Lrrn1 (NM_008516) Mouse Tagged ORF Clone

CAT#: MR210244

Lrrn1 (Myc-DDK-tagged) - Mouse leucine rich repeat protein 1, neuronal (Lrrn1)

Product Images

Circular map for MR210244

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lrrn1
• Synonyms: 2810047E21Rik; NLRR-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210244 representing NM_008516
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGATTGAGCACTGGGAAAGCAGCTTGCCAGGTGGTGCTGGGCTTGCTGATAACTTCATTAACTG
  AATCTTCCATACTGACTAGTGAGTGTCCCCAGCTTTGTGTTTGTGAAATTCGTCCTTGGTTTACCCCACA
  GTCCACCTACAGAGAAGCCACCACCGTGGATTGTAATGACCTCCGCCTAACCAGGATCCCTGGCAACCTC
  TCTAGTGACACACAAGTGCTTCTCTTACAGAGCAATAACATCGCCAAGACCGTGGACGAGCTGCAGCAGC
  TCTTCAACTTGACCGAGCTGGATTTCTCCCAGAACAACTTTACAAACATCAAAGAGGTAGGGCTGGCAAA
  CTTGACCCAGCTCACAACCCTGCATCTAGAGGAAAATCAGATTTCAGAGATGACGGATTACTGTCTGCAA
  GACCTCAGCAACCTTCAAGAACTCTACATCAACCACAACCAGATCAGCACAATCTCTGCCAACGCCTTTT
  CTGGCTTGAAAAATCTCTTGAGGCTGCACCTGAATTCCAATAAGTTGAAAGTGATCGATAGCCGCTGGTT
  TGATTCCACTCCCAACCTGGAAATTCTCATGATTGGGGAAAACCCTGTGATTGGAATCCTGGACATGAAC
  TTCAGACCTCTCTCCAACCTGAGAAGCCTAGTGTTAGCAGGAATGTACCTCACTGATGTTCCCGGAAACG
  CCTTGGTAGGCTTGGATAGCCTCGAGAGCCTGTCTTTCTATGACAACAAACTGATCAAAGTCCCTCAACT
  TGCCCTGCAAAAAGTTCCAAATTTGAAATTTTTAGACCTCAATAAAAATCCTATTCACAAAATCCAAGAA
  GGAGACTTTAAAAATATGCTTCGGTTAAAAGAACTGGGAATCAACAACATGGGAGAGCTGGTTTCCGTGG
  ACCGCTATGCCCTGGATAACTTGCCAGAATTGACAAAGCTCGAAGCAACCAATAACCCCAAACTGTCATA
  CATCCACCGTTTGGCTTTCCGTAGTGTGCCGGCTCTAGAAAGCTTAATGTTGAACAACAATGCCTTGAAT
  GCCGTGTACCAAAAAACAGTGGAATCTCTTCCCAATCTGCGTGAGATCAGCATCCACAGCAACCCGCTGA
  GGTGTGACTGTGTCATCCACTGGATCAACTCCAACAAAACCAACATTCGCTTCATGGAGCCCCTCTCCAT
  GTTCTGTGCCATGCCACCGGAATACAGGGGGCAGCAGGTGAAGGAGGTCCTAATCCAGGACTCAAGTGAG
  CAGTGCCTCCCGATGATATCGCACGATACATTCCCTAATCATCTAAACATGGACATTGGTACAACACTTT
  TCCTAGACTGTCGGGCCATGGCTGAGCCAGAACCAGAGATTTACTGGGTCACTCCCATTGGAAATAAGAT
  AACCGTGGAAACTCTTTCAGACAAATACAAGCTGAGTAGTGAGGGCACCTTGGAGATAGCAAACATACAG
  ATTGAAGACTCAGGAAGGTACACCTGCGTTGCCCAGAATGTCCAAGGAGCAGACACTCGAGTGGCAACTA
  TCAAGGTTAATGGAACCCTTCTAGATGGTGCACAGGTGCTGAAAATATATGTCAAACAGACAGAATCTCA
  TTCGATTTTAGTATCTTGGAAAGTTAATTCCAATGTCATGACATCGAACTTGAAATGGTCATCTGCCACT
  ATGAAGATCGACAACCCCCACATAACATACACAGCCAGGGTGCCAGTAGATGTTCATGAATATAACCTAA
  CACATCTGCAGCCTTCTACGGATTATGAAGTGTGTCTCACGGTGTCCAACATCCATCAGCAGACTCAGAA
  GTCATGTGTCAATGTCACAACCAAAACCGCTGCCTTCGCCCTGGACATCTCTGACCATGAAACCAGCACC
  GCCCTTGCTGCGGTGATGGGGTCCATGTTTGCCGTCATCAGTCTAGCATCCATCGCTATTTACATCGCGA
  AAAGGTTTAAGAGGAAAAATTACCACCATTCGCTGAAAAAGTATATGCAAAAAACCTCGTCCATCCCACT
  GAACGAGCTGTACCCCCCACTCATTAACCTCTGGGAAGCTGACAGTGACAAGGACAAAGATGGCTCGGCA
  GACACCAAGCCAACCCAGGTTGACACATCCAGAAGCTATTACATGTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210244 representing NM_008516
  Red=Cloning site Green=Tags(s)
  
  MARLSTGKAACQVVLGLLITSLTESSILTSECPQLCVCEIRPWFTPQSTYREATTVDCNDLRLTRIPGNL
  SSDTQVLLLQSNNIAKTVDELQQLFNLTELDFSQNNFTNIKEVGLANLTQLTTLHLEENQISEMTDYCLQ
  DLSNLQELYINHNQISTISANAFSGLKNLLRLHLNSNKLKVIDSRWFDSTPNLEILMIGENPVIGILDMN
  FRPLSNLRSLVLAGMYLTDVPGNALVGLDSLESLSFYDNKLIKVPQLALQKVPNLKFLDLNKNPIHKIQE
  GDFKNMLRLKELGINNMGELVSVDRYALDNLPELTKLEATNNPKLSYIHRLAFRSVPALESLMLNNNALN
  AVYQKTVESLPNLREISIHSNPLRCDCVIHWINSNKTNIRFMEPLSMFCAMPPEYRGQQVKEVLIQDSSE
  QCLPMISHDTFPNHLNMDIGTTLFLDCRAMAEPEPEIYWVTPIGNKITVETLSDKYKLSSEGTLEIANIQ
  IEDSGRYTCVAQNVQGADTRVATIKVNGTLLDGAQVLKIYVKQTESHSILVSWKVNSNVMTSNLKWSSAT
  MKIDNPHITYTARVPVDVHEYNLTHLQPSTDYEVCLTVSNIHQQTQKSCVNVTTKTAAFALDISDHETST
  ALAAVMGSMFAVISLASIAIYIAKRFKRKNYHHSLKKYMQKTSSIPLNELYPPLINLWEADSDKDKDGSA
  DTKPTQVDTSRSYYMW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008516
• ORF Size: 2148 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008516.4, NP_032542.1
• RefSeq Size: 3756 bp
• RefSeq ORF: 2151 bp
• Locus ID: 16979
• UniProt ID: Q61809
• Cytogenetics: 6 E1
• MW: 81 kDa