Rtn4 (BC032192) Mouse Tagged ORF Clone

CAT#: MR209681

Rtn4 (Myc-DDK-tagged) - Mouse reticulon 4 (cDNA clone MGC:38204 IMAGE:5323152)

Product Images

Circular map for MR209681

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rtn4
• Synonyms: ASY, NOGO, NSP-CL, C130026I10Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209681 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGAAGGTCTAACGCCAGATTTAGTTCAGGAAGCATGTGAAAGTGAACTGAACGAAGCCACAGGTA
  CAAAGATTGCTTATGAAACAAAAGTGGACTTGGTCCAGACATCAGAAGCTATACAAGAGTCAATTTACCC
  CACAGCACAGCTTTGCCCCTCATTTGAGGAAGCTGAAGCAACTCCGTCACCAGTTTTGCCTGATATTGTT
  ATGGAAGCGCCATTAAATTCTCTCCTTCCAAGCACTGGTGCTTCTGTAGCGCAGCCCAGTGCATCCCCAC
  TAGAAGTACCGTCTCCAGTTAGTTATGACGGTATAAAGCTTGAGCCTGAAAATCCCCCACCATATGAAGA
  AGCCATGAGTGTAGCACTAAAAACATCGGACGCAAAGGAAGAAATTAAAGAGCCTGAAAGTTTTAATGCA
  GCTGCTCAGGAAGCAGAAGCTCCTTATATATCCATTGCATGTGATTTAATTAAAGAAACAAAGCTCTCCA
  CTGAGCCAAGTCCAGAGTTCTCTAATTATTCAGAAATAGCAAAATTTGAGAAGTCGGTGCCTGATCACTG
  TGAGCTCGTGGATGATTCCTCACCCGAATCTGAACCAGTTGACTTATTTAGTGATGATTCAATTCCTGAA
  GTCCCACAAACACAAGAGGAGGCTGTGATGCTAATGAAGGAGAGTCTCACTGAAGTGTCTGAGACAGTAA
  CACAACACAAACATAAGGAGAGACTTAGTGCTTCACCTCAGGAGGTAGGAAAGCCATATTTAGAGTCTTT
  TCAGCCCAATTTACATATTACAAAAGATGCTGCATCTAATGAAATTCCAACATTGACCAAAAAGGAGACA
  ATTTCTTTGCAAATGGAAGAGTTTAATACTGCAATTTATTCCAATGATGACTTACTTTCTTCTAAGGAAG
  ACAAAATGAAAGAAAGTGAAACATTTTCCGATTCATCTCCCATTGAGATAATAGATGAGTTTCCCACATT
  TGTCAGTGCTAAAGATGATTCTCCTAAGGAGTACACTGACCTAGAAGTATCCAACAAAAGTGAAATTGCT
  AATGTCCAGAGCGGGGCCAATTCGTTGCCTTGCTCAGAATTGCCCTGTGACCTTTCTTTCAAGAATACAT
  ATCCTAAAGATGAAGCACATGTCTCAGATGAATTCTCCAAAAGTAGGTCCAGTGTATCTAAGGTGCCCTT
  ATTGCTTCCAAATGTTTCTGCTTTGGAATCTCAAATAGAAATGGGCAACATAGTTAAACCCAAAGTACTT
  ACGAAAGAAGCAGAGGAAAAACTTCCTTCTGATACAGAGAAAGAGGACAGATCCCTGACAGCTGTATTGT
  CAGCAGAGCTGAATAAAACTTCAGTTGTTGACCTCCTGTACTGGAGAGACATTAAGAAGACTGGAGTGGT
  GTTTGGTGCCAGCTTATTCCTGCTGCTGTCTCTGACAGTGTTCAGCATTGTCAGTGTAACGGCCTACATT
  GCCTTGGCCCTGCTCTCTGTGACTATCAGCTTTAGGATATATAAGGGTGTGATCCAAGCTATCCAGAAAT
  CAGATGAAGGCCACCCATTCAGGGCATATTTGGAATCTGAAGTTGCCATATCAGAGGAATTGGTTCAGAA
  ATATAGTAATTCTGCTCTTGGTCATGTGAACAGCACAATAAAAGAATTGAGGCGTCTCTTCTTAGTTGAT
  GATTTAGTTGATTCCCTGAAGTTTGCAGTGTTGATGTGGGTATTTACTTACGTTGGTGCCTTGTTCAATG
  GTTTGACACTACTGATTTTAGCTCTGATCTCACTCTTCAGTATTCCTGTTATATATGAACGGCATCAGGC
  GCAGATAGATCATTATCTAGGACTTGCAAACAAGAGCGTTAAGGATGCCATGGCCAAAATCCAAGCAAAA
  ATCCCTGGATTGAAGCGCAAAGCAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209681 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPEGLTPDLVQEACESELNEATGTKIAYETKVDLVQTSEAIQESIYPTAQLCPSFEEAEATPSPVLPDIV
  MEAPLNSLLPSTGASVAQPSASPLEVPSPVSYDGIKLEPENPPPYEEAMSVALKTSDAKEEIKEPESFNA
  AAQEAEAPYISIACDLIKETKLSTEPSPEFSNYSEIAKFEKSVPDHCELVDDSSPESEPVDLFSDDSIPE
  VPQTQEEAVMLMKESLTEVSETVTQHKHKERLSASPQEVGKPYLESFQPNLHITKDAASNEIPTLTKKET
  ISLQMEEFNTAIYSNDDLLSSKEDKMKESETFSDSSPIEIIDEFPTFVSAKDDSPKEYTDLEVSNKSEIA
  NVQSGANSLPCSELPCDLSFKNTYPKDEAHVSDEFSKSRSSVSKVPLLLPNVSALESQIEMGNIVKPKVL
  TKEAEEKLPSDTEKEDRSLTAVLSAELNKTSVVDLLYWRDIKKTGVVFGASLFLLLSLTVFSIVSVTAYI
  ALALLSVTISFRIYKGVIQAIQKSDEGHPFRAYLESEVAISEELVQKYSNSALGHVNSTIKELRRLFLVD
  DLVDSLKFAVLMWVFTYVGALFNGLTLLILALISLFSIPVIYERHQAQIDHYLGLANKSVKDAMAKIQAK
  IPGLKRKAE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC032192
• ORF Size: 1917 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC032192, AAH32192
• RefSeq Size: 2958 bp
• RefSeq ORF: 1919 bp
• Locus ID: 68585
• Cytogenetics: 11 A3.3
• MW: 70.3 kDa
• Gene Summary: Required to induce the formation and stabilization of endoplasmic reticulum (ER) tubules. They regulate membrane morphogenesis in the ER by promoting tubular ER production. They influence nuclear envelope expansion, nuclear pore complex formation and proper localization of inner nuclear membrane proteins. However each isoform have specific functions mainly depending on their tissue expression specificities.[UniProtKB/Swiss-Prot Function]