Slc25a25 (NM_146118) Mouse Tagged ORF Clone

CAT#: MR208251

Slc25a25 (Myc-DDK-tagged) - Mouse solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 25 (Slc25a25), nuclear gene encoding mitochondrial protein, transcript variant 1

Product Images

Circular map for MR208251

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc25a25
• Synonyms: 1110030N17Rik; MCSC; mKIAA1896
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208251 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGAGCAGTGTGTTGTGCCGCTGCGTGGCCTCCCCGCCGCCGGACGCCGCCGCCACCGCCTCGTCGT
  CCGCCTCGTCGCCGGCTTCTGTAGGGGACCCGTGCGGCGGCGCCGTCTGTGGCGGCCCGGACCACCAGCT
  ACGCCTGTGGAGCCTCTTCCAGACACTCGACGTCAACCGCGACGGCGGCCTGTGTGTCAACGACCTGGCG
  GTGGGGCTGCGGCGCCTGGGACTGCACCGCACGGAGGGCGAGCTCCGGAAAATTGTGCAAGCAGGTGACA
  AGGACCTTGATGGGCAACTGGACTTTGAAGAGTTTGTACATTACCTCCAAGATCATGAGAAAAAACTGAG
  GCTGGTGTTCAAGAGTCTGGACAAAAAGAATGATGGTCGAATCGATGCTCAGGAGATCATGCAGTCCCTG
  CGGGACCTGGGTGTCAAGATCTCGGAACAGCAGGCGGAGAAGATTCTTAAGAGAATACGGACGGGCCACT
  TCTGGGGCCCTGTCACCTACATGGATAAGAATGGCACGATGACCATCGACTGGAACGAGTGGAGGGACTA
  CCACCTCCTGCACCCTGTGGAGAACATCCCGGAGATCATCCTGTACTGGAAGCACTCGACGATCTTCGAT
  GTCGGTGAGAATCTGACAGTCCCAGATGAGTTCACAGTGGAGGAGAGGCAGACGGGGATGTGGTGGAGGC
  ACCTGGTGGCAGGAGGTGGGGCAGGGGCAGTTTCCAGAACCTGCACTGCCCCCCTGGACAGACTGAAGGT
  GCTCATGCAGGTCCATGCCTCCCGCAGCAACAACATGTGCATCGTAGGTGGATTCACACAGATGATTCGA
  GAAGGGGGAGCCAAGTCACTCTGGCGGGGCAACGGCATCAATGTCCTCAAAATTGCCCCTGAGTCGGCCA
  TCAAATTCATGGCATATGAGCAGATGAAACGGCTTGTCGGTAGTGATCAGGAGACGCTGAGGATCCACGA
  AAGGCTTGTGGCAGGCTCCTTGGCCGGAGCCATTGCCCAGAGTAGCATCTACCCAATGGAGGTTCTGAAG
  ACCCGAATGGCCCTGCGGAAAACAGGACAGTACTCCGGCATGCTGGACTGTGCCAGGAGGATCTTGGCTA
  AAGAGGGTGTAGCTGCCTTCTACAAAGGCTACATCCCCAACATGCTGGGGATCATCCCCTATGCTGGCAT
  CGACCTAGCTGTCTATGAGACATTGAAAAATACCTGGCTCCAGCGCTACGCAGTAAACAGTGCAGACCCC
  GGTGTGTTCGTGCTCCTGGCCTGTGGTACTATCTCCAGTACTTGTGGCCAGCTGGCCAGCTACCCACTAG
  CCCTGGTCAGGACCCGGATGCAGGCACAAGCCTCCATTGAGGGCGCACCTGAGGTAACCATGAGCAGCCT
  CTTCAAACAGATTCTGCGGACTGAGGGGGCCTTTGGGCTCTACCGGGGGCTGGCCCCCAACTTCATGAAG
  GTGATCCCGGCTGTGAGCATCAGCTACGTGGTCTACGAAAACCTGAAGATCACCCTGGGCGTGCAGTCTC
  GG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208251 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVSSVLCRCVASPPPDAAATASSSASSPASVGDPCGGAVCGGPDHQLRLWSLFQTLDVNRDGGLCVNDLA
  VGLRRLGLHRTEGELRKIVQAGDKDLDGQLDFEEFVHYLQDHEKKLRLVFKSLDKKNDGRIDAQEIMQSL
  RDLGVKISEQQAEKILKRIRTGHFWGPVTYMDKNGTMTIDWNEWRDYHLLHPVENIPEIILYWKHSTIFD
  VGENLTVPDEFTVEERQTGMWWRHLVAGGGAGAVSRTCTAPLDRLKVLMQVHASRSNNMCIVGGFTQMIR
  EGGAKSLWRGNGINVLKIAPESAIKFMAYEQMKRLVGSDQETLRIHERLVAGSLAGAIAQSSIYPMEVLK
  TRMALRKTGQYSGMLDCARRILAKEGVAAFYKGYIPNMLGIIPYAGIDLAVYETLKNTWLQRYAVNSADP
  GVFVLLACGTISSTCGQLASYPLALVRTRMQAQASIEGAPEVTMSSLFKQILRTEGAFGLYRGLAPNFMK
  VIPAVSISYVVYENLKITLGVQSR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_146118
• ORF Size: 1545 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146118.3, NP_666230.2
• RefSeq Size: 3342 bp
• RefSeq ORF: 1545 bp
• Locus ID: 227731
• UniProt ID: A2ASZ8
• Cytogenetics: 2 B
• MW: 56.8 kDa
• Gene Summary: Calcium-dependent mitochondrial solute carrier. Mitochondrial solute carriers shuttle metabolites, nucleotides, and cofactors through the mitochondrial inner membrane. May act as a ATP-Mg/Pi exchanger that mediates the transport of Mg-ATP in exchange for phosphate, catalyzing the net uptake or efflux of adenine nucleotides into or from the mitochondria (By similarity).[UniProtKB/Swiss-Prot Function]