Nfia (BC075702) Mouse Tagged ORF Clone

CAT#: MR207901

Nfia (Myc-DDK-tagged) - Mouse nuclear factor I/A (cDNA clone MGC:76704 IMAGE:30087137)

Product Images

Circular map for MR207901

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nfia
• Synonyms: 1110047K16Rik; 9430022M17Rik; CTF; NF1-A; NF1A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207901 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATTCTCCGCTCTGTCTCACCCAGGATGAGTTTCATCCTTTCATTGAAGCACTTCTGCCCCATGTCC
  GCGCCTTCGCCTACACATGGTTCAACCTGCAGGCCCGAAAGCGGAAATACTTCAAAAAACATGAGAAGCG
  CATGTCGAAAGAAGAGGAGAGGGCCGTGAAGGATGAACTGCTAAGCGAGAAGCCCGAGGTCAAGCAGAAG
  TGGGCTTCCCGACTTCTGGCCAAGTTACGGAAAGATATCCGACCCGAGTACCGAGAGGATTTTGTTCTTA
  CAGTTACAGGGAAAAAACCTCCATGCTGTGTTCTTTCCAACCCTGATCAGAAAGGCAAGATGCGGAGAAT
  TGACTGCCTCCGCCAGGCAGATAAAGTATGGAGGTTGGACCTCGTCATGGTGATCTTGTTCAAAGGTATT
  CCGCTGGAAAGTACTGATGGCGAGCGCCTTGTAAAGAGTCCACAGTGCTCTAATCCAGGGCTCTGTGTCC
  AGCCCCATCACATAGGGGTTTCTGTAAAGGAACTCGATTTATATTTGGCATACTTTGTACATGCAGCAGA
  TTCAAGTCAATCTGAAAGTCCCAGCCAGCCAAGTGAAGCTGACATTAAGGACCAGCCAGAAAATGGACAT
  TTGGGCTTCCAGGACAGCTTTGTCACATCAGGTGTTTTCAGTGTGACTGAGCTAGTAAGAGTGTCACAAA
  CACCAATAGCTGCAGGAACCGGCCCCAATTTTTCTCTCTCTGATTTGGAAAGTTCTTCATACTACAGCAT
  GAGTCCAGGAGCAATGAGGAGGTCTCTGCCCAGCACATCCTCTACCAGCTCTACAAAGCGCCTCAAGTCT
  GTGGAGGATGAAATGGACAGTCCTGGTGAAGAACCATTTTACACAGGCCAAGGGCGCTCCCCAGGGAGTG
  GCAGCCAGTCCAGTGGATGGCATGAAGTAGAGCCAGGCTTGCCATCTCCAAGCACACTGAAGAAGTCTGA
  GAAGTCTGGTTTCAGCAGCCCCTCCCCTTCGCAGACCTCCTCCCTGGGAACAGCATTCACACAGCATCAC
  CGACCTGTCATTACAGGACCCAGAGCAAGTCCACATGCGACGCCATCGACTCTCCACTTTCCAACGTCAC
  CCATCATCCAGCAGCCTGGGCCTTACTTCTCACACCCAGCCATCCGTTACCACCCTCAGGAGACGCTGAA
  AGAGTTTGTCCAACTTGTCTGTCCTGATGCTGGTCAGCAAGCTGGACAGGTGGGGTTCCTCAATCCCAAT
  GGAAGCAGTCAAGGCAAGGTGCACAACCCATTCCTCCCCACCCCAATGTTGCCGCCGCCGCCACCACCAC
  CGATGGCCAGACACCAAGCCTCCAACCACACCTCACCGACCTACTCGACACCCAGCACCTCCCCCGCAAA
  CCGATTCGTCAGTGTTGGACCACGGGATCCAAGCTTTGTAAATATCCCTCAACAGACACAGTCCTGGTAC
  CTGGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207901 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MYSPLCLTQDEFHPFIEALLPHVRAFAYTWFNLQARKRKYFKKHEKRMSKEEERAVKDELLSEKPEVKQK
  WASRLLAKLRKDIRPEYREDFVLTVTGKKPPCCVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKGI
  PLESTDGERLVKSPQCSNPGLCVQPHHIGVSVKELDLYLAYFVHAADSSQSESPSQPSEADIKDQPENGH
  LGFQDSFVTSGVFSVTELVRVSQTPIAAGTGPNFSLSDLESSSYYSMSPGAMRRSLPSTSSTSSTKRLKS
  VEDEMDSPGEEPFYTGQGRSPGSGSQSSGWHEVEPGLPSPSTLKKSEKSGFSSPSPSQTSSLGTAFTQHH
  RPVITGPRASPHATPSTLHFPTSPIIQQPGPYFSHPAIRYHPQETLKEFVQLVCPDAGQQAGQVGFLNPN
  GSSQGKVHNPFLPTPMLPPPPPPPMARHQASNHTSPTYSTPSTSPANRFVSVGPRDPSFVNIPQQTQSWY
  LG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC075702
• ORF Size: 1476 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC075702, AAH75702
• RefSeq Size: 3001 bp
• RefSeq ORF: 1478 bp
• Locus ID: 18027
• Cytogenetics: 4 45.52 cM
• MW: 54.4 kDa
• Gene Summary: Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.[UniProtKB/Swiss-Prot Function]