Rpap2 (NM_144911) Mouse Tagged ORF Clone

CAT#: MR209427

Rpap2 (Myc-DDK-tagged) - Mouse RNA polymerase II associated protein 2 (Rpap2), transcript variant 1

Product Images

Circular map for MR209427

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rpap2
• Synonyms: AW060207
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209427 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGACTCTGCCGTACCTTGCTCTCTAGGCCCAAGTACCCGGGCTTCCAGCACCCACCGAGATGCTA
  CCGGTACTAAACAGACCCGAGCCCTGAAACGAGGAGATGCATCTAAAAGGCAAGCAGAACTGGAAGCTGC
  TATACAAAGGAAGGTGGAGTTTGAAAGGAAAGCTGTGCGCATCGTAGAGCAGCTCTTAGAGGAGAACATC
  ACAGAAGAATTCCTGAAGGAGTGTGGCATGTTCATCACACCAGCTCACTACAGCGACGTTGTGGATGAGC
  GGTCCATCATCAAACTCTGTGGCTATCCTTTGTGTCAGAAGAAGCTAGGGGTTATCCCGAAACAGAAATA
  TCGAATTTCTACTAAAACCAATAAAGTCTATGATATTACTGAAAGGAAGTCTTTCTGCAGCAATTTTTGT
  TACAGAGCATCTAAATTTTTTGAAACACAGATTCCCAAAACTCCAGTATGGGTTCGAGAGGAAGAGAGGC
  CTCCTGACTTTCAGCTGCTGAAGAAAGGACAAAGCGGCAGTTCTGGAGAAGTAGTACAGTTCTTCCGAGA
  TGCCGTGACAGCAGCAGATGTTGACGGTTCCGGCGCCTTGGACGCGCAGTGTGACCCTGCTTCTTCCAGC
  TCTTGGAGTGAGCGAGCCAGTGATGAGGAGGAGCAAGGCTTTGTTTCTTCTCTTCTACCAGGAAACAGAC
  CAAAGGCAGTGGATACAAGACCGCAGCCGCACACAAAAAGCAGCATCATGAGAAAGAAGGCTGCTCAGAA
  CGTGGACTCCAAGGAGGGAGAGCAGACCGTGTCAGAGGTTACCGAGCAGCTGGATAATTGCAGATTAGAT
  AGTCAGGAGAAAGTGGCCACTTGTAAACGTCCTTTAAAGAAAGAAAGTACTCAGATTTCTTCACCTGGCC
  CTTTGTGTGACAGATTTAACACTTCAGCCATTTCTGAACATACACACGGTGTATCACAAGTTACGCTTGT
  AGGCATAAGCAAGAAGAGTGCTGAGCATTTCAGGAGCAAATTTGCCAAGTCAAATCCAGGTTCTGGGTCA
  GCCTCTGGTTTGGTACACGTGCGTCCTGAGGTTGCGAAGGCAAATTTACTCCGAGTTCTGTCGGACACTC
  TGACTGAATGGAAGACAGAAGAAACACTGAAGTTTTTATATGGCCAGAACCACGATTCTGTGTGTCTGAA
  ACCCTCTTCAGCCTCTGAGCCTGATGAAGAACTTGATGAAGATGACATAAGTTGTGACCCAGGTAGTTGT
  GGTCCTGCCCTGAGTCAAGCTCAGAACACCCTAGATGCAACACTGCCCTTTAGAGGCTCAGACACAGCCA
  TTAAGCCACTGCCAAGTTATGAGAGCTTGAAAAAGGAAACGGAGATGCTGAACCTGAGGGTCAGGGAGTT
  CTACAGAGGGCGCTGTGTTTTAAATGAAGACACTACCAAGTCTCAGGACTCTAAAGAGAGTGTGCTTCAG
  CGTGACCCCAGCTTCCCGCTGATAGATTCAAGTTCCCAGAACCAGATTAGAAGACGGATTGTTCTTGAAA
  AACTGAGCAAAGTATTGCCTGGACTTTTGGGTCCTCTTCAGATTACAATGGGAGATATTTACACTGAACT
  TAAAAACCTCATTCAGACTTTCAGATTATCAAATAGAAACATTATACACAAACCTGTGGAGTGGACTTTA
  ATTGCTGTGGTCTTGCTGTTACTATTGACACCGATTCTTGGCATTCAGAAGCATTCTCCGAAAAATGTGG
  TTTTTACACAGTTTATAGCCACCCTGCTGACAGAATTGCATTTAAAATTCGAAGATCTTGAAAAATTAAC
  CATGATATTTAGAACCAGCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209427 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MADSAVPCSLGPSTRASSTHRDATGTKQTRALKRGDASKRQAELEAAIQRKVEFERKAVRIVEQLLEENI
  TEEFLKECGMFITPAHYSDVVDERSIIKLCGYPLCQKKLGVIPKQKYRISTKTNKVYDITERKSFCSNFC
  YRASKFFETQIPKTPVWVREEERPPDFQLLKKGQSGSSGEVVQFFRDAVTAADVDGSGALDAQCDPASSS
  SWSERASDEEEQGFVSSLLPGNRPKAVDTRPQPHTKSSIMRKKAAQNVDSKEGEQTVSEVTEQLDNCRLD
  SQEKVATCKRPLKKESTQISSPGPLCDRFNTSAISEHTHGVSQVTLVGISKKSAEHFRSKFAKSNPGSGS
  ASGLVHVRPEVAKANLLRVLSDTLTEWKTEETLKFLYGQNHDSVCLKPSSASEPDEELDEDDISCDPGSC
  GPALSQAQNTLDATLPFRGSDTAIKPLPSYESLKKETEMLNLRVREFYRGRCVLNEDTTKSQDSKESVLQ
  RDPSFPLIDSSSQNQIRRRIVLEKLSKVLPGLLGPLQITMGDIYTELKNLIQTFRLSNRNIIHKPVEWTL
  IAVVLLLLLTPILGIQKHSPKNVVFTQFIATLLTELHLKFEDLEKLTMIFRTSC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144911
• ORF Size: 1845 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144911.1, NP_659160.1
• RefSeq Size: 2322 bp
• RefSeq ORF: 1845 bp
• Locus ID: 231571
• UniProt ID: Q8VC34
• Cytogenetics: 5 E5-F
• MW: 68.5 kDa
• Gene Summary: Protein phosphatase that displays CTD phosphatase activity and regulates transcription of snRNA genes. Recognizes and binds phosphorylated 'Ser-7' of the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and mediates dephosphorylation of 'Ser-5' of the CTD, thereby promoting transcription of snRNA genes.[UniProtKB/Swiss-Prot Function]