Parn (NM_028761) Mouse Tagged ORF Clone

CAT#: MR209527

Parn (Myc-DDK-tagged) - Mouse poly(A)-specific ribonuclease (deadenylation nuclease) (Parn)

Product Images

Circular map for MR209527

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Parn
• Synonyms: 1200003I18Rik; DAN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209527 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGATAATCCGGAGCAATTTTAAGATTAATCTTCACAAAGTGTACCAGGCCATAGAGGAGGCTGACT
  TCTTCGCCATCGATGGGGAGTTTTCAGGAATCAGCGATGGACCCTCAGTAACAGCATTAACAAGTGGTTT
  TGACACCCCAGAAGAGAGATATCAGAAGCTTAAAAAGCATTCCATGGACTTTTTGCTGTTTCAGTTTGGC
  CTTTGTGCTTTTAAGTATGACCACACAGATTCCAAGCATGTAACGAAGTCATTTAACTTCTATGTTTTCC
  CCAAGCCTTTCAGTAGGTCCTCACCAGATGTCAAGTTTGTTTGCCAGAGCTCCAGCATTGACTTCCTGGC
  GAGCCAAGGATTTGACTTTAATAAAGTGTTTTGCAGTGGGATTCCATATCTGAATCAGGAAGAAGAAAGG
  CAGCTGAGAGAGCAATTCGATGAAAAACGGTCTCAAGCCAATGGGGCAGGAGCTCTGGCAAAATGTCCTG
  TGACCATCCCTGAGGATCAGAAGAAGTTTATTGACCAAGTAATAGAGAAGATAGAGGATTTCCTACAAAG
  TGAAGAGAAGAGGAGCCTGGAACTTGACCCATGCACTGGGTTCCAAAGAAAACTTATTTATCAGACTTTG
  AGTTGGAAGTATCCTAAAGGCATTCATGTTGAGACATTAGAGACTGACAAGAAGGAAAGACATATAGTTA
  TCAGCAAGGTGGATGAAGAAGAACGCAAAAGGAGAGAGCAGGAGAAGTATACAAAGGAACAGGAGGAGCT
  GAATGATGCTGTGGGGTTTTCAAGAGTCATCCATGCCATTGCTAATTCGGGGAAGCTGGTTGTGGGACAC
  AACATGCTCTTGGATGTCATGCATACGATTCATCAGTTCTACTGCCCCCTGCCTGCGGACCTGAATGAGT
  TTAAGGAGATGGCAATATGTGTCTTCCCCAGACTCTTGGATACTAAGTTGATGGCCAGCACACAGCCTTT
  TAAGGATATCATTAACAACACATCCCTTGCAGAGTTGGAAAAGCGGTTGAAAGAGACACCTTTTGACCCT
  CCCAAAGTTGAAAGTGCAGAAGGCTTTCCAAGCTACGACACAGCTTCTGAGCAGCTTCATGAGGCGGGGT
  ACGATGCCTACATCACAGGGCTCTGCTTCATCTCCATGGCAAATTACTTAGGTTCTTTACTCAGTCCTCC
  AAAAATGTGTGTGTCTGCCAGATCAAAGCTCATTGAACCCTTTTTTAACAAGTTATTTCTTATGAGGGTC
  ATGGATATTCCCTATTTAAACTTGGAAGGGCCAGACTTGCAGCCTAAGCGGGACCATGTTCTCCACGTGA
  CCTTCCCCAAAGAGTGGAAAACCAGCGACCTGTACCAGCTCTTCAGCGCCTTCGGTAACATTCAGATATC
  CTGGATTGATGATACATCAGCCTTCGTTTCTCTCAGCCAGCCAGAACAAGTACAAATTGCCGTTAATACC
  AGCAAGTACGCTGAAAGTTATCGGATCCAGACCTATGCTGAGTATGTGGGAAAGAAGCAGGAAGGCAAGC
  AGGTCAAGAGGAAGTGGACAGAAGACAGTTGGAAGGAGGTGGACAGAAAGCGGCCCCACATGCAGGGCCC
  CTGTTACCACAGCAACAGCTTCACAGCAGCTGGCGTGCTTGGAAAGAGAACGCTGAGTCCTGACCCAAGG
  GAAGCTGCCTTGGAGGACAGAGAATCAGAGGAGGTATCTGACTCGGAGCTTGAACAGACAGATTCCTGTA
  CAGACCCCCTCCCAGAGGGAAGGAAAAAGTCCAAGAAGTTAAAACGAATGAAGAAGGAGCTTTCCTTGGC
  AGGAAGTGTCTCGGATAGCCCTGCCGTGCTCTTTGAAGTCCCTGACACATGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209527 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEIIRSNFKINLHKVYQAIEEADFFAIDGEFSGISDGPSVTALTSGFDTPEERYQKLKKHSMDFLLFQFG
  LCAFKYDHTDSKHVTKSFNFYVFPKPFSRSSPDVKFVCQSSSIDFLASQGFDFNKVFCSGIPYLNQEEER
  QLREQFDEKRSQANGAGALAKCPVTIPEDQKKFIDQVIEKIEDFLQSEEKRSLELDPCTGFQRKLIYQTL
  SWKYPKGIHVETLETDKKERHIVISKVDEEERKRREQEKYTKEQEELNDAVGFSRVIHAIANSGKLVVGH
  NMLLDVMHTIHQFYCPLPADLNEFKEMAICVFPRLLDTKLMASTQPFKDIINNTSLAELEKRLKETPFDP
  PKVESAEGFPSYDTASEQLHEAGYDAYITGLCFISMANYLGSLLSPPKMCVSARSKLIEPFFNKLFLMRV
  MDIPYLNLEGPDLQPKRDHVLHVTFPKEWKTSDLYQLFSAFGNIQISWIDDTSAFVSLSQPEQVQIAVNT
  SKYAESYRIQTYAEYVGKKQEGKQVKRKWTEDSWKEVDRKRPHMQGPCYHSNSFTAAGVLGKRTLSPDPR
  EAALEDRESEEVSDSELEQTDSCTDPLPEGRKKSKKLKRMKKELSLAGSVSDSPAVLFEVPDTW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028761
• ORF Size: 1875 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028761.1, NM_028761.2, NM_028761.3, NP_083037.1
• RefSeq Size: 2902 bp
• RefSeq ORF: 1875 bp
• Locus ID: 74108
• UniProt ID: Q8VDG3
• Cytogenetics: 16 A1
• MW: 71.6 kDa
• Gene Summary: 3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3' UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization (By similarity). Also able to recognize poly(A) tails of microRNAs such as MIR21 and H/ACA box snoRNAs (small nucleolar RNAs) leading to leading to microRNAs degradation or snoRNA increased stability (By similarity).[UniProtKB/Swiss-Prot Function]