Ctcf (NM_181322) Mouse Tagged ORF Clone

CAT#: MR210366

Ctcf (Myc-DDK-tagged) - Mouse CCCTC-binding factor (Ctcf)

Product Images

Circular map for MR210366

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ctcf
• Synonyms: AW108038
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210366 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGTGAGGCGGTTGAAGCCATTGTGGAGGAGTCTGAAACTTTCATTAAAGGAAAAGAAAGAAAGA
  CTTACCAGAGACGCCGGGAAGGGGGCCAGGAAGAAGATGCTTGCCACCTGCCCCAGAACCAGACAGATGG
  GGGTGAGGTGGTCCAGGATGTCAACAGCAGTGTACAGATGGTAATGATGGAACAGCTGGATCCTACCCTT
  CTCCAGATGAAGACTGAAGTCATGGAGGGTACAGTGGCTCCGGAAGCAGAGGCTGCAGTGGACGATACCC
  AGATCATAACCTTGCAGGTTGTAAATATGGAGGAACAGCCCATTAACATAGGAGAGCTTCAGCTTGTCCA
  AGTACCTGTTCCTGTGACGGTACCTGTTGCTACTACTTCAGTAGAAGAACTTCAGGGGGCTTATGAGAAT
  GAAGTGTCTAAAGAGGGCCTTGCAGAAAGTGAACCGATGATATGTCACACCTTACCTTTGCCTGAAGGAT
  TTCAGGTGGTGAAAGTGGGGGCCAATGGAGAAGTGGAGACACTAGAGCAGGGCGAGCTTCCTCCTCAGGA
  AGACTCTAGCTGGCAAAAAGACCCAGACTATCAGCCACCAGCCAAAAAAACAAAGAAAACCAAAAAGAGC
  AAACTTCGTTACACAGAAGAGGGCAAAGACGTGGATGTGTCTGTGTATGATTTTGAGGAAGAACAGCAGG
  AAGGACTGCTGTCTGAGGTTAATGCAGAGAAAGTAGTTGGTAATATGAAGCCTCCGAAGCCAACAAAAAT
  TAAAAAAAAAGGTGTAAAGAAAACATTCCAGTGTGAGCTTTGCAGTTACACATGTCCCCGGCGTTCAAAT
  TTGGATCGTCACATGAAAAGCCACACTGATGAGAGACCACACAAATGCCATCTGTGTGGCAGAGCATTCA
  GAACAGTGACCCTCCTGAGGAATCATCTGAACACACACACAGGTACTCGTCCTCACAAGTGCCCAGACTG
  CGATATGGCCTTTGTGACCAGTGGAGAATTGGTGCGGCATCGTCGTTATAAACACACTCATGAGAAACCA
  TTTAAGTGTTCCATGTGTGATTATGCCAGTGTAGAAGTCAGCAAATTAAAACGACACATTCGCTCTCATA
  CTGGAGAGCGCCCGTTCCAGTGCAGTTTGTGCAGTTATGCCAGCAGGGACACATACAAGCTGAAAAGGCA
  TATGAGAACCCATTCAGGGGAAAAACCTTATGAATGTTATATTTGTCACGCTCGGTTTACCCAGAGTGGT
  ACCATGAAGATGCACATTTTACAGAAGCACACAGAAAATGTGGCCAAATTTCATTGTCCCCATTGTGACA
  CTGTCATAGCCCGAAAAAGTGATTTGGGTGTCCACTTGCGAAAGCAGCATTCCTATATTGAACAGGGCAA
  AAAATGTCGCTACTGTGATGCTGTGTTTCATGAGCGATATGCTCTCATCCAGCATCAAAAATCACACAAA
  AATGAGAAGCGCTTCAAGTGTGACCAGTGTGATTATGCTTGTAGACAGGAGCGGCACATGATCATGCACA
  AGCGCACTCACACGGGGGAGAAGCCTTATGCCTGCAGCCACTGCGACAAGACCTTCCGCCAGAAACAGCT
  CCTCGACATGCATTTCAAGCGCTATCATGATCCCAACTTTGTCCCTGCTGCCTTTGTCTGTTCCAAGTGT
  GGGAAAACATTCACCCGCCGGAACACAATGGCAAGACATGCAGATAACTGTGCTGGTCCAGATGGCGTAG
  AGGGGGAAAATGGAGGGGAGACAAAGAAGAGCAAACGAGGAAGAAAAAGAAAGATGCGATCTAAAAAAGA
  AGACTCCTCTGACAGTGAAGAAAATGCTGAGCCGGATCTCGATGACAATGAGGAGGAGGAGGAGCCTGCT
  GTAGAAATTGAACCTGAGCCAGAGCCTCAGCCCCAGCCTCCGCCTCCACCTCAGCCTGTGGCCCCGGCCC
  CCCCACCTGCCAAGAAGAGAAGGGGGCGACCTCCTGGAAGAACCAACCAGCCCAAACAGAACCAGCCAAC
  AGCCATCATTCAGGTCGAAGATCAGAATACAGGTGCAATTGAGAACATTATAGTTGAAGTCAAAAAGGAG
  CCAGATGCCGAGCCTGCGGAGGGGGAAGAAGAGGAGGCTCAGGCAGCCACCACAGACGCCCCCAACGGAG
  ACCTCACGCCTGAGATGATCCTCAGCATGATGGACCGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210366 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEGEAVEAIVEESETFIKGKERKTYQRRREGGQEEDACHLPQNQTDGGEVVQDVNSSVQMVMMEQLDPTL
