Slc2a4 (NM_009204) Mouse Tagged ORF Clone

CAT#: MR208202

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  • TrueORF®

Slc2a4 (Myc-DDK-tagged) - Mouse solute carrier family 2 (facilitated glucose transporter), member 4 (Slc2a4)



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Reconstitution Protocol

USD 474.00

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Size
    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Slc2a4
Synonyms Glut-4; Glut4; GT2; twgy
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR208202 representing NM_009204
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGTCGGGTTTCCAGCAGATCGGCTCTGACGATGGGGAACCCCCTCGGCAGCGAGTGACTGGAACAC
TGGTCCTAGCTGTATTCTCAGCTGTGCTTGGCTCCCTTCAGTTTGGCTATAACATTGGGGTTATCAATGC
CCCACAGAAGGTGATTGAACAGAGCTACAATGCAACGTGGCTGGGTAGGCAAGGTCCTGGGGGACCGGAT
TCCATCCCACAAGGCACCCTCACTACGCTCTGGGCTCTCTCCGTGGCCATCTTCTCTGTGGGTGGCATGA
TCTCTTCCTTTCTCATTGGCATCATTTCTCAATGGTTGGGAAGGAAAAGGGCTATGCTGGCCAACAATGT
CTTGGCCGTGTTGGGGGGCGCCCTCATGGGCCTAGCCAATGCCGCGGCCTCCTATGAGATACTCATTCTT
GGACGGTTCCTCATTGGCGCCTACTCAGGGCTAACATCAGGGCTGGTGCCCATGTATGTGGGAGAAATCG
CCCCCACTCATCTTCGGGGTGCCTTGGGAACACTCAACCAACTGGCCATCGTCATTGGCATTCTGGTTGC
CCAGGTGCTGGGCTTGGAGTCTATGCTGGGCACAGCTACCCTGTGGCCACTGCTTCTGGCTCTCACAGTA
CTCCCTGCTCTCCTGCAGCTGATTCTGCTGCCCTTCTGTCCTGAGAGCCCCAGATACCTCTACATCATCC
GGAACCTGGAGGGGCCTGCCCGAAAGAGTCTAAAGCGCCTGACCGGCTGGGCTGATGTGTCTGACGCACT
AGCTGAGCTGAAGGATGAGAAACGGAAGTTGGAGAGAGAGCGTCCAATGTCCTTGCTCCAGCTCCTGGGC
AGCCGCACCCACCGGCAGCCTCTGATCATCGCAGTGGTGCTGCAGCTGAGCCAACAGCTCTCAGGCATCA
ATGCTGTTTTCTACTATTCAACCAGCATCTTCGAGTCGGCTGGGGTGGGACAGCCAGCCTACGCCACCAT
AGGAGCTGGTGTGGTCAATACGGTCTTCACGTTGGTCTCGGTGCTCTTAGTAGAACGAGCTGGACGACGG
ACACTCCATCTGTTGGGCCTGGCCGGCATGTGTGGCTGTGCCATCTTGATGACCGTGGCTCTGCTGCTGC
TGGAACGGGTTCCAGCCATGAGCTATGTCTCCATCGTGGCCATATTTGGCTTTGTGGCCTTCTTTGAGAT
TGGCCCTGGCCCCATTCCCTGGTTCATTGTGGCAGAGCTCTTCAGCCAGGGCCCCCGCCCAGCCGCCATG
GCTGTCGCTGGTTTCTCCAACTGGACCTGTAACTTCATTGTCGGCATGGGTTTCCAGTATGTTGCGGATG
CTATGGGTCCTTACGTCTTCCTTCTATTTGCCGTCCTCCTGCTTGGCTTCTTCATCTTCACCTTCCTAAA
AGTGCCTGAAACCAGAGGCCGGACGTTTGACCAGATCTCAGCTGCCTTCCGACGGACACCTTCCCTTTTA
GAGCAGGAGGTGAAACCCAGTACAGAACTTGAATACTTAGGGCCAGATGAGAATGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR208202 representing NM_009204
Red=Cloning site Green=Tags(s)

MPSGFQQIGSDDGEPPRQRVTGTLVLAVFSAVLGSLQFGYNIGVINAPQKVIEQSYNATWLGRQGPGGPD
SIPQGTLTTLWALSVAIFSVGGMISSFLIGIISQWLGRKRAMLANNVLAVLGGALMGLANAAASYEILIL
GRFLIGAYSGLTSGLVPMYVGEIAPTHLRGALGTLNQLAIVIGILVAQVLGLESMLGTATLWPLLLALTV
LPALLQLILLPFCPESPRYLYIIRNLEGPARKSLKRLTGWADVSDALAELKDEKRKLERERPMSLLQLLG
SRTHRQPLIIAVVLQLSQQLSGINAVFYYSTSIFESAGVGQPAYATIGAGVVNTVFTLVSVLLVERAGRR
TLHLLGLAGMCGCAILMTVALLLLERVPAMSYVSIVAIFGFVAFFEIGPGPIPWFIVAELFSQGPRPAAM
AVAGFSNWTCNFIVGMGFQYVADAMGPYVFLLFAVLLLGFFIFTFLKVPETRGRTFDQISAAFRRTPSLL
EQEVKPSTELEYLGPDEND

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_009204
ORF Size 1527 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_009204.2, NP_033230.2
RefSeq Size 2822 bp
RefSeq ORF 1530 bp
Locus ID 20528
UniProt ID P14142
Cytogenetics 11 42.93 cM
MW 55.2 kDa
Gene Summary Insulin-regulated facilitative glucose transporter, which plays a key role in removal of glucose from circulation (PubMed:26240143, PubMed:26629404). Response to insulin is regulated by its intracellular localization: in the absence of insulin, it is efficiently retained intracellularly within storage compartments in muscle and fat cells (PubMed:26240143, PubMed:26629404). Upon insulin stimulation, translocates from these compartments to the cell surface where it transports glucose from the extracellular milieu into the cell (PubMed:26240143, PubMed:26629404).[UniProtKB/Swiss-Prot Function]
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