Aplp2 (NM_009691) Mouse Tagged ORF Clone

CAT#: MR210097

Aplp2 (Myc-DDK-tagged) - Mouse amyloid beta (A4) precursor-like protein 2 (Aplp2), transcript variant 3

Product Images

Circular map for MR210097

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aplp2
• Synonyms: AI790698; APLP-2; CDEBP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210097 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCACCGGGACCGCGGCCGCTGCGGCCACCGGCAAGCTTCTTGTCCTGCTGCTGCTCGGGCTCA
  CGGCGCCCGCTGCGGCTCTGGCTGGCTACATAGAGGCTCTTGCAGCCAATGCTGGAACAGGCTTCGCTGT
  TGCTGAGCCTCAGATTGCGATGTTCTGTGGGAAGCTGAATATGCATGTGAACATTCAGACTGGCAAATGG
  GAGCCTGACCCAACAGGCACCAAGAGCTGCCTTGGAACAAAGGAGGAGGTTCTTCAGTACTGCCAGGAGA
  TATATCCAGAGCTGCAGATCACAAATGTGATGGAAGCAAACCAGCCAGTCAATATTGATAGTTGGTGCCG
  AAGGGACAAAAGGCAGTGCAAGAGTCACATTGTTATACCATTCAAGTGTCTTGTGGGTGAATTTGTAAGT
  GATGTCCTGCTAGTTCCAGATAACTGCCAGTTTTTCCACCAAGAGCGGATGGAGGTGTGTGAGAAGCACC
  AGCGCTGGCACACGTTAGTCAAGGAGGCATGTCTGACTGAGGGGCTGACCTTATATAGCTATGGCATGCT
  GCTGCCCTGCGGGGTAGACCAGTTCCATGGCACCGAGTATGTGTGCTGCCCTCAGACAAAGACTGTTGAC
  TCGGACTCGACTATGTCCAAAGAAGAGGAGGAAGAGGAAGAGGATGAAGAGGACGAAGAGGAAGACTATG
  ATCTTGATAAAAGTGAATTTCCTACTGAAGCAGATTTGGAAGACTTCACAGAAGCAGCAGCAGATGAGGA
  AGAAGAGGATGAGGAGGAAGGGGAGGAAGTGGTGGAAGACCGTGACTACTACTATGACCCCTTTAAAGGA
  GACGATTACAATGAGGAGAATCCAACCGAACCCAGCAGCGAGGGCACCATTTCAGACAAGGAGATTGTTC
  ACGATGTTAAAGTTCCCCCGACTCCCCTGCCAACCAATGATGTTGATGTGTATTTTGAGACCTCAGCGGA
  TGATAATGAGCACGCCCGCTTCCAGAAGGCTAAGGAACAGCTGGAAATTCGACATCGAAACCGAATGGAC
  AGGGTAAAGAAGGAATGGGAAGAGGCAGAACTTCAAGCCAAGAACCTCCCCAAGACAGAAAGACAGACCC
  TCATCCAGCATTTCCAAGCTATGGTTAAAGCTTTAGAGAAAGAAGCAGCCAGTGAGAAGCAGCAGCTTGT
  GGAAACCCATCTGGCCCGAGTAGAAGCCATGCTGAATGACCGCCGACGCATAGCTCTGGAAAATTACCTG
  GCTGCCCTGCAGTCTGACCCACCTCGGCCACATCGCATTCTTCAAGCTCTTCGTCGTTACGTCCGTGCTG
  AGAACAAAGATCGCCTGCATACCATTCGTCATTACCAACACGTGTTGGCTGTTGACCCAGAAAAGGCCGC
  CCAGATGAAATCCCAGGTGATGACACACCTCCATGTGATTGAAGAAAGAAGGAACCAAAGTCTTTCTCTT
  CTGTACAAAGTTCCTTATGTTGCTCAAGAAATTCAAGAGGAAATTGATGAGCTCCTTCAGGAACAGCGAG
  CGGATATGGACCAATTTACCTCCTCCATCTCAGAGAACCCTGTGGATGTCCGGGTGAGCTCTGAGGAGAG
  TGAGGAGATCCCGCCGTTCCACCCTCTCCATCCCTTCCTATCCTTGTCTGAGAATGAAGGCTCTGGAATG
  GCAGAACAAGACGGGGGACTGATTGGTGCAGAAGAAAAAGTGATTAACAGCAAGAATAAAATGGATGAAA
  ATATGGTCATTGACGAGACTCTGGATGTTAAGGAAATGATTTTCAATGCTGAGAGAGTTGGAGGCCTTGA
  GGAAGAGCCGGAATCGGTGGGACCTTTAAGGGAGGATTTCAGTTTGAGCAGCAATGCCCTTATTGGCTTG
  CTGGTTATCGCAGTGGCCATTGCTACGGTCATCGTTATCAGCCTGGTGATGCTGAGGAAGAGGCAGTACG
  GCACCATCAGCCACGGGATTGTGGAGGTTGACCCAATGCTCACCCCAGAAGAGCGTCACCTGAACAAGAT
  GCAGAACCATGGTTATGAAAACCCAACCTACAAATACCTGGAGCAGATGCAGATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210097 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAATGTAAAAATGKLLVLLLLGLTAPAAALAGYIEALAANAGTGFAVAEPQIAMFCGKLNMHVNIQTGKW
  EPDPTGTKSCLGTKEEVLQYCQEIYPELQITNVMEANQPVNIDSWCRRDKRQCKSHIVIPFKCLVGEFVS
  DVLLVPDNCQFFHQERMEVCEKHQRWHTLVKEACLTEGLTLYSYGMLLPCGVDQFHGTEYVCCPQTKTVD
  SDSTMSKEEEEEEEDEEDEEEDYDLDKSEFPTEADLEDFTEAAADEEEEDEEEGEEVVEDRDYYYDPFKG
  DDYNEENPTEPSSEGTISDKEIVHDVKVPPTPLPTNDVDVYFETSADDNEHARFQKAKEQLEIRHRNRMD
  RVKKEWEEAELQAKNLPKTERQTLIQHFQAMVKALEKEAASEKQQLVETHLARVEAMLNDRRRIALENYL
  AALQSDPPRPHRILQALRRYVRAENKDRLHTIRHYQHVLAVDPEKAAQMKSQVMTHLHVIEERRNQSLSL
  LYKVPYVAQEIQEEIDELLQEQRADMDQFTSSISENPVDVRVSSEESEEIPPFHPLHPFLSLSENEGSGM
  AEQDGGLIGAEEKVINSKNKMDENMVIDETLDVKEMIFNAERVGGLEEEPESVGPLREDFSLSSNALIGL
  LVIAVAIATVIVISLVMLRKRQYGTISHGIVEVDPMLTPEERHLNKMQNHGYENPTYKYLEQMQI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009691
• ORF Size: 2088 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009691.1, NM_009691.2, NP_033821.1
• RefSeq Size: 3490 bp
• RefSeq ORF: 2088 bp
• Locus ID: 11804
• Cytogenetics: 9 16.66 cM
• MW: 79 kDa
• Gene Summary: May play a role in the regulation of hemostasis. The soluble form may have inhibitory properties towards coagulation factors. May interact with cellular G-protein signaling pathways. May bind to the DNA 5'-GTCACATG-3'(CDEI box). Inhibits trypsin, chymotrypsin, plasmin, factor XIA and plasma and glandular kallikrein (By similarity). Modulates the Cu/Zn nitric oxide-catalyzed autodegradation of GPC1 heparan sulfate side chains in fibroblasts.[UniProtKB/Swiss-Prot Function]