Isyna1 (NM_023627) Mouse Tagged ORF Clone

CAT#: MR208826

Isyna1 (Myc-DDK-tagged) - Mouse myo-inositol 1-phosphate synthase A1 (Isyna1)

Product Images

Circular map for MR208826

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Isyna1
• Synonyms: 1300017C10Rik; AU018670
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208826 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCGCCGCCGAGATCCTCGTGGATAGCCCGGACGTGGTCTACAGCCCCGAAACCATCGAGGCGC
  GCTACGAGTACCGGACAACGCGCGTCAGCCGCGAGGGCGGCGTGCTGCGGGTGCAGCCCAGGGCTACGCG
  TTTCACCTTCCGCACCGCCCGGCAGGTGCCCCGACTCGGGGTCATGTTGGTCGGCTGGGGCGGGAACAAC
  GGCTCCACGCTCACTGCTGCTGTTCTGGCCAATCGGCTGCGCCTAACCTGGCCCACGCGCACAGGTCGCA
  AGGAGGCAAACTATTATGGATCGTTGACCCAGGCGGGCACCGTGAACTTGGGTTTGGATGAGAACGGCCG
  GGAGGTGTTTGTGCCCTTCAGTGCGCTGCTACCCATGGTGGCCCCCAACGACCTGGTGTTTGATGGCTGG
  GATATCTCGTCGCTGAACCTGGCCGAGGCCATGCGGCGCGCGCAGGTCCTGGACTGCGGTCTGCAGGAAC
  AGCTGTGGCCCCACATGGAGAGCCTGCGTCCGCGGCCCTCAGTCTACATTCCTGAGTTCATCGCTGCCAA
  CCAGACAGCACGTGCGGACAACCTCATCCCTGGCACACGTGCCCAACAGTTGGAGCAAATCCGAAAGGAC
  ATTAGAGATTTCCGATCCAGTGCGGGATTGGATAAGGTCATCGTGCTGTGGACCGCCAATACGGAGCGCT
  TCTGCGAGGTGGTCCCAGGTCGCAATGACACAGCTGAAAACTTGCTACATACTATCCAGCTTGGCCTGGA
  GGTGTCACCGTCCACACTTTTTGCTGTGGCCAGCATCCTGGAGGACTGCGCCTTCCTCAATGGATCCCCA
  CAGAACACACTGGTACCCGGTGCCCTGGAACTGGCTTCGCAGCGCCATGTGTTTGTAGGTGGTGATGACT
  TCAAGTCAGGGCAGACTAAGGTCAAGTCTGTCCTGGTGGACTTCCTCATCGGCTCTGGGCTCAAGACCAT
  GTCCATCGTGAGCTATAACCACCTGGGCAACAACGACGGGCAGAACCTGTCTGCACCGCTGCAGTTCCGC
  TCCAAGGAGGTGACAAAGAGCAGTGTGGTGGACGACATGGTTCACAGCAACCATGTGCTCTACGCGCCTG
  GAGAGCGGCCAGACCACTGTGTGGTGATCAAATATGTGCCCTATGTTGGCGACAGCAAGCGTGCCTTGGA
  CGAGTACACCTCCGAGCTGATGCTGGGTGGGACAAACACCTTGGTGCTCCATAATACCTGCGAGGACTCG
  CTCCTGGCCGCGCCCATCATGCTGGACCTAGTGCTGCTCACAGAGCTGTGTCAGCGCGTGAGCTTCTGCA
  CAGACTCGGACCCCGAGCCTCAGGGCTTCCACACAGTGCTGTCCCTGCTTAGCTTCCTGTTTAAGGCCCC
  GCTTGTGCCCCCGGGCAGCCCTGTGGTGAATGCCCTCTTCCGCCAGCGCAGCTGTATCGAGAATATTTTC
  AGGGCTTGCGTGGGGCTCCCGCCCCAGAACCACATGCTATTAGAGCACAAGATGGAGCGTCCAGGCCCAG
  GCATCAAGCCAGGAGAGGTGGTGGCCACCAGCCCACTGCCTTGCAAGAAAGAGCCCACACCTGCCACCAA
  TGGCTGCACCGGCGATGCCAATGGGCACCCGCAGGCACCAACACCAAAGCTGTCCACTGCT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208826 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEPAAEILVDSPDVVYSPETIEARYEYRTTRVSREGGVLRVQPRATRFTFRTARQVPRLGVMLVGWGGNN
  GSTLTAAVLANRLRLTWPTRTGRKEANYYGSLTQAGTVNLGLDENGREVFVPFSALLPMVAPNDLVFDGW
  DISSLNLAEAMRRAQVLDCGLQEQLWPHMESLRPRPSVYIPEFIAANQTARADNLIPGTRAQQLEQIRKD
  IRDFRSSAGLDKVIVLWTANTERFCEVVPGRNDTAENLLHTIQLGLEVSPSTLFAVASILEDCAFLNGSP
  QNTLVPGALELASQRHVFVGGDDFKSGQTKVKSVLVDFLIGSGLKTMSIVSYNHLGNNDGQNLSAPLQFR
  SKEVTKSSVVDDMVHSNHVLYAPGERPDHCVVIKYVPYVGDSKRALDEYTSELMLGGTNTLVLHNTCEDS
  LLAAPIMLDLVLLTELCQRVSFCTDSDPEPQGFHTVLSLLSFLFKAPLVPPGSPVVNALFRQRSCIENIF
  RACVGLPPQNHMLLEHKMERPGPGIKPGEVVATSPLPCKKEPTPATNGCTGDANGHPQAPTPKLSTA
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023627
• ORF Size: 1674 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023627.1, NP_076116.1
• RefSeq Size: 1850 bp
• RefSeq ORF: 1674 bp
• Locus ID: 71780
• UniProt ID: Q9JHU9
• Cytogenetics: 8 B3.3
• MW: 60.9 kDa
• Gene Summary: Key enzyme in myo-inositol biosynthesis pathway that catalyzes the conversion of glucose 6-phosphate to 1-myo-inositol 1-phosphate in a NAD-dependent manner. Rate-limiting enzyme in the synthesis of all inositol-containing compounds (By similarity).[UniProtKB/Swiss-Prot Function]