Msln (NM_018857) Mouse Tagged ORF Clone

CAT#: MR209539

Msln (Myc-DDK-tagged) - Mouse mesothelin (Msln)

Product Images

Circular map for MR209539

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Msln
• Synonyms: MPF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209539 representing NM_018857
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTTGCCAACAGCTCGACCCCTGCTGGGGTCCTGTGGAAGTCCCATCTGCAGCCGAAGCTTCCTAC
  TGCTTCTCCTTAGTCTTGGGTGGATACCACGTCTGCAGACCCAGACTACAAAGACAAGCCAGGAGGCCAC
  ACTCCTCCATGCTGTGAACGGTGCCGCTGACTTTGCCAGTCTCCCCACAGGCCTCTTTCTTGGCCTCACA
  TGTGAGGAGGTATCTGACCTGAGCATGGAACAAGCCAAGGGGCTGGCTATGGCTGTAAGACAGAAGAACA
  TTACACTCCGGGGACATCAGCTGCGTTGTCTGGCACGTCGCCTTCCTAGGCACCTCACCGACGAGGAACT
  GAATGCTCTTCCACTGGACCTGCTGCTCTTCCTCAACCCAGCCATGTTTCCAGGGCAACAGGCTTGTGCC
  CACTTCTTCTCCCTCATCTCTAAAGCCAATGTGGATGTACTCCCACGGAGGTCTCTGGAGCGCCAGAGGC
  TGCTGATGGAGGCTCTGAAGTGCCAGGGCGTGTATGGATTTCAAGTGAGTGAGGCAGATGTGCGGGCTCT
  CGGAGGCCTGGCCTGTGACCTGCCTGGGAAATTTGTGGCCAGATCTTCCGAAGTTCTCCTCCCCTGGCTG
  GCAGGATGCCAAGGACCCCTGGACCAGAGCCAGGAAAAGGCAGTCAGGGAGGTTCTGAGGAGTGGAAGAA
  CCCAATATGGCCCCCCATCGAAGTGGTCAGTCTCCACCCTGGATGCCCTGCAGAGCTTGGTAGCAGTGTT
  GGATGAGTCCATCGTCCAGAGCATCCCCAAGGATGTCAAAGCTGAATGGCTGCAACACATCTCCAGAGAC
  CCCTCCAGGCTGGGGTCTAAGCTGACCGTCATACACCCAAGGTTCCGACGGGATGCAGAACAGAAAGCCT
  GCCCTCCAGGGAAGGAGCCCTACAAGGTGGATGAAGACCTCATCTTCTACCAGAATTGGGAGCTGGAGGC
  TTGTGTAGATGGCACCATGCTGGCCAGACAAATGGACCTTGTGAACGAGATTCCCTTCACCTATGAGCAG
  CTCAGTATCTTTAAGCACAAACTGGACAAGACCTACCCACAAGGCTATCCTGAGTCCCTGATCCAGCAGC
  TGGGTCACTTCTTCAGATATGTTAGCCCTGAAGACATCCACCAGTGGAATGTGACCTCACCAGACACAGT
  GAAAACTCTGCTCAAAGTCAGCAAAGGACAAAAGATGAATGCTCAGGCGATTGCCTTGGTCGCCTGCTAT
  CTTCGGGGAGGAGGCCAGCTGGACGAGGATATGGTAAAAGCCCTGGGCGACATCCCGTTAAGCTATCTAT
  GTGACTTCAGCCCCCAGGATCTGCACTCGGTACCCTCCAGTGTCATGTGGCTGGTTGGGCCCCAAGACCT
  GGACAAGTGCAGCCAGAGGCATCTGGGTCTCCTCTACCAGAAGGCCTGCTCAGCCTTCCAGAATGTGAGC
  GGCCTAGAATACTTTGAGAAAATCAAGACATTCCTGGGTGGGGCCTCCGTGAAGGACCTGCGGGCCCTCA
  GCCAGCACAATGTGAGCATGGACATAGCCACTTTCAAGAGGCTGCAGGTGGATTCCCTGGTGGGGCTGAG
  TGTGGCTGAGGTACAGAAACTTCTGGGGCCAAACATTGTGGACCTGAAGACCGAGGAGGATAAAAGCCCT
  GTCCGTGACTGGCTGTTCCGGCAGCATCAGAAAGACCTAGACAGGCTGGGTTTGGGACTTCAGGGTGGCA
  TCCCCAATGGCTACCTGGTCCTGGACTTCAATGTCCGAGAGGCCTTCTCCAGCAGAGCCTCACTCCTTGG
  GCCAGGATTTGTATTAATATGGATTCCAGCTCTGCTCCCAGCTTTAAGGCTGAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209539 representing NM_018857
  Red=Cloning site Green=Tags(s)
  
  MALPTARPLLGSCGSPICSRSFLLLLLSLGWIPRLQTQTTKTSQEATLLHAVNGAADFASLPTGLFLGLT
  CEEVSDLSMEQAKGLAMAVRQKNITLRGHQLRCLARRLPRHLTDEELNALPLDLLLFLNPAMFPGQQACA
  HFFSLISKANVDVLPRRSLERQRLLMEALKCQGVYGFQVSEADVRALGGLACDLPGKFVARSSEVLLPWL
  AGCQGPLDQSQEKAVREVLRSGRTQYGPPSKWSVSTLDALQSLVAVLDESIVQSIPKDVKAEWLQHISRD
  PSRLGSKLTVIHPRFRRDAEQKACPPGKEPYKVDEDLIFYQNWELEACVDGTMLARQMDLVNEIPFTYEQ
  LSIFKHKLDKTYPQGYPESLIQQLGHFFRYVSPEDIHQWNVTSPDTVKTLLKVSKGQKMNAQAIALVACY
  LRGGGQLDEDMVKALGDIPLSYLCDFSPQDLHSVPSSVMWLVGPQDLDKCSQRHLGLLYQKACSAFQNVS
  GLEYFEKIKTFLGGASVKDLRALSQHNVSMDIATFKRLQVDSLVGLSVAEVQKLLGPNIVDLKTEEDKSP
  VRDWLFRQHQKDLDRLGLGLQGGIPNGYLVLDFNVREAFSSRASLLGPGFVLIWIPALLPALRLS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018857
• ORF Size: 1875 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018857.2
• RefSeq Size: 2135 bp
• RefSeq ORF: 1878 bp
• Locus ID: 56047
• UniProt ID: Q61468
• Cytogenetics: 17 A3.3
• MW: 69.9 kDa
• Gene Summary: Membrane-anchored forms may play a role in cellular adhesion.[UniProtKB/Swiss-Prot Function]