Pgghg (NM_145387) Mouse Tagged ORF Clone

CAT#: MR210060

Athl1 (Myc-DDK-tagged) - Mouse ATH1, acid trehalase-like 1 (yeast) (Athl1)

Product Images

Circular map for MR210060

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pgghg
• Synonyms: 5730511L01Rik; Athl1; BC023151
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210060 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGGCTCTGAAGATGACCCGACCATATTCAGTGCCCGTTGTCTGCCCAGTGACCCCAGGCTCTGGG
  CCACTGTAACGAACTCATACCTAGGTACACGTGTGTATCATGACACCATCCATATAAACGGTGTGTACAA
  TGGAGCTGTCGGGGACACTCACCGGGCCAGTCTGCCCAGCCCCCTCAATGTCCAGCTGGAGGCCCCTGCA
  GGGACAGAACAGCTGACAGAGACCTTTACCCTGGACACCAACACAGGTTCCTTCCTCCACACCCTGGAAG
  GCCCCAGCTTCCGGGCTTCCCAGCGGATCTATGCTCACCGTGTGTTACCCCATGTCCTGGTCTTCAGTGT
  GTCCATTGCACGCTTGACCACAGGGAACAAACCCATCACAGTGCCGTTACGGGCAGACTTCTCCCCCGAA
  AGCCCAGACCTGGACCTGCGTGTGGGGCCTGACTTCCAGGGACTCCGGTACCTGCATGGCCACGTACTTA
  ACCCTGAGCAGCCTGGAGAGCCACAGCAGGAAGTGCATATGCTATGGATGCCTGTCCCCCCAGCCTTGAC
  CCTTGGGGAAGAGGAGAAGGACAGGACCTGGGAGTTTCTGACTGTTGTGGGTAGCAGCCAGGCTGAAGCC
  CAGGACTGCTTTGCTGAGGCCCTACAGCTGCAGACCAGGGGTGTTCTGTATACAATCCATGCAGACTCCT
  GGGGCCGGCTCTGGGCAGGCTGTGGCCTGGATGTGGCTGGGCCCCTGGCCCTGCGCCAGGCCCTTCGTGG
  CTCTCTCTACTACCTGTTCAGTGAGCTTCCCCAGCCTGGCACTCAAGGATTCATCAGCCATGGGCTTAGC
  CCCGGAGGACTCTCCAATGGGAGTAAGGAGGAATGCTACTGGGGCCATATCTTCTGGGATCAGGACATCT
  GGATGTTCCCAAATATTTTGATGTTCCACCCAGAAGCCGCCAGGGCCATCCTAGAATACCGAGTCCGTAC
  GCTGGGAGGAGCCCTGAAGAATGGCCAGAACCTGGGCTACCAGGGAGCCAAGTTTGCTTGGGAGAGTGCA
  AGCACTGGCCTGGAGGTCTGCCCTGAGGACATTTATGGGACCCAGGAGATCCATATCAATGGGGCTGTGG
  CCTTGGCCTTTCAGCTGTATTACTATTACACTCAGGACTCGAAGCTCTTCCAAGAGGATGGTGGCTGGGA
  TGTGGTCAGTTCTGTGGCTGAGTTTTGGTGTAGCCGTGTAGAATGGAGCTCCCAGGACAAGATGTACCAC
  CTGAAAGGAGTCATGCCCCCCGATGAGTACCATTCAGGAGTCAACAACTCTGTCTACACCAATGTCCTGG
  TCCAGAACAGCCTGCACTTCGCTGCTGCTTTGGCCAAGGACTTGGGTTTGCCTATCCGAAAACAGTGGCT
  GGAGGTGGCTGATAGGATCAAGATACCATTTGACTCTGAGCAGAACTTCCACCCTGAGTTTGATGGCTAT
  GAGCGTGGAGAAGAGGTGAAGCAGGCAGATGTTGTGCTCCTGGGGTACCCAGTCCCCTTTCCCCTGACAC
  CTGACATCCGAAGGAAAAACTTGGAGACTTATGAGGCCGTGACATCCCCCCAGGGCCCTGCTATGACCTG
  GAGCATGTTTGCTGTGGGCTGGATGGAACTGAGGGACCCCTCAAGGGCTCAGGTCCACCTGAGCAGGAGT
  TTCGCCAATGTCACTGAGCCCTTCAAGGTGTGGACGGAGAATGCAGACGGGTCTGGTGCAGTGAACTTCT
  TGACTGGCATGGGGGGCTTCCTGCAGGCAGCACTCTTCGGGTGCACAGGATTCAGGATCACTGAGGCAGG
  TGTGACCTTTGACCCCCTGTGTCCAGACCTGGTCTCCAGAGTATCTGTCTCTGGTATCTCCTACCTGGGG
  AACAAGATCAACTTCGCCTTTTCCAAGGACTCGGTGACACTTGAGGTCACAGCCCGTGCAGAGCCCTGGG
  CACCCCTACTAGAAGCTGAGCTGTGGCCATCGCTGGCTCATCTTCCCCTGACACCAGGACAGAAGGTCTC
  CTTTCCCCACTCAGCTGGCCGGATACAAAGGTCATCCCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210060 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDGSEDDPTIFSARCLPSDPRLWATVTNSYLGTRVYHDTIHINGVYNGAVGDTHRASLPSPLNVQLEAPA
  GTEQLTETFTLDTNTGSFLHTLEGPSFRASQRIYAHRVLPHVLVFSVSIARLTTGNKPITVPLRADFSPE
  SPDLDLRVGPDFQGLRYLHGHVLNPEQPGEPQQEVHMLWMPVPPALTLGEEEKDRTWEFLTVVGSSQAEA
  QDCFAEALQLQTRGVLYTIHADSWGRLWAGCGLDVAGPLALRQALRGSLYYLFSELPQPGTQGFISHGLS
  PGGLSNGSKEECYWGHIFWDQDIWMFPNILMFHPEAARAILEYRVRTLGGALKNGQNLGYQGAKFAWESA
  STGLEVCPEDIYGTQEIHINGAVALAFQLYYYYTQDSKLFQEDGGWDVVSSVAEFWCSRVEWSSQDKMYH
  LKGVMPPDEYHSGVNNSVYTNVLVQNSLHFAAALAKDLGLPIRKQWLEVADRIKIPFDSEQNFHPEFDGY
  ERGEEVKQADVVLLGYPVPFPLTPDIRRKNLETYEAVTSPQGPAMTWSMFAVGWMELRDPSRAQVHLSRS
  FANVTEPFKVWTENADGSGAVNFLTGMGGFLQAALFGCTGFRITEAGVTFDPLCPDLVSRVSVSGISYLG
  NKINFAFSKDSVTLEVTARAEPWAPLLEAELWPSLAHLPLTPGQKVSFPHSAGRIQRSSP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145387
• ORF Size: 2073 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145387.5
• RefSeq Size: 2975 bp
• RefSeq ORF: 2073 bp
• Locus ID: 212974
• UniProt ID: Q8BP56
• Cytogenetics: 7 F5
• MW: 76.5 kDa
• Gene Summary: Catalyzes the hydrolysis of glucose from the disaccharide unit linked to hydroxylysine residues of collagen and collagen-like proteins.[UniProtKB/Swiss-Prot Function]