Pex5 (NM_008995) Mouse Tagged ORF Clone

CAT#: MR209684

Pex5 (Myc-DDK-tagged) - Mouse peroxisomal biogenesis factor 5 (Pex5), transcript variant 1

Product Images

Circular map for MR209684

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pex5
• Synonyms: AW212715; ESTM1; PTS1R; Pxr1; X83306
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209684 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAATGCGGGAGCTGGTGGAGGGCGAATGTGGGGGTGCCAACCCGCTGATGAAGCTGGCCACCCATT
  TCACCCAGGACAAGGCCCTTCGGCAGGAGGGACTGAGGCCTGGCCCCTGGCCTCCGGGAGCCTCAGCAGC
  AGAGACCGTTTCCAAGCCTTTGGGAGTAGGAACTGAAGACGAGTTGGTGTCAGAATTCCTGCAGGACCAG
  AATGCAACTCTTGTATCCCGAGCCCCTCAGACCTTCAAGATGGATGACCTCCTGGCAGAGATGCAGGAGA
  TTGAACAGTCAAACTTTCGCCAGGCTCCTCAGAGAGCCCCTGGTGTGGCAGATTTGGCCTTGTCTGAGAA
  TTGGGCCCAGGAGTTTCTTGCAGCTGGAGATGCTGTGGATGTAGCTCAAGATTATAATGAGACCGACTGG
  TCCCAAGAATTCATCGCCGAAGTTACAGACCCACTGTCCGTGTCCCCTGCCCGATGGGCTGAGGAGTATC
  TGGAGCAGTCTGAGGAGAAGCTGTGGTTGGGAGACCAGGAAGGATCCTCTACCGCTGATCGATGGTACGA
  TGAATATCATCCTGAGGAGGACCTGCAGCACACAGCCAGTGACTTTGTGTCCAAGGTGGACGACCCCAAA
  TTGGCTAACTCTGAGTTCCTGAAATTCGTGCGGCAGATTGGCGAGGGGCAGGTGTCCCTGGAGTCGGCTG
  CAGGGTCGGGCGGAGCTCAGGCAGAACAGTGGGCAGCAGAGTTTATACAGCAGCAGGGCACGTCAGAGGC
  CTGGGTTGATCAGTTTACAAGACCAGGAAACAAAATAGCTGCCCTTCAGGTGGAATTTGAACGAGCCAAG
  TCAGCTATAGAGTCTGATGTCGATTTCTGGGACAAGTTACAGGCAGAGTTGGAGGAGATGGCCAAGCGGG
  ATGCCGAGGCGCACCCCTGGCTTTCTGACTATGATGACCTCACATCCGCTTCCTATGACAAGGGTTACCA
  GTTTGAGGAGGAGAATCCCTTGCGTGACCACCCTCAGCCTTTTGAAGAAGGGCTGCATCGACTGGAGGAG
  GGAGACCTGCCCAATGCTGTGTTGCTCTTCGAGGCTGCTGTGCAGCAGGATCCTAAGCACATGGAAGCTT
  GGCAGTACCTGGGCACCACCCAGGCCGAGAATGAGCAAGAGCTCTTGGCTATCAGTGCCCTGCGGAGGTG
  TCTGGAACTAAAGCCAGATAACCGGACAGCACTGATGGCGCTGGCTGTGAGCTTTACCAATGAGTCCCTG
  CAGCGCCAGGCCTGTGAGACGTTGAGGGACTGGCTGCGCTACTCACCGGCCTACGCTCATCTGGTGGCTC
  CTGGGGAAGAAGGGGCGACCGGGGCAGGCCCCAGCAAGCGCATCTTGGGATCTCTGTTGTCTGACTCCCT
  GTTTCTTGAAGTAAAAGATCTCTTCCTGGCGGCTGTGCGTCTGGACCCTACATCCATTGACCCTGATGTG
  CAGTGTGGCCTGGGAGTCCTCTTCAACCTGAGCGGGGAGTACGACAAGGCTGTGGACTGTTTCACAGCTG
  CCCTCAGTGTTCGCCCCAATGACTATTTGATGTGGAACAAGCTTGGGGCCACCCTGGCCAATGGGAACCA
  GAGTGAAGAAGCAGTGGCTGCATACCGCCGAGCCCTTGAACTACAGCCTGGGTATATTCGGTCCCGGTAT
  AACCTAGGCATCAGCTGCATCAACCTGGGAGCTCACCGTGAGGCTGTGGAGCACTTTCTGGAGGCTCTGA
  ACATGCAGAGGAAGAGCCGGGGCCCTCGGGGTGAGGGGGGCGCCATGTCTGAGAACATCTGGAGTACCCT
  GCGCTTGGCGTTATCCATGTTAGGCCAGAGCGATGCCTATGGGGCAGCCGATGCTCGGGATCTGTCTGCC
  CTCCTAGCTATGTTTGGCCTGCCCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209684 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAMRELVEGECGGANPLMKLATHFTQDKALRQEGLRPGPWPPGASAAETVSKPLGVGTEDELVSEFLQDQ
  NATLVSRAPQTFKMDDLLAEMQEIEQSNFRQAPQRAPGVADLALSENWAQEFLAAGDAVDVAQDYNETDW
  SQEFIAEVTDPLSVSPARWAEEYLEQSEEKLWLGDQEGSSTADRWYDEYHPEEDLQHTASDFVSKVDDPK
  LANSEFLKFVRQIGEGQVSLESAAGSGGAQAEQWAAEFIQQQGTSEAWVDQFTRPGNKIAALQVEFERAK
  SAIESDVDFWDKLQAELEEMAKRDAEAHPWLSDYDDLTSASYDKGYQFEEENPLRDHPQPFEEGLHRLEE
  GDLPNAVLLFEAAVQQDPKHMEAWQYLGTTQAENEQELLAISALRRCLELKPDNRTALMALAVSFTNESL
  QRQACETLRDWLRYSPAYAHLVAPGEEGATGAGPSKRILGSLLSDSLFLEVKDLFLAAVRLDPTSIDPDV
  QCGLGVLFNLSGEYDKAVDCFTAALSVRPNDYLMWNKLGATLANGNQSEEAVAAYRRALELQPGYIRSRY
  NLGISCINLGAHREAVEHFLEALNMQRKSRGPRGEGGAMSENIWSTLRLALSMLGQSDAYGAADARDLSA
  LLAMFGLPQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008995
• ORF Size: 1920 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008995.2, NP_033021.2
• RefSeq Size: 3148 bp
• RefSeq ORF: 1920 bp
• Locus ID: 19305
• UniProt ID: O09012
• Cytogenetics: 6 59.15 cM
• MW: 70.8 kDa
• Gene Summary: Binds to the C-terminal PTS1-type tripeptide peroxisomal targeting signal (SKL-type) and plays an essential role in peroxisomal protein import.[UniProtKB/Swiss-Prot Function]