Camk2g (NM_001039139) Mouse Tagged ORF Clone

CAT#: MR207945

Camk2g (Myc-DDK-tagged) - Mouse calcium/calmodulin-dependent protein kinase II gamma (Camk2g), transcript variant 3

Product Images

Circular map for MR207945

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Camk2g
• Synonyms: Camkg
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207945 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACCACCGCCACCTGCACCCGCTTCACCGACGACTACCAGCTTTTCGAGGAGCTCGGCAAGGGTG
  CCTTCTCAGTGGTCCGGAGGTGTGTAAAGAAAACATCTACGCAGGAATATGCTGCAAAAATCATCAATAC
  CAAGAAGTTGTCCGCCCGAGATCATCAGAAACTAGAACGAGAGGCCCGGATATGCCGACTTCTGAAACAT
  CCAAACATTGTGCGTCTCCATGACAGTATTTCTGAAGAAGGGTTTCACTACCTCGTGTTTGACCTTGTTA
  CCGGAGGGGAGTTGTTTGAAGACATTGTGGCCAGAGAGTACTACAGTGAAGCTGATGCCAGCCACTGTAT
  ACATCAGATCCTGGAGAGTGTTAACCACATCCACCAGCATGACATCGTCCACCGGGATCTGAAGCCTGAG
  AACTTGCTGCTGGCGAGTAAATGCAAGGGTGCTGCGGTCAAGCTAGCTGATTTTGGCCTGGCCATCGAAG
  TACAGGGAGAGCAGCAGGCTTGGTTTGGTTTTGCCGGCACCCCAGGTTACTTGTCCCCTGAGGTCTTGAG
  GAAAGATCCCTATGGAAAACCTGTGGACATCTGGGCCTGCGGGGTCATCCTGTATATCCTCCTGGTGGGC
  TACCCTCCCTTCTGGGATGAGGATCAGCACAAGCTGTATCAGCAGATCAAAGCTGGAGCCTACGATTTCC
  CATCACCAGAATGGGACACAGTCACTCCTGAAGCTAAGAACTTGATCAACCAGATGCTGACCATAAACCC
  TGCAAAGCGCATCACGGCCGACCAGGCTCTCAAGCACCCATGGGTCTGTCAACGGTCTACGGTGGCATCC
  ATGATGCATCGCCAAGAGACGGTAGAGTGCTTACGCAAATTCAACGCCCGGAGAAAACTGAAGGGTGCCA
  TCCTCACAACCATGCTTGTCTCCAGGAACTTTTCAGCTGCCAAAAGCCTATTGAACAAGAAGTCAGATGG
  CGGTGTCAAGGAACCACAAACCACCGTGGTACATAATGCTACAGATGGGATCAAGGGCTCCACAGAGAGC
  TGTAACACCACTACAGAAGACGAAGATCTCAAAGTGAGAAAACAAGAAATCATTAAGATCACAGAACAAC
  TGATCGAAGCCATCAACAATGGGGACTTTGAGGCCTACACGAAGATTTGTGACCCGGGCCTCACATCCTT
  TGAGCCAGAAGCCCTTGGTAACCTCGTGGAAGGAATGGATTTCCATAAGTTTTACTTTGAGAATCTCCTG
  TCCAAGAACAGCAAGCCTATCCACACCACCATCCTAAACCCTCACGTCCACGTGATTGGGGAGGACGCAG
  CTTGCATCGCCTATATCCGGCTCACTCAGTACATCGACGGGCAGGGTCGGCCTCGCACCAGTCAGTCAGA
  GGAGACCCGGGTGTGGCACCGCCGGGATGGCAAGTGGCTCAATGTCCACTATCACTGCTCAGGGGCCCCT
  GCCGCACCGCTACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207945 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATTATCTRFTDDYQLFEELGKGAFSVVRRCVKKTSTQEYAAKIINTKKLSARDHQKLEREARICRLLKH
  PNIVRLHDSISEEGFHYLVFDLVTGGELFEDIVAREYYSEADASHCIHQILESVNHIHQHDIVHRDLKPE
  NLLLASKCKGAAVKLADFGLAIEVQGEQQAWFGFAGTPGYLSPEVLRKDPYGKPVDIWACGVILYILLVG
  YPPFWDEDQHKLYQQIKAGAYDFPSPEWDTVTPEAKNLINQMLTINPAKRITADQALKHPWVCQRSTVAS
  MMHRQETVECLRKFNARRKLKGAILTTMLVSRNFSAAKSLLNKKSDGGVKEPQTTVVHNATDGIKGSTES
  CNTTTEDEDLKVRKQEIIKITEQLIEAINNGDFEAYTKICDPGLTSFEPEALGNLVEGMDFHKFYFENLL
  SKNSKPIHTTILNPHVHVIGEDAACIAYIRLTQYIDGQGRPRTSQSEETRVWHRRDGKWLNVHYHCSGAP
  AAPLQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001039139
• ORF Size: 1488 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039139.2
• RefSeq Size: 3624 bp
• RefSeq ORF: 1488 bp
• Locus ID: 12325
• UniProt ID: Q923T9
• Cytogenetics: 14 A3
• MW: 56 kDa
• Gene Summary: Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in sarcoplasmic reticulum Ca(2+) transport in skeletal muscle and may function in dendritic spine and synapse formation and neuronal plasticity. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of the ryanodine receptor-coupling factor triadin. In neurons, may participate in the promotion of dendritic spine and synapse formation and maintenance of synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning (By similarity).[UniProtKB/Swiss-Prot Function]