Ankrd13a (NM_026718) Mouse Tagged ORF Clone

CAT#: MR209168

Ankrd13a (Myc-DDK-tagged) - Mouse ankyrin repeat domain 13a (Ankrd13a)

Product Images

Circular map for MR209168

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ankrd13a
• Synonyms: 1100001D10Rik; ANKRD13; AU046136
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209168 representing NM_026718
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTCGGCCCGCGACACCAGCAGCCGCTTCCCGCTGCACCTCCTGGTCTGGAACAACGACTACGAGC
  AGCTGGAGAAGGAGCTGCGGGACCAGAATGCAGAGGCCTTGGACCCCCGGGGTCGGACATTACTGCACCT
  TGCAGTTTCTCTGGGGCATCTGGAATCTGCTCGAGTCTTGCTGCGCCATAAAGCAGATGTGACCAAAGAG
  AACGGCCAGGGATGGACAGTCTTACACGAAGCTGTGAGCACGGGCGATCCCGAGATGGTGTACACCGTCC
  TGCAGCACCGAGACTACCACAACACGTCCATGGCCCTGGAAGGTGTACCCGAGCTGCTACACAAAATCCT
  CGAGGCCCCGGATTTCTATGTGCAGATGAAGTGGGAGTTCACCAGCTGGGTGCCCTTGGTGTCTAGAATA
  TGCCCCAACGATGTCTGTCGCATATGGAAGAGTGGAGCCAAGCTGCGCGTGGACATCACCTTGCTGGGGT
  TTGAAAACATGAGCTGGATCAGAGGGAGGCGGAGCTTCATATTTAAGGGAGGAGACAACTGGGCGGAGCT
  GATGGAGGTCAACCACGATGACCGCGTGGTCACCACCGAACACTTCGATCTTTCCCAAGAAATGGAGCGC
  CTCACCCTGGACCTGATGAAGCCAAAAAGCAGGGAGGTGGAGAGGCGGCTGACAAGCCCAGTCATCAACA
  CCAGCCTGGATACTAAGAACGTTGCCTTTGAGAGAACTAAATCCGGATTCTGGGGCTGGAGGACAGATAA
  AGCTGAAGTTGTCAATGGTTACGAAGCCAAGGTTTACTCGGTAAACAACGTGAGCGTGATAACCAGGATC
  CGCACCGAGCACCTAACGGAGGAGGAGAAGAAGCGATACAAAGAGGACCGGAATCCACTGGAGTCTCTGC
  TCGGAACCGTGGAACATCAGTTTGGTGCTCAAGGGGACCTCGCTACGGAGTGTGCCACTGTGAACAACCC
  CACGGCCATCACTCCCGACGAGTACTTTGACGAAGACTTTGACCTGAAAGACAGGGACATTGGAAGACCA
  AAAGAGCTGACAATTAGAACGCAGAAGTTTAAGGCTACGCTGTGGATGTGCGAGGAGTTCCCACTCTCCC
  TCGTGGAGCAGGTCATCCCCATCATTGACCTCATGGCTCGCACCAGCGCTCACTTTGCCAGATTGAGAGA
  TTTCATCAAATTGGACTTCCCTCCTGGATTCCCTGTCAAAATAGAAATCCCCTTATTCCACGTCTTAAAC
  GCACGGATCACATTCGGAAATGTCAATGGCTGTAGCACTGCTGATGAGAGCCAGGGTGTGGAGGGGACCC
  CGGCCGAGGCAGTTTCCGAGGCCACAAACTTCGAGGTTGATCAGTCTGTCTTTGAAATCCCTGAATCCTA
  CCACATTCAAGACAATGGCAGAAACGTGCACCTGCAAGACGAAGACTATGAGATCATGCAGTTTGCCATC
  CAGCAGAGCCTGCTGGAGTCCAGTAGGAGCCAGGACCTTTCAGGACCAGCTTCCAACGGAGGGGTCAGCC
  ACACACACAGCTATGAAGCCCAGTATGAGAGGGCCATCCAGGAGAGTCTCCTAACCAACATGGAGGGCAG
  GTGCCCAGGTGGCCTCAGCGAGTCGAGCCGATTCGATAGCGACCTGCAGCTGGCCATGGAGCTGTCTGCC
  AAAGAGCTGGCGGAGCGGGAGCTCAGGCTCCAGGAGGAAGAAGCGGAGCTCCAGCAAGTCTTACAGCTGT
  CTCTCACTGAGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209168 representing NM_026718
  Red=Cloning site Green=Tags(s)
  
  MSSARDTSSRFPLHLLVWNNDYEQLEKELRDQNAEALDPRGRTLLHLAVSLGHLESARVLLRHKADVTKE
  NGQGWTVLHEAVSTGDPEMVYTVLQHRDYHNTSMALEGVPELLHKILEAPDFYVQMKWEFTSWVPLVSRI
  CPNDVCRIWKSGAKLRVDITLLGFENMSWIRGRRSFIFKGGDNWAELMEVNHDDRVVTTEHFDLSQEMER
  LTLDLMKPKSREVERRLTSPVINTSLDTKNVAFERTKSGFWGWRTDKAEVVNGYEAKVYSVNNVSVITRI
  RTEHLTEEEKKRYKEDRNPLESLLGTVEHQFGAQGDLATECATVNNPTAITPDEYFDEDFDLKDRDIGRP
  KELTIRTQKFKATLWMCEEFPLSLVEQVIPIIDLMARTSAHFARLRDFIKLDFPPGFPVKIEIPLFHVLN
  ARITFGNVNGCSTADESQGVEGTPAEAVSEATNFEVDQSVFEIPESYHIQDNGRNVHLQDEDYEIMQFAI
  QQSLLESSRSQDLSGPASNGGVSHTHSYEAQYERAIQESLLTNMEGRCPGGLSESSRFDSDLQLAMELSA
  KELAERELRLQEEEAELQQVLQLSLTEK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026718
• ORF Size: 1764 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026718.2, NP_080994.2
• RefSeq Size: 3316 bp
• RefSeq ORF: 1767 bp
• Locus ID: 68420
• UniProt ID: Q80UP5
• Cytogenetics: 5 F
• MW: 67.2 kDa
• Gene Summary: Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Does not bind 'Lys-48'-linked ubiquitin. Positively regulates the internalization of ligand-activated EGFR by binding to the Ub moiety of ubiquitinated EGFR at the cell membrane (By similarity).[UniProtKB/Swiss-Prot Function]