D2hgdh (NM_178882) Mouse Tagged ORF Clone

CAT#: MR208571

D2hgdh (Myc-DDK-tagged) - Mouse D-2-hydroxyglutarate dehydrogenase (D2hgdh), nuclear gene encoding mitochondrial protein

Product Images

Circular map for MR208571

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: D2hgdh
• Synonyms: AA408776; AA408778; AI325464
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208571 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCTCCCTCTGGTGTCCAGATGGTCTGCAAGGGTCCTCTGGGCATCTCCAGGATGGAGGAGGACCT
  ACACCCAGAGAGCTTGTGTGGGGTTGAAGCGGCTGGGTTGCCCTCGGGGTGTGTACAGCCCCTTGGCTCA
  CAGAGCGTACTCCGTGGTAGCTGGCAGCCCAGAGGTGACGCTGACCCCTGAGCGATATCCGGTGCAACGG
  CTGCCGTTTTCTACTGTGTCCGAGGAGGACCTGGCTGCATTTGAATGCATCATACCTGGCAGAGTTATCA
  CAGATCCAGAGCAACTGCAGACATGCAACGTTGACTGGCTGAAGACCGTCCGGGGTTGTAGTAAGGTGTT
  GCTGAGACCCCAGACCTCAGAGGAAGTCTCCCAGATTCTTAGGCACTGCTACAAGAGGAACTTGGCTGTG
  AACCCACAGGGCGGGAACACTGGCATGGTGGGTGGCAGTGTCCCTGTCTTTGACGAAGTCATCCTGTCCA
  CTGCCCTCATGAATCAAGTCATCAGCTTTCACGATGTATCTGGAATCCTGGTTTGCCAGGCAGGCTGTGT
  GCTGGAGGAGCTGAGCAGGTACGTGCAGGAGCGGGACTTCATCATGCCACTGGACTTGGGAGCGAAGGGC
  AGCTGCCACATCGGGGGAAATGTGGCAACAAATGCTGGTGGCCTGCGATTCCTGAGATATGGCTCCCTTC
  GAGGGACTGTCCTGGGCCTGGAAGTGGTACTCGCTGATGGGACCATCCTGAACTGCCTGACATCTCTGAG
  GAAGGACAACACAGGCTATGACCTGAAGCAGATGTTCATTGGGTCAGAGGGCACCCTGGGAGTCATCACT
  GCTGTGTCCATTGTGTGCCCACCCAGGCCTAAGGCTGTGAATGTGGCTTTCCTTGGCTGCCCTGGGTTCG
  CTGAGGTTCTGCAGACCTTCCGCACCTGCAGGGGGATGCTGGGTGAGATCCTGTCTGCCTTTGAGTTCAT
  GGATACTGAATGCATGCAGTTGGTTGGACAGCACCTCCAGCTGACCAACCCAGTACAAGAGAGTCCATTC
  TATGTCCTGGTTGAGACCTCAGGCTCCAGTGCAGGACATGATGCTGAGAAGCTGACGAACGTCCTGGAGC
  AGGTGCTGAATTCTGGCCTGGTGACAGATGGCACTATGGCCACCGACCAGAGGAAAGTCCAGATGCTGTG
  GGCCTTGCGGGAGAGGATCACAGAGGCACTCAGCCGCGATGGCTATGTGTTCAAGTATGACTTATCTCTC
  CCTGTGGAGCGGCTCTACGACCTTGTGATCGACCTACGCACCCGCCTTGGCCCCCGTGCCAAGCACGTGG
  TGGGATATGGACACCTGGGGGATGGCAACCTTCACCTGAACGTGACAGCAGAGGCCTTTAGCCGGGAGTT
  GCTCGGTGCCTTGGAACCTTATGTGTATGCCTGGACAGCTGAGCAGCGGGGCAGTGTCAGTGCGGAGCAC
  GGCCTGGGCTTCAAGAAGAAGGATGTACTTGGCTATAGCAAGCCACCTGTGGCCGTGACGCTTATGCAAC
  AGCTCAAGGCCATGCTGGACCCCAAAGGCATCCTGAACCCCTATAAGACTCTACCTGCCCAGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208571 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVLPLVSRWSARVLWASPGWRRTYTQRACVGLKRLGCPRGVYSPLAHRAYSVVAGSPEVTLTPERYPVQR
  LPFSTVSEEDLAAFECIIPGRVITDPEQLQTCNVDWLKTVRGCSKVLLRPQTSEEVSQILRHCYKRNLAV
  NPQGGNTGMVGGSVPVFDEVILSTALMNQVISFHDVSGILVCQAGCVLEELSRYVQERDFIMPLDLGAKG
  SCHIGGNVATNAGGLRFLRYGSLRGTVLGLEVVLADGTILNCLTSLRKDNTGYDLKQMFIGSEGTLGVIT
  AVSIVCPPRPKAVNVAFLGCPGFAEVLQTFRTCRGMLGEILSAFEFMDTECMQLVGQHLQLTNPVQESPF
  YVLVETSGSSAGHDAEKLTNVLEQVLNSGLVTDGTMATDQRKVQMLWALRERITEALSRDGYVFKYDLSL
  PVERLYDLVIDLRTRLGPRAKHVVGYGHLGDGNLHLNVTAEAFSRELLGALEPYVYAWTAEQRGSVSAEH
  GLGFKKKDVLGYSKPPVAVTLMQQLKAMLDPKGILNPYKTLPAQA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178882
• ORF Size: 1608 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178882.1, NM_178882.2, NM_178882.3, NM_178882.4, NP_849213.1
• RefSeq Size: 3058 bp
• RefSeq ORF: 1608 bp
• Locus ID: 98314
• UniProt ID: Q8CIM3
• Cytogenetics: 1 D
• MW: 58.6 kDa
• Gene Summary: Catalyzes the oxidation of D-2-hydroxyglutarate to alpha-ketoglutarate.[UniProtKB/Swiss-Prot Function]