Fbxo42 (NM_172518) Mouse Tagged ORF Clone

CAT#: MR210247

Fbxo42 (Myc-DDK-tagged) - Mouse F-box protein 42 (Fbxo42)

Product Images

Circular map for MR210247

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fbxo42
• Synonyms: 6720460I06Rik; mKIAA1332
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210247 representing NM_172518
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGCTCCTCGGACAGTGAAGATGACAGTGTCATGGCCGTGGACCAAGAAGAAACTGCACTGGAAG
  GGACAATGGAGCAAGATGAGGACCCCCACCCAGTGTTGGAGGTTGAGGAAACAAGACATAATAGGTCCAT
  GTCTGAGCTGCCGGAAGAGGTTTTGGAGTATATTCTGTCCTTCCTTTCACCATACCAGGAACACAAGACT
  GCAGCTCTGGTCTGCAAACAGTGGTATCGACTTATCAAAGGTGTGGCCCACCAGTGTTACCATGGCTTCA
  TGAAGGCTGTCCAGGAAGGAAACATTCAGTGGGAGAGCCGGACTTACCCTTATCCGGGAACCCCTATCAC
  TCAGCGCTTCTCCCACAGTGCGTGCTATTATGATGCCAATCAGTCTATGTATGTCTTTGGTGGCTGTACG
  CAGAGCAGTTGCAATGCTGCCTTCAATGACCTCTGGAGGCTAGACCTAAATAGCAAGGAGTGGATTCGAC
  CATTGGCCTCAGGGTCCTATCCTTCCCCCAAAGCTGGGGCCACTCTGGTCGTGTACAAGGACTTGCTAGT
  GCTATTTGGTGGCTGGACAAGGCCAAGTCCCTATCCCTTACACCAGCCAGAGAGATTCTTTGATGAAATT
  CACACGTACTCGCCGTCTAAAAACTGGTGGAACTGCATTGTCACCACGCATGGGCCTCCTCCCATGGCTG
  GCCATTCCTCCTGTGTGATAGGTGATAAAATGATTGTCTTCGGTGGCTCCTTAGGATCCCGGCAGATGAG
  CAATGAAGTCTGGGTCCTGGACCTGGAGCAGTGGGCGTGGTCCAAGCCGAACATCTCTGGCCCCAGTCCA
  CACCCTCGAGGTGGCCAGTCTCAGATTGTTATAGATGATACAACTCTTCTGATCCTTGGCGGGTGTGGCG
  GTCCCAATGCGCTGTTCAAAGATGCCTGGCTGCTGCACATGCACCCAGGTCCCTGGGCCTGGCAGCCACT
  CAAGGTAGAAAATGAAGACCATGGTGCCCCGGAGCTATGGTGTCATCCAGCCTGCCGGGTGGGACAGTGT
  GTGGTGGTCTTCAGCCAGGCGCCAAGCGGGAGAGCGCCCCTCAGCCCCAGTTTGAACTCTCGCCCGTCAC
  CTATCAGTGCCACTCCTCCAGCCCTGGTTCCTGAGACCCGAGAGTACCGCTCCCAGTCCCCGGTGAGGAG
  TATGGACGAAGCGCCCTGTGTTAATGGCCGCTGGGGAACACTGAGGCCCAGGGCTCAAAGGCAGACTCCC
  TCGGGTTCCCGGGAAGGGAGCCTCTCCCCAGCCAGAGGAGATGGCTCTCCCATTCTCAATGGTGGAAATT
  TGTCTCCAGGGACAGTAGCTGTCGGGGGTGCTTCCTTAGACAGCCCCGTACAGGTCGTGTCTCCAAGCAC
  TCCATCTGCCTCTGACGGCTACGACCTAAAAGTAGGACTTTCTCTAGCTCCTCGACGAGGGTCTTTACCA
  GATCAGAAGGACCTGAGGTTAAGTTCCATTGATCTGAATTGGGACCTGAAGTCTGCTTCCAGTAGTAGTC
  ACGTGGATAGTATAGACAACAGGACAGTTGCAGGGAGTGTGAGACACCCTCCAGAACAGACCAATGGTGT
  TCACACCCCACCACATGTGGCCAGTGCCCTTGCAGGGGCTGTATCTCCCGGTGCCCTGCGTCGGAGTTTG
  GAAGCCATCAAAGCGATGTCATCCAAAGGCCCCTCAGCCTCGGCAGCACTAAGTCCTCCTCTTGGGTCTT
  CTCCAAGCTCCCCTGGGAGTCAGAGCCTGAGCAGTGGAGAAACAGTGCCCAACTCCCGCCCAGGACCAGC
  CCAAGGAGATGGTCATTCCTTACCCCCGATTGCTCGGCGCCTGGGCCACCACCCTCCACAGTCCCTTAAT
  GTGGGTAAACCCTTGTACCAGAGTATGAACTGCAAACCTATGCAGATGTATGTGCTAGACATTAAAGACA
  CCAAGGAGAAGGGCCGAGTCAAGTGGAAAGTGTTCACAAGCAGCTCGGTGGTCGGGCCTCCTGAGACCAG
  CCTGCACACGGTGGTGCAGGGCCGAGGCGAGCTCATCGTATTTGGAGGGCTCATGGACAAGAAGCAGAAT
  GTGAAGTACTACCCAAAGACAAACGCCTTGTACTTTGTGCGCGCAAAGAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210247 representing NM_172518
  Red=Cloning site Green=Tags(s)
  
