Gckr (NM_144909) Mouse Tagged ORF Clone

CAT#: MR209160

Gckr (Myc-DDK-tagged) - Mouse glucokinase regulatory protein (Gckr)

Product Images

Circular map for MR209160

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gckr
• Synonyms: GKRP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209160 representing NM_144909
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAAGCACCAAGCGGTATCAGCATGTGATCGAGACCCCTGAGCCTGGGGAATGGGAGTTGTCAGGGT
  ATGAAGCAGCTGTGCCAATCACAGAGAAGTCCAACCCACTGACCCGGAACTTGGACAAAGCAGATGCAGA
  GAAAATTGTTCAACTGCTGGGGCAGTGTGATGCTGAGATATTCCAGGAGGAGGGGCAAATCATGCCCACC
  TACCAGCGACTGTACAGTGAGTCAGTTCTGACCACCATGTTGCAAGTGGCTGGCAAGGTCCAGGAAGTGC
  TGAAGGAGCCAGATGGGGGCCTGGTGGTGCTGAGTGGAGGGGGCACCTCTGGTCGTATGGCATTCCTTAT
  GTCTGTGTCTTTCAACCAGCTGATGAAAGGTCTGGGACAAAAACCTCTTTACACATACCTCATTGCAGGG
  GGTGACAGGTCTGTTGTAGCCTCTCGGGAACGGACAGAAGATAGCGCCCTACACGGAATCGAGGAGCTGA
  AGAAGGTGGCTGCTGGGAAAAAGAGAGTGGTCGTTATAGGCATTTCCGTGGGACTCTCTGCGCCCTTTGT
  GGCAGGCCAGATGGACTACTGCATGGATAACACAGCTGTCTTCTTGCCGGTCCTGGTTGGCTTCAATCCG
  GTGAGCATGGCCAGAAATGATCCCATTGAAGACTGGAGATCGACATTCCGACAAGTGGCAGAGCGGATGC
  AGAAGATGCAGGAGAAACAGGAAGCCTTTGTGCTCAATCCTGCCATCGGGCCTGAGGGGCTCAGTGGCTC
  TTCCCGAATGAAAGGTGGAAGCGCCACCAAGATTCTACTGGAAACCCTGCTACTAGCAGCCCATAAGACT
  GTGGACCAGGGTGTTGTGTCCTCTCAAAGATGCCTTCTGGAAATCCTGAGGACATTTGAGCGGGCTCATC
  AGGTAACCTACAGTCAAAGTTCCAAAATTGCCACTCTGACGAAGCAAGTTGGCATCAGCCTGGAGAAAAA
  AGGCCACGTGCACTTGGTTGGCTGGCAGACCCTCGGTATCATCGCCATTATGGATGGGGTAGAGTGTATC
  CACACTTTTGGTGCTGATTTCCGAGATATCCGTGGCTTTCTTATTGGTGACCACAATGACATGTTTAACC
  AGAAGGATGAGCTCAGCAATCAGGGTCCCCAGTTCACCTTCTCTCAGGATGACTTCCTGACTTCTGTTCT
  GCCATCCCTTACGGAAATTGACACTGTGGTCTTCATTTTTACCCTGGATGATAACCTCGCAGAAGTACAG
  GCCCTGGCAGAAAGGGTGAGGGAGAAGAGTTGGAACATCCAGGCCCTGGTGCACAGCACAGTGGGGCAGT
  CCTTGCCAGCTCCTCTAAAGAAGCTCTTTCCCTCGCTCATCAGCATCACATGGCCACTTCTTTTCTTCGA
  TTATGAAGGGAGCTACGTTCAGAAGTTCCAGCGTGAGTTAAGCACCAAGTGGGTGTTGAATACAAGGTTC
  TCAGGACAGTCCAAGGCTCGCTGCATTGAGAGTCTTCTTCAAGTGATACATTTCCCTCAACCGCTGTCGA
  ATGATGTCCGCGCGGCCCCCATCTCCTGCCATGTCCAGGTTGCCCACGAGAAGGAAAAGGTGATCCCCAC
  AGCCTTGCTGAGTCTCCTACTCAGGTGCTCCATCACTGAGGCTAAGGAACGCCTGGCTGCAGCTTCTTCA
  GTCTGTGAGGTTGTTAGGAGCGCCCTCTCTGGGCCAGGTCAGAAACGCAGCATCCAAGCCTTTGGAGACC
  CTGTGGTGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209160 representing NM_144909
  Red=Cloning site Green=Tags(s)
  
  MPSTKRYQHVIETPEPGEWELSGYEAAVPITEKSNPLTRNLDKADAEKIVQLLGQCDAEIFQEEGQIMPT
  YQRLYSESVLTTMLQVAGKVQEVLKEPDGGLVVLSGGGTSGRMAFLMSVSFNQLMKGLGQKPLYTYLIAG
  GDRSVVASRERTEDSALHGIEELKKVAAGKKRVVVIGISVGLSAPFVAGQMDYCMDNTAVFLPVLVGFNP
  VSMARNDPIEDWRSTFRQVAERMQKMQEKQEAFVLNPAIGPEGLSGSSRMKGGSATKILLETLLLAAHKT
  VDQGVVSSQRCLLEILRTFERAHQVTYSQSSKIATLTKQVGISLEKKGHVHLVGWQTLGIIAIMDGVECI
  HTFGADFRDIRGFLIGDHNDMFNQKDELSNQGPQFTFSQDDFLTSVLPSLTEIDTVVFIFTLDDNLAEVQ
  ALAERVREKSWNIQALVHSTVGQSLPAPLKKLFPSLISITWPLLFFDYEGSYVQKFQRELSTKWVLNTRF
  SGQSKARCIESLLQVIHFPQPLSNDVRAAPISCHVQVAHEKEKVIPTALLSLLLRCSITEAKERLAAASS
  VCEVVRSALSGPGQKRSIQAFGDPVVP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144909
• ORF Size: 1761 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144909.2
• RefSeq Size: 2024 bp
• RefSeq ORF: 1764 bp
• Locus ID: 231103
• UniProt ID: Q91X44
• Cytogenetics: 5 17.27 cM
• MW: 65.1 kDa
• Gene Summary: Inhibits glucokinase (GCK) by forming an inactive complex with this enzyme. The affinity of GCKR for GCK is modulated by fructose metabolites: GCKR with bound fructose 6-phosphate has increased affinity for GCK, while GCKR with bound fructose 1-phosphate has strongly decreased affinity for GCK and does not inhibit GCK activity (By similarity).[UniProtKB/Swiss-Prot Function]