Hdc (NM_008230) Mouse Tagged ORF Clone

CAT#: MR209853

Hdc (Myc-DDK-tagged) - Mouse histidine decarboxylase (Hdc)

Product Images

Circular map for MR209853

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hdc
• Synonyms: AW108189; Hdc-a; Hdc-c; Hdc-e; Hdc-s
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209853 representing NM_008230
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGAGCCCTGTGAATACCGTGAATACCGTGAATACTACCGAGCTAGAGGGAAAGAGATGGTGGATT
  ACATCTCCCAGTACCTGAGCACTGTGCGGGAGAGGCAGGTGACTCCAAATGTGCAGCCTGGATACCTGCG
  AGCCCAGCTACCTGCGAGTGCTCCCGAGGAACCCGACAGCTGGGACAGCATCTTTGGGGACATTGAACGA
  GTCATCATGCCTGGGGTGGTTCACTGGCAGAGCCCCCACATGCACGCCTACTATCCTGCTCTTACCTCAT
  GGCCATCCCTGCTAGGAGACATGCTGGCCGATGCCATTAACTGCTTAGGATTCACGTGGGCATCTAGCCC
  AGCTTGCACAGAGCTGGAGATGAACATCATGGACTGGCTGGCGAAGATGCTGGGGCTCCCGGAGTACTTC
  CTGCACCACCATCCCAGCAGCCGAGGGGGAGGTGTCTTACAGAGCACTGTCAGCGAATCCACTTTAATTG
  CCCTGCTGGCAGCAAGAAAGAACAAAATCCTAGCAATGAAAGCATGCGAGCCCGATGCTAATGAGTCCTC
  CCTGAACGCTCGGCTCGTTGCCTACACCTCTGATCAGGCTCACTCTTCAGTGGAGAAGGCTGGCTTGATT
  TCCCTTGTGAAGATCAGATTTCTACCTGTGGACGACAACTTCTCACTCCGAGGAGAAGCTCTCCAGAAAG
  CTATTGAGGAAGACAAGCAACAGGGCTTGGTGCCTGTGTTTGTCTGTGCAACGTTAGGGACTACTGGAGT
  CTGTGCATTTGACAGGCTGTCAGAACTGGGGCCCATCTGTGCCAGTGAGGGACTGTGGCTCCACGTCGAT
  GCTGCTTATGCAGGCACGGCCTTTCTGTGCCCTGAGCTCCGGGGGTTCCTGGAGGGCATCGAGTACGCTG
  ACTCCTTCACCTTTAACCCTTCCAAGTGGATGATGGTACACTTTGACTGTACTGGATTCTGGGTCAAGGA
  CAAGTACAAGCTGCAGCAGACCTTTAGTGTGAACCCCATCTACCTCCGACATGCCAACTCTGGTGCAGCC
  ACGGACTTCATGCATTGGCAGATCCCCTTGAGCCGGCGCTTTCGCTCCATTAAGCTGTGGTTTGTGATTC
  GGTCCTTCGGGGTGAAGAATCTTCAAGCACATGTCAGACACGGCACAGAAATGGCTAAATACTTTGAATC
  TCTGGTCAGAAGCGACCCTTCCTTCGAAATTCCTGCTAAGAGGCACCTTGGTTTGGTGGTTTTCCGTCTG
  AAGGGTCCTAATTGTCTCACAGAAAGTGTGTTAAAGGAAATAGCCAAAGCTGGCCAGCTCTTTCTCATCC
  CGGCTACTATCCAAGACAAGCTGATCATCCGTTTCACTGTGACATCCCAGTTTACCACCAAGGAGGACAT
  CCTGAGAGATTGGCACCTCATCCAAGAGGCTGCTAACCTTGTCCTGAGCCAGCACTGCACTTCCCAGCCG
  AGCCCTCGGGCCAAGAACGTCATCCCGCCACCGCCAGGGACCAGAGGGCTATCCCTGGAGTCAGTCAGCG
  AGGGAGGAGATGACCCAGCACAGGCCCGGAAGATCATCAAGCAGCCAGGAGCCAGTCTGGCGAGAAGGGA
  AGGCGGCTCTGATCTGGAAACGATGCCGGATCCCTTTGATGATTGCTTCTCGGAAGAGGCCCCCAACACC
  ACCAAGCACAAGCTGTCGTCCTTTCTGTTCAGTTACTTGTCCGTCCAGAACAGGAGGAAGACAACGCGGT
  CCCTCAGCTGCAACAGTGTGCCTATGAGTGCCCAGAAGTCACTCCCCGCAGACGCTTCACTGAAGAATGG
  GGGCTCCTTCCGGGCCAGAATCTTTTCCGGGTTCCCAGAACAAATGATGATGATGAAGAAAGGTGCCTTC
  AAAAAGCTGATCAAGTTCTACAGCGTCCCCAGCTTTCCTGAATGCAGTTCTCAGTGTGCTCGCCAGCTAC
  CGTGCTGCCCCCTGGAGGCCATGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209853 representing NM_008230
  Red=Cloning site Green=Tags(s)
  
  MMEPCEYREYREYYRARGKEMVDYISQYLSTVRERQVTPNVQPGYLRAQLPASAPEEPDSWDSIFGDIER
  VIMPGVVHWQSPHMHAYYPALTSWPSLLGDMLADAINCLGFTWASSPACTELEMNIMDWLAKMLGLPEYF
  LHHHPSSRGGGVLQSTVSESTLIALLAARKNKILAMKACEPDANESSLNARLVAYTSDQAHSSVEKAGLI
  SLVKIRFLPVDDNFSLRGEALQKAIEEDKQQGLVPVFVCATLGTTGVCAFDRLSELGPICASEGLWLHVD
  AAYAGTAFLCPELRGFLEGIEYADSFTFNPSKWMMVHFDCTGFWVKDKYKLQQTFSVNPIYLRHANSGAA
  TDFMHWQIPLSRRFRSIKLWFVIRSFGVKNLQAHVRHGTEMAKYFESLVRSDPSFEIPAKRHLGLVVFRL
  KGPNCLTESVLKEIAKAGQLFLIPATIQDKLIIRFTVTSQFTTKEDILRDWHLIQEAANLVLSQHCTSQP
  SPRAKNVIPPPPGTRGLSLESVSEGGDDPAQARKIIKQPGASLARREGGSDLETMPDPFDDCFSEEAPNT
  TKHKLSSFLFSYLSVQNRRKTTRSLSCNSVPMSAQKSLPADASLKNGGSFRARIFSGFPEQMMMMKKGAF
  KKLIKFYSVPSFPECSSQCARQLPCCPLEAMV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008230
• ORF Size: 1986 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008230.6, NP_032256.3
• RefSeq Size: 2415 bp
• RefSeq ORF: 1989 bp
• Locus ID: 15186
• UniProt ID: P23738
• Cytogenetics: 2 61.76 cM
• MW: 74 kDa
• Gene Summary: Catalyzes the biosynthesis of histamine from histidine.[UniProtKB/Swiss-Prot Function]