  LQMKTEVMEGTVAPEAEAAVDDTQIITLQVVNMEEQPINIGELQLVQVPVPVTVPVATTSVEELQGAYEN
  EVSKEGLAESEPMICHTLPLPEGFQVVKVGANGEVETLEQGELPPQEDSSWQKDPDYQPPAKKTKKTKKS
  KLRYTEEGKDVDVSVYDFEEEQQEGLLSEVNAEKVVGNMKPPKPTKIKKKGVKKTFQCELCSYTCPRRSN
  LDRHMKSHTDERPHKCHLCGRAFRTVTLLRNHLNTHTGTRPHKCPDCDMAFVTSGELVRHRRYKHTHEKP
  FKCSMCDYASVEVSKLKRHIRSHTGERPFQCSLCSYASRDTYKLKRHMRTHSGEKPYECYICHARFTQSG
  TMKMHILQKHTENVAKFHCPHCDTVIARKSDLGVHLRKQHSYIEQGKKCRYCDAVFHERYALIQHQKSHK
  NEKRFKCDQCDYACRQERHMIMHKRTHTGEKPYACSHCDKTFRQKQLLDMHFKRYHDPNFVPAAFVCSKC
  GKTFTRRNTMARHADNCAGPDGVEGENGGETKKSKRGRKRKMRSKKEDSSDSEENAEPDLDDNEEEEEPA
  VEIEPEPEPQPQPPPPPQPVAPAPPPAKKRRGRPPGRTNQPKQNQPTAIIQVEDQNTGAIENIIVEVKKE
  PDAEPAEGEEEEAQAATTDAPNGDLTPEMILSMMDR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181322
• ORF Size: 2211 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181322.3, NP_851839.1
• RefSeq Size: 3848 bp
• RefSeq ORF: 2211 bp
• Locus ID: 13018
• UniProt ID: Q61164
• Cytogenetics: 8 D3
• MW: 83.7 kDa
• Gene Summary: Chromatin binding factor that binds to DNA sequence specific sites. Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Acts as transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene. Also binds to the PLK and PIM1 promoters. Acts as a transcriptional activator of APP. Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression. Plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription. Seems to act as tumor suppressor. Plays a critical role in the epigenetic regulation. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2. Plays a critical role in gene silencing over considerable distances in the genome. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. Inversely, binding to target sites is prevented by CpG methylation. Plays an important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping (By similarity). Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory. Causes local loss of histone acetylation and gain of histone methylation in the beta-globin locus, without affecting transcription. When bound to chromatin, it provides an anchor point for nucleosomes positioning. Seems to be essential for homologous X-chromosome pairing. May participate with Tsix in establishing a regulatable epigenetic switch for X chromosome inactivation. May play a role in preventing the propagation of stable methylation at the escape genes from X-inactivation. Involved in sister chromatid cohesion. Associates with both centromeres and chromosomal arms during metaphase and required for cohesin localization to CTCF sites. Regulates asynchronous replication of IGF2/H19. Plays a role in the recruitment of CENPE to the pericentromeric/centromeric regions of the chromosome during mitosis (By similarity).[UniProtKB/Swiss-Prot Function]