Tnip1 (NM_021327) Mouse Tagged ORF Clone

CAT#: MR209733

Tnip1 (Myc-DDK-tagged) - Mouse TNFAIP3 interacting protein 1 (Tnip1), transcript variant 1

Product Images

Circular map for MR209733

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tnip1
• Synonyms: ABIN; ABIN-1; ABIN1; AU018810; Naf1; Nef; VAN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209733 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGGAGAGGACCCTACCGGATCTACGACCCAGGGGGCAGCACGCCTCTGGGAGAGGTGTCCGCAG
  CTTTTGAACGTCTAGTGGAGGAGAATACTCGGCTGAAGGGAAAAATGCAAGGGATAAAGATGTTAGGGGA
  GCTTCTGGAGGAGTCTCAGATGGAAGCGTCCAGACTCCGGCAGAAGGCAGAGGAGCTGGTCAAGGACAGC
  GAGCTGTCACCACCGACATCTGCCCCCTCCTTGGTCTCCTTTGATGACCTGGCTGAGCTCACAGGACAGG
  ATACAAAGGTCCAGGTACATCCTGCTACCAGCACTGCCGCCACCACCACCGCCACCGCCACCACGGGAAA
  CTCCATGGAGAAGCCCGAGCCAGCCTCCAAATCTCCGTCCAATGGCGCCTCCTCGGACTTTGAAGTGGTC
  CCTACTGAGGAGCAGAATTCACCCGAAACTGGCAGCCACCCTACGAACATGATGGACCTGGGGCCCCCAC
  CCCCAGAGGACAGCAACCTGAAGCTCCACCTGCAGCGCCTGGAGACCACCCTTAGCGTGTGTGCAGAGGA
  GCCAGACCACAGCCAGCTCTTCACCCACCTGGGCCGCATGGCCCTCGAGTTCAACAGGTTGGCCTCCAAA
  GTGCATAAAAATGAGCAGCGCACCTCCATCCTGCAGACCTTATGTGAGCAGCTGCGCCAGGAGAATGAAG
  CCCTGAAGGCCAAGCTGGACAAGGGCCTGGAACAGCGGGATCTGGCTGCTGAGAGGCTGCGGGAGGAAAA
  CACAGAGCTCAAGAAACTGTTGATGAACAGCAGCTGCAAAGAGGGACTCTGTGGGCAGCCCAGCTCCCCA
  AAGCCAGAGGGTGCTGGCAAGAAGGGCGTGGCTGGACAGCAGCAGGCCAGTGTGATGGCGAGTAAAGTCC
  CTGAAGCGGGGGTCTTTGGAGCAGCTGAGAAGAAGGTGAAGTTGCTAGAACAGCAACGCATGGAGCTGCT
  GGAAGTGAACAAGCAGTGGGACCAGCATTTCCGGTCCATGAAGCAGCAGTATGAGCAGAAGATCACAGAG
  CTTCGCCAGAAGCTGGTGGACCTGCAGAAACAGGTAACTGAGCTGGAGGCCGAACGGGAGCAGAAGCAGC
  GTGACTTTGACCGGAAACTCCTCCTGGCCAAATCGAAGATAGAGATGGAAGAGACCGACAAGGAGCAGCT
  GACAGCAGAGGCCAAGGAACTGCGCCAGAAGGTCAGGTACCTACAGGATCAGCTGAGCCCGCTCACAAGG
  CAACGAGAATACCAGGAGAAGGAGATCCAGCGGCTCAATAAGGCCCTGGAGGAGGCCCTCAGCATCCAGG
  CCTCTCCATCATCTCCGCCTGCAGCTTTTGGGAGTCCTGAAGGCGTTGGGGGCCATCTGAGGAAGCAGGA
  ACTGGTGACACAGAATGAGTTGCTGAAACAGCAGGTAAAGATCTTTGAAGAGGACTTCCAGAGGGAACGG
  AGTGACCGTGAACGCATGAATGAAGAGAAGGAGGAGCTGAAGAAGCAAGTAGAGAAGCTGCAGGCCCAGG
  TCACCCTGACTAATGCCCAGCTCAAAACTCTCAAAGAGGAGGAGAAGGCCAAGGAAGTCCTCAAACAGCA
  GAAGAGGAAAGCAAAGGCTTCGGGAGAGCGCTACCACATGGAACCCCACCCTGAGCACGTCTGCGGCGCC
  TATCCCTATGCCTACCCACCCATGCCAGCCATGGTACCTCACCATGCCTACAAGGACTGGTCCCAGATCC
  GATACCCTCCACCCCCTGTGCCCATGGAGCACCCGCCCCCACACCCCAACTCTCGCCTCTTCCATCTGCC
  GGAGTACACCTGGCGTCCACCCTGTGCAGGCATTCGGAATCAGAGCTCTCAAGTGATGGACCCGCCCCCA
  GACAGGCCTGCAGAGCCAGAGTCTGCAGACAATGACTGTGATGGGCCCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209733 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEGRGPYRIYDPGGSTPLGEVSAAFERLVEENTRLKGKMQGIKMLGELLEESQMEASRLRQKAEELVKDS
  ELSPPTSAPSLVSFDDLAELTGQDTKVQVHPATSTAATTTATATTGNSMEKPEPASKSPSNGASSDFEVV
  PTEEQNSPETGSHPTNMMDLGPPPPEDSNLKLHLQRLETTLSVCAEEPDHSQLFTHLGRMALEFNRLASK
  VHKNEQRTSILQTLCEQLRQENEALKAKLDKGLEQRDLAAERLREENTELKKLLMNSSCKEGLCGQPSSP
  KPEGAGKKGVAGQQQASVMASKVPEAGVFGAAEKKVKLLEQQRMELLEVNKQWDQHFRSMKQQYEQKITE
  LRQKLVDLQKQVTELEAEREQKQRDFDRKLLLAKSKIEMEETDKEQLTAEAKELRQKVRYLQDQLSPLTR
  QREYQEKEIQRLNKALEEALSIQASPSSPPAAFGSPEGVGGHLRKQELVTQNELLKQQVKIFEEDFQRER
  SDRERMNEEKEELKKQVEKLQAQVTLTNAQLKTLKEEEKAKEVLKQQKRKAKASGERYHMEPHPEHVCGA
  YPYAYPPMPAMVPHHAYKDWSQIRYPPPPVPMEHPPPHPNSRLFHLPEYTWRPPCAGIRNQSSQVMDPPP
  DRPAEPESADNDCDGPQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021327
• ORF Size: 1944 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021327.1, NM_021327.2, NM_021327.3, NM_021327.4, NP_067302.1
• RefSeq Size: 2838 bp
• RefSeq ORF: 1944 bp
• Locus ID: 57783
• Cytogenetics: 11 B1.3
• MW: 73.1 kDa
• Gene Summary: Inhibits NF-kappa-B activation and TNF-induced NF-kappa-B-dependent gene expression by regulating A20/TNFAIP3-mediated deubiquitination of IKBKG; proposed to link A20/TNFAIP3 to ubiquitinated IKBKG. Involved in regulation of EGF-induced ERK1/ERK2 signaling pathway; blocks MAPK3/MAPK1 nuclear translocation and MAPK1-dependent transcription. Increases cell surface CD4(T4) antigen expression. Involved in the anti-inflammatory response of macrophages and positively regulates TLR-induced activation of CEBPB. Involved in the prevention of autoimmunity; this function implicates binding to polyubiquitin. Involved in leukocyte integrin activation during inflammation; this function is mediated by association with SELPLG and dependent on phosphorylation by SRC-family kinases.[UniProtKB/Swiss-Prot Function]