Slc2a4 (NM_009204) Mouse Tagged ORF Clone

CAT#: MR208202

Slc2a4 (Myc-DDK-tagged) - Mouse solute carrier family 2 (facilitated glucose transporter), member 4 (Slc2a4)

Product Images

Circular map for MR208202

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc2a4
• Synonyms: Glut-4; Glut4; GT2; twgy
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208202 representing NM_009204
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGTCGGGTTTCCAGCAGATCGGCTCTGACGATGGGGAACCCCCTCGGCAGCGAGTGACTGGAACAC
  TGGTCCTAGCTGTATTCTCAGCTGTGCTTGGCTCCCTTCAGTTTGGCTATAACATTGGGGTTATCAATGC
  CCCACAGAAGGTGATTGAACAGAGCTACAATGCAACGTGGCTGGGTAGGCAAGGTCCTGGGGGACCGGAT
  TCCATCCCACAAGGCACCCTCACTACGCTCTGGGCTCTCTCCGTGGCCATCTTCTCTGTGGGTGGCATGA
  TCTCTTCCTTTCTCATTGGCATCATTTCTCAATGGTTGGGAAGGAAAAGGGCTATGCTGGCCAACAATGT
  CTTGGCCGTGTTGGGGGGCGCCCTCATGGGCCTAGCCAATGCCGCGGCCTCCTATGAGATACTCATTCTT
  GGACGGTTCCTCATTGGCGCCTACTCAGGGCTAACATCAGGGCTGGTGCCCATGTATGTGGGAGAAATCG
  CCCCCACTCATCTTCGGGGTGCCTTGGGAACACTCAACCAACTGGCCATCGTCATTGGCATTCTGGTTGC
  CCAGGTGCTGGGCTTGGAGTCTATGCTGGGCACAGCTACCCTGTGGCCACTGCTTCTGGCTCTCACAGTA
  CTCCCTGCTCTCCTGCAGCTGATTCTGCTGCCCTTCTGTCCTGAGAGCCCCAGATACCTCTACATCATCC
  GGAACCTGGAGGGGCCTGCCCGAAAGAGTCTAAAGCGCCTGACCGGCTGGGCTGATGTGTCTGACGCACT
  AGCTGAGCTGAAGGATGAGAAACGGAAGTTGGAGAGAGAGCGTCCAATGTCCTTGCTCCAGCTCCTGGGC
  AGCCGCACCCACCGGCAGCCTCTGATCATCGCAGTGGTGCTGCAGCTGAGCCAACAGCTCTCAGGCATCA
  ATGCTGTTTTCTACTATTCAACCAGCATCTTCGAGTCGGCTGGGGTGGGACAGCCAGCCTACGCCACCAT
  AGGAGCTGGTGTGGTCAATACGGTCTTCACGTTGGTCTCGGTGCTCTTAGTAGAACGAGCTGGACGACGG
  ACACTCCATCTGTTGGGCCTGGCCGGCATGTGTGGCTGTGCCATCTTGATGACCGTGGCTCTGCTGCTGC
  TGGAACGGGTTCCAGCCATGAGCTATGTCTCCATCGTGGCCATATTTGGCTTTGTGGCCTTCTTTGAGAT
  TGGCCCTGGCCCCATTCCCTGGTTCATTGTGGCAGAGCTCTTCAGCCAGGGCCCCCGCCCAGCCGCCATG
  GCTGTCGCTGGTTTCTCCAACTGGACCTGTAACTTCATTGTCGGCATGGGTTTCCAGTATGTTGCGGATG
  CTATGGGTCCTTACGTCTTCCTTCTATTTGCCGTCCTCCTGCTTGGCTTCTTCATCTTCACCTTCCTAAA
  AGTGCCTGAAACCAGAGGCCGGACGTTTGACCAGATCTCAGCTGCCTTCCGACGGACACCTTCCCTTTTA
  GAGCAGGAGGTGAAACCCAGTACAGAACTTGAATACTTAGGGCCAGATGAGAATGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208202 representing NM_009204
  Red=Cloning site Green=Tags(s)
  
  MPSGFQQIGSDDGEPPRQRVTGTLVLAVFSAVLGSLQFGYNIGVINAPQKVIEQSYNATWLGRQGPGGPD
  SIPQGTLTTLWALSVAIFSVGGMISSFLIGIISQWLGRKRAMLANNVLAVLGGALMGLANAAASYEILIL
  GRFLIGAYSGLTSGLVPMYVGEIAPTHLRGALGTLNQLAIVIGILVAQVLGLESMLGTATLWPLLLALTV
  LPALLQLILLPFCPESPRYLYIIRNLEGPARKSLKRLTGWADVSDALAELKDEKRKLERERPMSLLQLLG
  SRTHRQPLIIAVVLQLSQQLSGINAVFYYSTSIFESAGVGQPAYATIGAGVVNTVFTLVSVLLVERAGRR
  TLHLLGLAGMCGCAILMTVALLLLERVPAMSYVSIVAIFGFVAFFEIGPGPIPWFIVAELFSQGPRPAAM
  AVAGFSNWTCNFIVGMGFQYVADAMGPYVFLLFAVLLLGFFIFTFLKVPETRGRTFDQISAAFRRTPSLL
  EQEVKPSTELEYLGPDEND
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009204
• ORF Size: 1527 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009204.2, NP_033230.2
• RefSeq Size: 2822 bp
• RefSeq ORF: 1530 bp
• Locus ID: 20528
• UniProt ID: P14142
• Cytogenetics: 11 42.93 cM
• MW: 55.2 kDa
• Gene Summary: Insulin-regulated facilitative glucose transporter, which plays a key role in removal of glucose from circulation (PubMed:26240143, PubMed:26629404). Response to insulin is regulated by its intracellular localization: in the absence of insulin, it is efficiently retained intracellularly within storage compartments in muscle and fat cells (PubMed:26240143, PubMed:26629404). Upon insulin stimulation, translocates from these compartments to the cell surface where it transports glucose from the extracellular milieu into the cell (PubMed:26240143, PubMed:26629404).[UniProtKB/Swiss-Prot Function]