACACCTAGACCC
  TCCCTGCCCATGGAGAAGATCATTATCAAAAGTGAATATTCACCTGGTTTGACACAAGGCACTTCAGGCT
  CTCCCCAGCTGAGACCCCCATCAGCTGGCCCTGCATTTTCCTTGGCTAACTCTGCACTCTCCACTTCATC
  CCCAATTCCCACAATTCCCCAGAGTCAGCCTCAGCTTCAGACAGTATCAGGAGCAAACAGGGCCCTGTCA
  AGCTGGCAAGAAGTATCCCATGCTCAACAGCTCAAGCAGATAGCTGCAAACCGCCAGCAGCATGCACGGA
  TGCAGCAGCAGCAACAGCAGCAGCAGCAGCAGCCATCTCCCAGCTGGCCAGCCTTGCAGTCCTCTGCTGG
  GCCATCCCCAGGAGCATTTGTGCAGGAAAAAATCCCTAGCCCTTCCTTCAGCCAGCAACCATTCAGTCCA
  CAGGGATCCCCCATGCCTGGGGTGACTGGCAGCAACACCCAGTCTAAAGCAGCGGCCAATTACACGTACA
  AGGCCAGCCCCTCAGCCCAGGCTGGACACCTGGATGTGCTTCTGCAGCAAAAGCCTCAGGAGCTCAATCG
  AAGCTTCATTAATAACCCACACCCAGCCTTGGAGCCCCGGCATGGCAATACCAAGCCTTTGTTCCATTTT
  AACTCAGACCAAGCAAACCAGCAGATGCCTTCTCTTTTGCCTTCCCAGAGCAAACCTTCTCTCTTGCACT
  ATACCCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAACAACAACAACAACAACAGCAGCAGCA
  ACAGCAGCAGCAACAACAACAACAGCAGCAGCAACAACAACAGCAGCAGCAGCAACAACAACAACAGCAA
  GGCTCCCTGGCAGCTCAGCAGCAGCAGCAGCAGCCACAGCCACAGAGTTCATTGGTAGCTCAGCAGCAGC
  AACAGCAGCAGCAGCAGCAGCAGCAAGGTTCATTGACAGCTCAGCAGCAGCAGCAACAGCAGCAGCAGCA
  GCAACCATCGCAACCCACCCATGCTTTATCAAGCCAGCCTTTGCTAAGGTCACCCTTACAACTTCAGCAA
  AAGATCATGCTTCAGAAAATGCAAACTCAGTCCATCACAGGCCTGGGATATCAAGTCACTCAACAGCACA
  GACAGGTAAGGGCTCTGGTACTTGGCCCCAGAACTCCCACCAGTGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210386 representing BC053388
  Red=Cloning site Green=Tags(s)
  
  MGDTAPPQAPAGGLGGASGAGLLGGGSVTPRVHSAIVERLRARIAVCRQHHLSCEGRYERGRAESSDRER
  ESTVQLLSLVQHGQGARKASKHAKATVATATTTAPPPPPTAPPVASQAAATAPPPPPDYHHHHQQHRLTS
  NDNGGINREQPPASTPGDQRNSALLALQGSLKRKQIVNLSPANSKRPNGFVDSSFLDIKRIRVGENLSTG
  QGGLPVNNGQSQMMPGPLTMNQAPLRKTNALPPPAHSPGNNLFNMGLKEVKKEPGETLSCSKHMSDQMTQ
  ESVFTNRFGDDPGDQLMDPELQELFNELTNISVPPMSDLELENMINATIKQDDPFGIDLGQQSQRSTPRP
  SLPMEKIIIKSEYSPGLTQGTSGSPQLRPPSAGPAFSLANSALSTSSPIPTIPQSQPQLQTVSGANRALS
  SWQEVSHAQQLKQIAANRQQHARMQQQQQQQQQQPSPSWPALQSSAGPSPGAFVQEKIPSPSFSQQPFSP
  QGSPMPGVTGSNTQSKAAANYTYKASPSAQAGHLDVLLQQKPQELNRSFINNPHPALEPRHGNTKPLFHF
  NSDQANQQMPSLLPSQSKPSLLHYTQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQ
  GSLAAQQQQQQPQPQSSLVAQQQQQQQQQQQQGSLTAQQQQQQQQQQQPSQPTHALSSQPLLRSPLQLQQ
  KIMLQKMQTQSITGLGYQVTQQHRQVRALVLGPRTPTSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC053388
• ORF Size: 2217 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC053388.1
• RefSeq Size: 2955 bp
• RefSeq ORF: 2219 bp
• Locus ID: 270118
• Cytogenetics: 9 A1
• MW: 108.4 kDa