Parp2 (NM_009632) Mouse Tagged ORF Clone

CAT#: MR208862

Parp2 (Myc-DDK-tagged) - Mouse poly (ADP-ribose) polymerase family, member 2 (Parp2)

Product Images

Circular map for MR208862

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Parp2
• Synonyms: Adprt2; Adprtl2; ARTD2; Aspartl2; C78626; PARP-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208862 representing NM_009632
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCGCGGCGGCAGAGATCAGGCTCTGGAAGGCGAGTGCTAAATGAAGCCAAGAAAGTTGATAATG
  GCAACAAAGCAACAGAAGACGACTCTCCTCCTGGCAAGAAGATGCGCACGTGCCAGAGAAAAGGGCCTAT
  GGCTGGAGGGAAGGACGCAGACAGGACAAAAGACAATCGAGACTCTGTGAAGACCTTGCTGTTAAAGGGC
  AAAGCCCCTGTGGACCCAGAGTGTGCAGCCAAGCTGGGAAAGGCTCATGTGTATTGTGAAGGAGATGATG
  TCTATGATGTCATGCTAAATCAAACCAATCTCCAGTTCAACAACAACAAGTACTACCTTATTCAGCTGTT
  AGAAGATGATGCCCAGAGGAACTTCAGTGTTTGGATGAGGTGGGGCCGAGTTGGAAAGACGGGGCAGCAC
  AGCTTGGTGACTTGTTCTGGTGACCTCAACAAAGCAAAAGAAATATTTCAGAAAAAATTCCTTGACAAAA
  CTAAAAACAATTGGGAGGACCGTGAGAACTTTGAAAAAGTACCTGGAAAATACGACATGTTACAGATGGA
  CTATGCTGCCAGCACGCAGGATGAAAGTAAAACAAAAGAAGAGGAAACTTTGAAGCCTGAGTCTCAGCTG
  GATCTTCGAGTCCAGGAGCTGCTAAAGTTGATCTGTAACGTGCAGACCATGGAAGAAATGATGATTGAGA
  TGAAGTATGACACCAAGAGAGCCCCGCTTGGAAAGCTGACAGTGGCGCAAATCAAGGCCGGTTACCAGTC
  TCTCAAGAAGATTGAGGACTGCATCCGCGCTGGCCAGCATGGGCGAGCGCTTGTTGAAGCGTGCAATGAA
  TTCTACACCAGGATCCCTCATGACTTTGGACTCTCCATCCCTCCAGTAATCCGGACAGAGAAGGAACTGT
  CAGACAAAGTAAAACTGCTAGAGGCATTGGGAGACATTGAAATTGCCCTTAAACTGGTGAAGTCAGAGCG
  CCAAGGCCTAGAACACCCACTGGACCAACACTATAGAAACCTACACTGTGCTTTGCGTCCTCTGGACCAT
  GAAAGTAATGAGTTTAAGGTGATTTCTCAGTACCTACAGTCTACGCATGCTCCTACACACAAGGACTATA
  CTATGACCTTGCTGGATGTTTTCGAAGTAGAGAAGGAAGGGGAGAAAGAGGCCTTCAGGGAGGACCTTCC
  TAACAGGATGCTGCTCTGGCATGGATCCAGGCTGAGTAACTGGGTGGGGATCCTGAGCCACGGGCTTAGA
  GTTGCCCCACCTGAGGCTCCCATCACAGGTTATATGTTTGGAAAAGGAATCTACTTTGCTGACATGTCCT
  CCAAGAGTGCCAATTACTGCTTTGCCTCTCGCCTAAAGAATACAGGATTGCTTCTTCTGTCAGAGGTAGC
  TCTAGGTCAGTGTAATGAACTACTGGAGGCCAATCCTAAAGCACAAGGATTGCTTCGGGGCAAGCATAGC
  ACCAAGGGGATGGGAAAGATGGCTCCCAGCCCTGCCCACTTCATCACCCTGAATGGGAGTACAGTGCCCT
  TAGGACCAGCAAGTGACACAGGAATTCTCAATCCAGAGGGGTACACCCTCAACTACAATGAGTTTATTGT
  TTATAGCCCCAACCAGGTCCGTATGCGATACCTTCTAAAGATTCAATTTAACTTCCTGCAGCTATGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208862 representing NM_009632
  Red=Cloning site Green=Tags(s)
  
  MAPRRQRSGSGRRVLNEAKKVDNGNKATEDDSPPGKKMRTCQRKGPMAGGKDADRTKDNRDSVKTLLLKG
  KAPVDPECAAKLGKAHVYCEGDDVYDVMLNQTNLQFNNNKYYLIQLLEDDAQRNFSVWMRWGRVGKTGQH
  SLVTCSGDLNKAKEIFQKKFLDKTKNNWEDRENFEKVPGKYDMLQMDYAASTQDESKTKEEETLKPESQL
  DLRVQELLKLICNVQTMEEMMIEMKYDTKRAPLGKLTVAQIKAGYQSLKKIEDCIRAGQHGRALVEACNE
  FYTRIPHDFGLSIPPVIRTEKELSDKVKLLEALGDIEIALKLVKSERQGLEHPLDQHYRNLHCALRPLDH
  ESNEFKVISQYLQSTHAPTHKDYTMTLLDVFEVEKEGEKEAFREDLPNRMLLWHGSRLSNWVGILSHGLR
  VAPPEAPITGYMFGKGIYFADMSSKSANYCFASRLKNTGLLLLSEVALGQCNELLEANPKAQGLLRGKHS
  TKGMGKMAPSPAHFITLNGSTVPLGPASDTGILNPEGYTLNYNEFIVYSPNQVRMRYLLKIQFNFLQLW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009632
• ORF Size: 1677 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009632.3
• RefSeq Size: 1834 bp
• RefSeq ORF: 1680 bp
• Locus ID: 11546
• UniProt ID: O88554
• Cytogenetics: 14 C1
• MW: 63.8 kDa
• Gene Summary: Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair (PubMed:10364231). Mainly mediates glutamate and aspartate ADP-ribosylation of target proteins: the ADP-D-ribosyl group of NAD(+) is transferred to the acceptor carboxyl group of glutamate and aspartate residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units (By similarity). ADP-ribosylation follows DNA damage and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks (PubMed:10364231). Also mediates serine ADP-ribosylation of target proteins following interaction with HPF1; HPF1 conferring serine specificity (By similarity). In addition to proteins, also able to ADP-ribosylate DNA: preferentially acts on 5'-terminal phosphates at DNA strand breaks termini in nicked duplex (By similarity).[UniProtKB/Swiss-Prot Function]