Lmnb1 (NM_010721) Mouse Tagged ORF Clone

CAT#: MR209179

Lmnb1 (Myc-DDK-tagged) - Mouse lamin B1 (Lmnb1)

Product Images

Circular map for MR209179

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lmnb1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209179 representing NM_010721
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACCGCGACCCCCGTGCAGCAGCAGCGGGCGGGCAGCCGCGCCAGCGCCCCCGCCACGCCGCTCA
  GCCCCACGCGCCTGTCGCGCCTGCAGGAGAAAGAGGAGCTGCGGGAGCTCAACGACCGCCTGGCTGTGTA
  CATCGATAAGGTGCGCAGCCTGGAGACGGAGAACAGCGCGCTGCAGCTGCAGGTGACCGAGCGCGAGGAG
  GTGCGCGGCCGCGAGCTCACCGGCCTCAAGGCTCTCTACGAGACCGAGCTGGCCGACGCACGCCGCGCTC
  TGGACGACACGGCCCGCGAGCGCGCCAAGCTCCAGATCGAGCTGGGCAAGTTCAAGGCCGAGCACGACCA
  GCTGCTGCTCAATTATGCCAAGAAGGAATCTGATCTCAGTGGAGCCCAGATCAAGCTTCGAGAGTATGAG
  GCGGCACTAAACTCTAAGGATGCGGCGCTGGCTACTGCCCTAGGGGACAAAAAGAGTTTAGAGGGAGACT
  TGGAGGATCTGAAAGATCAGATTGCCCAGCTAGAAGCATCCTTATCTGCCGCCAAAAAGCAGTTAGCAGA
  TGAAACTTTACTTAAAGTGGATTTGGAGAATCGCTGTCAGAGCCTTACTGAGGACTTGGAGTTTCGTAAA
  AATATGTATGAAGAGGAGATCAATGAGACAAGGAGGAAGCATGAGACCCGCTTGGTGGAAGTGGACTCTG
  GGCGTCAGATTGAGTATGAGTACAAGCTGGCTCAAGCCCTGCATGAGATGCGGGAGCAGCACGACGCGCA
  GGTGAGGCTGTACAAGGAAGAGCTGGAGCAGACCTACCACGCCAAGCTTGAGAATGCCAGACTCTCCTCA
  GAGATGAACACTTCCACTGTCAACAGTGCCCGGGAAGAGCTGATGGAGAGCCGGATGAGGATCGAGAGCC
  TCTCCTCACAGCTCTCTAACCTGCAGAAAGAGTCTAGAGCGTGTTTGGAAAGGATCCAGGAATTGGAGGA
  CATGCTTGCTAAGGAGAGAGACAACTCGCGCCGCATGCTGTCTGACAGAGAGAGAGAGATGGCGGAGATC
  AGGGACCAGATGCAGCAGCAGCTGAGTGATTATGAGCAGCTGCTGGACGTGAAGCTGGCCCTGGACATGG
  AGATCAGCGCCTACAGGAAGCTCCTGGAAGGCGAAGAAGAGCGGTTAAAGCTCTCTCCAAGCCCTTCTTC
  CCGGGTGACCGTGTCCAGAGCGTCCTCCAGTCGCAGTGTGCGCACCACCAGAGGAAAGCGGAAGAGAGTT
  GATGTGGAGGAGTCGGAGGCGAGCAGCAGTGTTAGCATTTCCCACTCTGCCTCAGCCACGGGGAACGTGT
  GCATTGAAGAGATAGATGTTGATGGGAAGTTTATTCGCTTGAAGAACACTTCTGAGCAGGATCAACCAAT
  GGGAGGCTGGGAGATGATCAGAAAAATTGGAGACACATCAGTCAGTTACAAATATACCTCAAGATATGTG
  CTGAAGGCTGGCCAGACTGTCACAGTGTGGGCTGCAAATGCTGGCGTCACAGCCAGCCCTCCAACTGACC
  TCATCTGGAAGAACCAGAACTCTTGGGGTACTGGTGAAGATGTGAAGGTTATATTGAAGAATTCTCAGGG
  AGAGGAGGTTGCTCAGAGAAGCACAGTCTTCAAGACCACCATACCCGAGGAGGAGGAGGAGGAGGAGGAG
  GAGCCCATCGGAGTGGCTGTGGAGGAGGAGCGTTTCCACCAGCAGGGAGCCCCAAGAGCATCCAATAAAA
  GCTGTGCCATTATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209179 representing NM_010721
  Red=Cloning site Green=Tags(s)
  
  MATATPVQQQRAGSRASAPATPLSPTRLSRLQEKEELRELNDRLAVYIDKVRSLETENSALQLQVTEREE
  VRGRELTGLKALYETELADARRALDDTARERAKLQIELGKFKAEHDQLLLNYAKKESDLSGAQIKLREYE
  AALNSKDAALATALGDKKSLEGDLEDLKDQIAQLEASLSAAKKQLADETLLKVDLENRCQSLTEDLEFRK
  NMYEEEINETRRKHETRLVEVDSGRQIEYEYKLAQALHEMREQHDAQVRLYKEELEQTYHAKLENARLSS
  EMNTSTVNSAREELMESRMRIESLSSQLSNLQKESRACLERIQELEDMLAKERDNSRRMLSDREREMAEI
  RDQMQQQLSDYEQLLDVKLALDMEISAYRKLLEGEEERLKLSPSPSSRVTVSRASSSRSVRTTRGKRKRV
  DVEESEASSSVSISHSASATGNVCIEEIDVDGKFIRLKNTSEQDQPMGGWEMIRKIGDTSVSYKYTSRYV
  LKAGQTVTVWAANAGVTASPPTDLIWKNQNSWGTGEDVKVILKNSQGEEVAQRSTVFKTTIPEEEEEEEE
  EPIGVAVEEERFHQQGAPRASNKSCAIM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010721
• ORF Size: 1764 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010721.2, NP_034851.2
• RefSeq Size: 2871 bp
• RefSeq ORF: 1767 bp
• Locus ID: 16906
• UniProt ID: P14733
• Cytogenetics: 18 30.84 cM
• MW: 66.8 kDa
• Gene Summary: Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.[UniProtKB/Swiss-Prot Function]