Atg16l1 (BC049122) Mouse Tagged ORF Clone

CAT#: MR209513

Atg16l1 (Myc-DDK-tagged) - Mouse autophagy-related 16-like 1 (yeast) (cDNA clone MGC:61241 IMAGE:6813377)

Product Images

Circular map for MR209513

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Atg16l1
• Synonyms: WDR30, Atg16l
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209513 representing BC049122
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTCGGGCCTGCGCGCCGCAGACTTTCCCCGCTGGAAGCGTCACATCGCGGAGGAACTGAGGCGCC
  GGGACCGACTGCAGAGGCAGGCGTTCGAGGAGATCATTCTGCAGTATACCAAGTTGCTGGAAAAGTCAGA
  TCTTCATTCAGTATTGACCCAGAAACTACAAGCAGAAAAGCATGACATGCCAAATAGGCATGAAATAAGT
  CCTGGACATGATGGTGCGTGGAATGATAGTCAACTACAAGAAATGGCCCAGTTGAGGATCAAACACCAGG
  AAGAGCTGACCGAACTGCACAAGAAGCGTGGGGAGTTAGCTCAGTTGGTGATTGACCTGAACAACCAAAT
  GCAGCAGAAGGACAAGGAGATACAGATGAATGAAGCAAAGATTTCGGAGTATTTACAGACCATCTCTGAC
  CTGGAGACAAACTGCCTGGACCTGCGCACCAAACTGCAGGACCTCGAGGTAGCCAACCAGACCCTGAAGG
  ATGAGTATGACGCCCTGCAGATTACTTTTACTGCCCTAGAAGAGAAACTGAGGAAAACTACTGAGGAGAA
  CCAGGAACTGGTCACCAGATGGATGGCTGAGAAGGCCCAAGAAGCCAATCGCCTCAATGCAGAGAATGAG
  AAGGACTCCAGGAGGCGTCAAGCACGGCTGCAGAAGGAGCTTGCAGAAGCAGCAAAGGAACCTCTACCTG
  TTGAACAGGATGATGACATTGAAGTCATTGTGGATGAGACCTCAGACCACACAGAAGAGACCTCTCCCGT
  CCGAGCTGTCAGCAGAGCAGCTACTAAGCGACTCTCGCAGCCTGCTGGAGGCCTTCTGGATTCTATCACT
  AATATCTTTGGTCTGTCCGAATCTCCCCTTTTGGGACATCATTCTTCTGATGCTGCCAGGAGACGCTCTG
  TCTCTTCCATCCCAGTCCCCCAGGATATCATGGACACTCATCCTGCTTCTGGTAAAGATGTGAGAGTCCC
  AACTACTGCCTCGTATGTCTTCGATGCGCATGACGGAGAGGTCAACGCAGTGCAGTTCAGTCCAGGCTCC
  CGGTTGCTGGCCACTGGAGGCATGGACCGCAGGGTGAAACTTTGGGAAGCATTCGGAGATAAATGTGAAT
  TCAAGGGCTCCCTGTCTGGCAGTAATGCTGGAATTACAAGCATTGAATTTGATAGTGCTGGAGCTTACCT
  ATTAGCAGCTTCAAATGATTTTGCAAGCCGAATCTGGACTGTGGATGATTATCGATTACGGCACACACTC
  ACAGGCCACAGCGGGAAAGTCCTCTCTGCCAAGTTCCTGCTGGACAATGCACGGATTGTCTCAGGAAGTC
  ACGACCGGACCCTCAAACTCTGGGATCTCCGCAGCAAAGTCTGCATAAAAACAGTGTTTGCAGGATCCAG
  CTGCAATGACATTGTTTGCACTGAACAATGTGTAATGAGTGGACATTTTGACAAGAAAATTCGTTTCTGG
  GATATCCGGTCAGAGAGTGTGGTCCGAGAGATGGAACTGTTAGGGAAGATCACTGCTCTGGACCTAAACC
  CTGAGAGAACTGAGCTCCTGAGCTGCTCCCGTGATGACCTGCTAAAAGTCATCGACCTCCGGACAAATGC
  AGTCAAACAGACATTCAGTGCGCCTGGATTCAAATGCGGCTCTGACTGGACCCGGGTTGTCTTCAGCCCT
  GATGGCAGTTACGTGGCAGCAGGCTCAGCCGAGGGTTCTCTTTATGTCTGGAGTGTGCTGACAGGGAAAG
  TGGAGAAGGTTCTTTCAAAACAGCACAGCTCTTCTATCAATGCGGTGGCGTGGGCCCCCTCGGGCTTACA
  TGTTGTCAGTGTGGACAAAGGAAGCAGAGCTGTGCTGTGGGCACAGCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209513 representing BC049122
  Red=Cloning site Green=Tags(s)
  
  MSSGLRAADFPRWKRHIAEELRRRDRLQRQAFEEIILQYTKLLEKSDLHSVLTQKLQAEKHDMPNRHEIS
  PGHDGAWNDSQLQEMAQLRIKHQEELTELHKKRGELAQLVIDLNNQMQQKDKEIQMNEAKISEYLQTISD
  LETNCLDLRTKLQDLEVANQTLKDEYDALQITFTALEEKLRKTTEENQELVTRWMAEKAQEANRLNAENE
  KDSRRRQARLQKELAEAAKEPLPVEQDDDIEVIVDETSDHTEETSPVRAVSRAATKRLSQPAGGLLDSIT
  NIFGLSESPLLGHHSSDAARRRSVSSIPVPQDIMDTHPASGKDVRVPTTASYVFDAHDGEVNAVQFSPGS
  RLLATGGMDRRVKLWEAFGDKCEFKGSLSGSNAGITSIEFDSAGAYLLAASNDFASRIWTVDDYRLRHTL
  TGHSGKVLSAKFLLDNARIVSGSHDRTLKLWDLRSKVCIKTVFAGSSCNDIVCTEQCVMSGHFDKKIRFW
  DIRSESVVREMELLGKITALDLNPERTELLSCSRDDLLKVIDLRTNAVKQTFSAPGFKCGSDWTRVVFSP
  DGSYVAAGSAEGSLYVWSVLTGKVEKVLSKQHSSSINAVAWAPSGLHVVSVDKGSRAVLWAQP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC049122
• ORF Size: 1869 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC049122, AAH49122
• RefSeq Size: 3132 bp
• RefSeq ORF: 1871 bp
• Locus ID: 77040
• Cytogenetics: 1 D
• MW: 114.8 kDa
• Gene Summary: Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane (PubMed:18849966, PubMed:12665549, PubMed:24954904, PubMed:24553140, PubMed:23392225). Regulates mitochondrial antiviral signaling (MAVS)-dependent type I interferon (IFN-I) production (By similarity). Negatively regulates NOD1- and NOD2-driven inflammatory cytokine response (PubMed:24238340). Instead, promotes with NOD2 an autophagy-dependent antibacterial pathway. Plays a role in regulating morphology and function of Paneth cell.[UniProtKB/Swiss-Prot Function]