Spg20 (NM_144895) Mouse Tagged ORF Clone

CAT#: MR209926

Spg20 (Myc-DDK-tagged) - Mouse spastic paraplegia 20, spartin (Troyer syndrome) homolog (human) (Spg20), transcript variant 1

Product Images

Circular map for MR209926

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Spg20
• Synonyms: AI840044; C79168; mKIAA0610; SPARTIN; TAHCCP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209926 representing NM_144895
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGAGAACCAGAAAATGGAGAACCTGCTGAAATTAAGATCATCAAGGAAGCATACGAGAAGGCCT
  TTATGTTTGTTAATAAAGGACTGAACACGGACGAGTTAGGGCAGAAGGAAGAAGCAAAGAACTATTACAA
  GCAAGGAATAGGACACCTGCTTAGGGGCATCAGCATCGCGGCTGCAGAGCCGGGGCACACAGGCCCTGCG
  TGGGAGGCTGCCAGGCAGATGCAACAGAAAATGAAGGAAACGCTTCAGAACGTCCGCACCAGGCTAGAGA
  TCCTGGAGAAGGGCCTTGCCACTTCTCTACGGAATGACCTTCAGGATGTGCCCAAGCTATACCCAGAGTT
  CCCGCCTAAAGACGCGTGCAAGAAGTCCCCAGAGCAGGAGTCCGTTAGTACAGCGCCCCAGCGTGCTGAA
  GTCGATGGGAGCGCCTCAGCCGCGTGTGCCGGCCCAAGTGGTGCGCCTTCTGCTCTGCCCGTACCATCGC
  CCAGCTGCCCTGCCGAAGCGCCACCAGCCTACAGCCCACAGGCTGCTGAGGGCCACTACACTGTTTCCTA
  TGGAACAGATTCCGGGGAGTTTTCATCCGTGGGAGAAGACTTCTACAGGAACCGTTCCCAGCCGCCCCCT
  CTTGAGACCTTGGGGCTCGATGCAGATGAGTTGATCTTGATACCAAACGGAGTGCAGATCTTTTTTGTAA
  ACCCTGCGGGAGAGGTCAGTGCTCCCTCGTACCCTGGGTATCTGCGGATCGTGCGGTTCTTGGATAATTC
  TCTGGATACAGTTCTGAACCGCCCTCCTGGGTTTCTCCAGGTGTGTGACTGGTTATACCCTCTAGTTCCT
  GATAGGTCACCAGTTCTGAAGTGCACTGTGGGAGCCTACATGTTTCCAGATACCATGTTACAAGCTGCTG
  GGTGCTTCGTAGGGGTTGTTCTTTCATCTGAGCTCCCAGAAGATGACAGAGAGCTGTTTGAGGATCTGTT
  GAGACAGATGTCTGACCTTCGGCTCCAGGCCAACTGGAATAGAGAAGAAGATGAATTCCAAATCCCTGGG
  AGATCAAGTCACCCCTCTGAACCACCGAAGGAAGCTTCTGGCACTGATGTCAGGCAGTCAAGTTCTTCAG
  GTTCCTCAATAGACCAAGGCAGCAAGGATGCCCGCCATAAAGGAAAGCGTGGGAAAAAGACCAAAGATTC
  AAGTGAAGAAGTTAATCTGAGCCAGATTGTGCCCTGTGAGCCGAGTTCAGAAGAAAAATCGAAAGAATTG
  CCTGAATGGAGCGAGAAAGTGGCCCACAACATCCTGTCAGGTGCTTCCTGGGTGAGCTGGGGTTTAGTCA
  AAGGTGCTGAATTTACTGGCAAAGCAATTCAGAAAGGTGCTTCTAAACTCAGAGAGCGCATTCAGCCGGA
  GGAAAAGCCAGTGGAGGTGAGCCCAGCTGTCACCAGGGGTCTCTACATAGCGAAGCAGGCAACCGGAGGA
  GCAGCCAAAGTCAGCCAGCTCCTGGTTGACGGCGTCTGCACAGTAGCGAATTGTGTTGGTAAAGAACTTG
  CTCCACACGTCAAGAAGCACGGAAGCAAACTGGTTCCGGAATCCCTGAAGAGGGACAAAGACGGGAAATC
  TGCCCTGGATGGCGCCATGGTCGTGGCAGCGAGTAGTGTTCAAGGATTCTCAACTGTCTGGCAAGGGTTG
  GAATGTGCAGCTAAATGCATCGTTAACAACGTGTCTGCAGAAACTGTACAGACTGTGAGATACAAGTATG
  GGCACAACGCAGGAGAAGCTACACATAACGCAGTGGATTCAGCTATCAATGTTGGGCTCACTGCCTACAA
  TATTGACAACATCGGCATCAAAGCCATGGTGAAGAAAACTGCCAAGCAGACGGGACACACTCTCCTCGAG
  GACTACCAGATCGTTGAGCGCCCTCAGAGGGAGAGTCAGGGAGGAGCCACAAGCACAGAAGGGAGAAGAG
  ACATAGGAAAGCAGGTGGAGGAAGAGAAGCCAGGGGCAGGAAAGAAGGACAAA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209926 representing NM_144895
  Red=Cloning site Green=Tags(s)
  
  MEREPENGEPAEIKIIKEAYEKAFMFVNKGLNTDELGQKEEAKNYYKQGIGHLLRGISIAAAEPGHTGPA
  WEAARQMQQKMKETLQNVRTRLEILEKGLATSLRNDLQDVPKLYPEFPPKDACKKSPEQESVSTAPQRAE
  VDGSASAACAGPSGAPSALPVPSPSCPAEAPPAYSPQAAEGHYTVSYGTDSGEFSSVGEDFYRNRSQPPP
  LETLGLDADELILIPNGVQIFFVNPAGEVSAPSYPGYLRIVRFLDNSLDTVLNRPPGFLQVCDWLYPLVP
  DRSPVLKCTVGAYMFPDTMLQAAGCFVGVVLSSELPEDDRELFEDLLRQMSDLRLQANWNREEDEFQIPG
  RSSHPSEPPKEASGTDVRQSSSSGSSIDQGSKDARHKGKRGKKTKDSSEEVNLSQIVPCEPSSEEKSKEL
  PEWSEKVAHNILSGASWVSWGLVKGAEFTGKAIQKGASKLRERIQPEEKPVEVSPAVTRGLYIAKQATGG
  AAKVSQLLVDGVCTVANCVGKELAPHVKKHGSKLVPESLKRDKDGKSALDGAMVVAASSVQGFSTVWQGL
  ECAAKCIVNNVSAETVQTVRYKYGHNAGEATHNAVDSAINVGLTAYNIDNIGIKAMVKKTAKQTGHTLLE
  DYQIVERPQRESQGGATSTEGRRDIGKQVEEEKPGAGKKDK
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144895
• ORF Size: 2013 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144895.2, NP_659144.1
• RefSeq Size: 3797 bp
• RefSeq ORF: 2016 bp
• Locus ID: 229285
• UniProt ID: Q8R1X6
• Cytogenetics: 3 C
• MW: 73.1 kDa
• Gene Summary: May be implicated in endosomal trafficking, or microtubule dynamics, or both. Participates in cytokinesis.[UniProtKB/Swiss-Prot Function]