Mcam (NM_023061) Mouse Tagged ORF Clone

CAT#: MR209740

Mcam (Myc-DDK-tagged) - Mouse melanoma cell adhesion molecule (Mcam)

Product Images

Circular map for MR209740

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mcam
• Synonyms: 1-gicerin; AV025631; CD146; CD149; Muc18; s-endo; s-gicerin
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209740 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCTGCCCAAACTGGTGTGCGTCTTCTTGTTCGCTGCCTGCTGCTGCTGTCGCCGTGCCGCGGGTG
  TGCCAGGAGAGGAAAAGCAGCCAGTACCCACGCCCGACCTGGTGGAGGCAGAAGTGGGCAGCACAGCCCT
  TCTCAAGTGTGGCCCCTCACGGGCCTCAGGCAACTTCAGCCAAGTGGACTGGTTTTTGATTCACAAGGAG
  AGGCAGATACTGATTTTCCGTGTGCACCAAGGCAAGGGCCAGCGGGAACCTGGTGAATATGAGCACCGCC
  TTAGCCTCCAAGACTCGGTGGCTACTCTGGCCCTGAGTCACGTCACTCCCCATGATGAGCGAATGTTCCT
  GTGTAAGAGCAAGCGACCACGGCTCCAGGATCACTACGTTGAGCTTCAGGTCTTCAAAGCCCCAGAGGAA
  CCAACTATTCAAGCCAATGTCGTGGGCATCCATGTGGACAGGCAAGAGCTCAGGGAGGTTGCTACCTGTG
  TGGGGAGAAACGGCTACCCCATTCCTCAAGTCCTATGGTACAAGAACAGTCTGCCCTTGCAAGAGGAGGA
  GAACCGAGTTCATATCCAGTCATCACAGATTGTCGAGTCCAGTGGCTTGTACACCTTGAAGAGTGTTCTG
  AGTGCACGCCTAGTTAAGGAAGACAAAGATGCCCAGTTTTACTGTGAACTCAGCTACCGGCTACCCAGTG
  GGAACCACATGAAGGAATCTAAGGAGGTCACTGTCCCTGTTTTCTACCCTGCAGAAAAAGTGTGGGTGGA
  GGTAGAGCCTGTGGGGCTGCTGAAGGAAGGGGATCATGTGACAATCAGGTGTCTGACAGATGGCAACCCT
  CAACCCCACTTCACTATCAACAAGAAGGACCCCAGCACTGGGGAGATGGAAGAGGAGAGCACCGATGAAA
  ATGGGCTCCTGTCCTTGGAGCCTGCCGAAAAGCACCATAGCGGGCTCTACCAGTGTCAGAGTCTGGACCT
  GGAAACTACCATCACACTGTCAAGTGACCCCCTGGAGCTGCTGGTGAACTATGTGTCTGATGTTCAAGTG
  AATCCAACTGCCCCTGAAGTCCAGGAAGGTGAGAGCCTCACGCTGACCTGCGAGGCAGAAAGTAACCAGG
  ACCTTGAGTTTGAGTGGCTGAGAGACAAGACAGGCCAGCTGCTGGGAAAGGGTCCCGTCCTCCAGCTAAA
  CAACGTGAGACGGGAAGCAGGGGGACGGTATCTCTGCATGGCATCTGTCCCCAGAGTTCCTGGCTTGAAT
  CGTACCCAGCTGGTCAGCGTGGGCATTTTTGGGTCCCCATGGATGGCATTAAAGGAGAGGAAGGTGTGGG
  TGCAAGAGAATGCAGTGCTGAATCTGTCTTGTGAGGCTTCAGGACATCCTCAGCCCACCATCTCCTGGAA
  TGTCAATGGTTCGGCAACTGAATGGAACCCAGATCCACAGACAGTAGTGAGCACCTTGAATGTCCTTGTG
  ACGCCAGAGCTTCTGGAGACAGGTGCAGAGTGTACAGCCTCCAACTCCCTGGGCTCAAACACCACCACCA
  TTGTTCTGAAGCTGGTCACTTTAACCACCCTCATACCTGACTCCAGCCAAACCACTGGCCTCAGCACCCT
  CACAGTCAGTCCTCACACCAGAGCCAACAGCACCTCCACAGAGAAAAAGCTGCCACAGCCAGAGAGCAAA
  GGTGTGGTCATCGTGGCTGTGATAGTGTGTACCTTGGTGCTTGCTGTGCTGGGTGCTGCTCTCTATTTCT
  TCTACAAGAAGGGCAAGCTGCCATGTGGACGCTCGGGAAAACAGGAGATCACGCTGCCCCCGACTCGTAA
  GAGTGAATTTGTAGTTGAAGTTAAGTCAGATAAGCTCCCAGAAGAGATGGCTCTCCTTCAGGGCAGCAAC
  GGTGACAAGAGGGCTCCAGGAGACCAGGGAGAGAAATACATCGATCTGAGGCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209740 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGLPKLVCVFLFAACCCCRRAAGVPGEEKQPVPTPDLVEAEVGSTALLKCGPSRASGNFSQVDWFLIHKE
  RQILIFRVHQGKGQREPGEYEHRLSLQDSVATLALSHVTPHDERMFLCKSKRPRLQDHYVELQVFKAPEE
  PTIQANVVGIHVDRQELREVATCVGRNGYPIPQVLWYKNSLPLQEEENRVHIQSSQIVESSGLYTLKSVL
  SARLVKEDKDAQFYCELSYRLPSGNHMKESKEVTVPVFYPAEKVWVEVEPVGLLKEGDHVTIRCLTDGNP
  QPHFTINKKDPSTGEMEEESTDENGLLSLEPAEKHHSGLYQCQSLDLETTITLSSDPLELLVNYVSDVQV
  NPTAPEVQEGESLTLTCEAESNQDLEFEWLRDKTGQLLGKGPVLQLNNVRREAGGRYLCMASVPRVPGLN
  RTQLVSVGIFGSPWMALKERKVWVQENAVLNLSCEASGHPQPTISWNVNGSATEWNPDPQTVVSTLNVLV
  TPELLETGAECTASNSLGSNTTTIVLKLVTLTTLIPDSSQTTGLSTLTVSPHTRANSTSTEKKLPQPESK
  GVVIVAVIVCTLVLAVLGAALYFFYKKGKLPCGRSGKQEITLPPTRKSEFVVEVKSDKLPEEMALLQGSN
  GDKRAPGDQGEKYIDLRH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023061
• ORF Size: 1947 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023061.2
• RefSeq Size: 2892 bp
• RefSeq ORF: 1947 bp
• Locus ID: 84004
• UniProt ID: Q8R2Y2
• Cytogenetics: 9 A5.1
• MW: 71.5 kDa
• Gene Summary: Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration (By similarity).[UniProtKB/Swiss-Prot Function]