  MASSSDSEDDSVMAVDQEETALEGTMEQDEDPHPVLEVEETRHNRSMSELPEEVLEYILSFLSPYQEHKT
  AALVCKQWYRLIKGVAHQCYHGFMKAVQEGNIQWESRTYPYPGTPITQRFSHSACYYDANQSMYVFGGCT
  QSSCNAAFNDLWRLDLNSKEWIRPLASGSYPSPKAGATLVVYKDLLVLFGGWTRPSPYPLHQPERFFDEI
  HTYSPSKNWWNCIVTTHGPPPMAGHSSCVIGDKMIVFGGSLGSRQMSNEVWVLDLEQWAWSKPNISGPSP
  HPRGGQSQIVIDDTTLLILGGCGGPNALFKDAWLLHMHPGPWAWQPLKVENEDHGAPELWCHPACRVGQC
  VVVFSQAPSGRAPLSPSLNSRPSPISATPPALVPETREYRSQSPVRSMDEAPCVNGRWGTLRPRAQRQTP
  SGSREGSLSPARGDGSPILNGGNLSPGTVAVGGASLDSPVQVVSPSTPSASDGYDLKVGLSLAPRRGSLP
  DQKDLRLSSIDLNWDLKSASSSSHVDSIDNRTVAGSVRHPPEQTNGVHTPPHVASALAGAVSPGALRRSL
  EAIKAMSSKGPSASAALSPPLGSSPSSPGSQSLSSGETVPNSRPGPAQGDGHSLPPIARRLGHHPPQSLN
  VGKPLYQSMNCKPMQMYVLDIKDTKEKGRVKWKVFTSSSVVGPPETSLHTVVQGRGELIVFGGLMDKKQN
  VKYYPKTNALYFVRAKR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172518
• ORF Size: 2151 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172518.3, NP_766106.2
• RefSeq Size: 5943 bp
• RefSeq ORF: 2154 bp
• Locus ID: 213499
• UniProt ID: Q6PDJ6
• Cytogenetics: 4 D3
• MW: 78.2 kDa
• Gene Summary: Substrate-recognition component of some SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Specifically recognizes p53/TP53, promoting its ubiquitination and degradation (By similarity).[UniProtKB/Swiss-Prot Function]