Acsm1 (NM_054094) Mouse Tagged ORF Clone

CAT#: MR209014

Acsm1 (Myc-DDK-tagged) - Mouse acyl-CoA synthetase medium-chain family member 1 (Acsm1)

Product Images

Circular map for MR209014

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Acsm1
• Synonyms: Acas3; Bucs1; Macs
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209014 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGTGGCTGAAGAGTTTCCAGATCTGCAAAGTCTTACAGGGCTTCTCCCTAAGCCCCACCCAGCTGC
  ACAGACGGTTGTTTTCAAGAGTTGGAGCTCCAAGATGGAATGACCATGATTCACCTGAGGAGTTTAACTT
  TGCAAGTGATGTCCTGGACTACTGGGCTCAAATGGAGGAGGAGGGCAAGAGAGGACCAAGTCCAGCCTTT
  TGGTGGGTGAATGGCCAAGGAGATGAAATAAAGTGGAGCTTCAGGAAGCTGAGGGACCTCACCTGTCGCA
  CTGCCAACGTCTTTGAGCAGATTTGTGGCCTGCAGCAAGGAGATCACCTGGCCTTGATTCTGCCCCGAGT
  GCCCGAGTGGTGGTTGGTGACAGTGGGCTGCATGCGAACAGGGATCATCTTCATGCCTGGGACTACCCAA
  CTGAAAGCCAAGGACATTCTCTACCGAATACAAATATCTCGAGCCAAAGCCATTGTGACCACAGCTAGCC
  TTGTCCCAGAGGTGGAATCTGTGGCTTCTGAGTGTCCTGATCTGAAAACCAAGCTGGTGGTGTCTGATCA
  CAGCCATGAAGGGTGGCTTGATTTCTGTTCACTGATTAAATCAGCATCCCCAGACCATACTTGTATTAAG
  TCAAAGATGAAGGATCCCATGGCCATCTTCTTCACCAGTGGGACCACAGGCTACCCCAAGATGGCAAAGC
  ACAACCAGGGACTTGCCTTCCGGTCATATATCCCTTCATGCAGAAAATTATTGAAGCTGAAGACATCTGA
  CATCTTGTGGTGCATGTCAGACCCAGGATGGATTCTGGCTACCGTGGGGTGCCTGATCGAGCCATGGACA
  TCAGGATGTACAGTCTTCATCCACCACCTCCCTCAATTCGACCCCAAAGTCATTGTAGAGGTACTGTTCA
  AATACCCCATCACTCAGTGCCTTGCTGCCCCAGGCGTGTATCGAATGGTTCTTCAGCAGAAAACCTCCAA
  CCTCAGGTTCCCCACCCTTGAGCATTGCACTACTGGTGGGGAGAGCCTGCTGCCTGAGGAGTATGAGCAG
  TGGAAGCAAAGGACAGGTCTTTCCATCCACGAGGTCTATGGACAGTCAGAAACGGGGATCAGCAGTGCCA
  CCCTCCGGGAAATGAAGATCAAGCGAGGCTCCATAGGGAAGGCCATCTTACCCTTTGACTTGCAGATCAT
  CGATGAAAAGGGCAACATCCTCCCACCCAACACTGAAGGATACATTGGCATCAGGATCAAGCCCACCAGG
  CCTCTAGGCCTCTTCATGGAATATGAGAATAGCCCAGAGAGCACATCTGAAGTGGAGTGTGGGGACTTTT
  ACAATAGTGGGGATAGAGCGACCATTGATGAAGAGGGCTACATCTGGTTCTTGGGAAGGGGCGATGATGT
  CATCAATGCTTCCGGGTATCGCATCGGGCCTGTAGAGGTGGAGAACGCCTTGGCGGAGCACCCAGCAGTG
  GCAGAGTCTGCGGTGGTGAGCAGCCCGGACAAGGATCGAGGAGAGGTGGTGAAGGCGTTTATTGTCCTCA
  ACCCAGAGTTCCTGTCACACGATCAGGAACAGCTTATCAAAGAGCTACAGCATCATGTGAAGTCAGTGAC
  GGCACCATACAAGTACCCCAGGAAGGTGGAGTTTGTTTCAGAATTGCCCAAAACTGTCACAGGCAAAATC
  AAAAGGAAGGAACTTCGAAACAAGGAGTTTGGTCAGCTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209014 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQWLKSFQICKVLQGFSLSPTQLHRRLFSRVGAPRWNDHDSPEEFNFASDVLDYWAQMEEEGKRGPSPAF
  WWVNGQGDEIKWSFRKLRDLTCRTANVFEQICGLQQGDHLALILPRVPEWWLVTVGCMRTGIIFMPGTTQ
  LKAKDILYRIQISRAKAIVTTASLVPEVESVASECPDLKTKLVVSDHSHEGWLDFCSLIKSASPDHTCIK
  SKMKDPMAIFFTSGTTGYPKMAKHNQGLAFRSYIPSCRKLLKLKTSDILWCMSDPGWILATVGCLIEPWT
  SGCTVFIHHLPQFDPKVIVEVLFKYPITQCLAAPGVYRMVLQQKTSNLRFPTLEHCTTGGESLLPEEYEQ
  WKQRTGLSIHEVYGQSETGISSATLREMKIKRGSIGKAILPFDLQIIDEKGNILPPNTEGYIGIRIKPTR
  PLGLFMEYENSPESTSEVECGDFYNSGDRATIDEEGYIWFLGRGDDVINASGYRIGPVEVENALAEHPAV
  AESAVVSSPDKDRGEVVKAFIVLNPEFLSHDQEQLIKELQHHVKSVTAPYKYPRKVEFVSELPKTVTGKI
  KRKELRNKEFGQL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_054094
• ORF Size: 1722 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_054094.6
• RefSeq Size: 2041 bp
• RefSeq ORF: 1722 bp
• Locus ID: 117147
• UniProt ID: Q91VA0
• Cytogenetics: 7 F2
• MW: 64.8 kDa
• Gene Summary: Functions as GTP-dependent lipoate-activating enzyme that generates the substrate for lipoyltransferase (By similarity). Has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). Acts on acids from C(4) to C(11) and on the corresponding 3-hydroxy- and 2,3- or 3,4-unsaturated acids (in vitro).[UniProtKB/Swiss-Prot Function]