C730004C24Rik (BC092260) Mouse Tagged ORF Clone

CAT#: MR207861

C730004C24Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA C730004C24 gene (cDNA clone MGC:102487 IMAGE:5132591)

Product Images

Circular map for MR207861

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C730004C24Rik
• Synonyms: C730004C24Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207861 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCCATTTGTAGTCTTGGTGCTTTGTCTGTCCTTTCTGCTTGTCCTGTCATTGTGGAGACAGAGAT
  CTGCAAGAGGGAACCTCCCTCCTGGCCCCACTCCTCTCCCAATTATTGGCAATTACCACCTGATAGATAT
  GAAGGACATTGGCCAGTGCCTTACCAATTTTTCTAAAACATATGGCCCTGTGTTCACTCTGTATTTTGGC
  TCACAGCCTATTGTAGTGTTACATGGTTATGAGGCAATGAAAGAAGCCTTCATTGACCATGGAGAAGAGT
  TCTCTGGAAGAGGAAGGTTTCCGTTTTTTGACAAGGTTACTAAAGGAAAGGGCATTGGTTTTAGCCATGG
  AAATGTATGGAAAGCCACAAGGGTCTTCACAATCAATACCTTGAGGAACTTGGGCATGGGAAAAAGGACC
  ATTGAGAACAAAGTGCAAGAGGAAGCACAGTGGCTCATGAAGGAACTGAAGAAAACAAATGGCTTACCCT
  GTGATCCCCAATTCATCATAGGATGTGCTCCCTGCAATGTCATCTGCTCCATTGTTTTCCAGAATCGTTT
  TGATTATAAGGATAAGGATTTTCTTAGCTTGATAGGAAAAGTGAATGAATGCACTGAAATTTTGAGCTCT
  CCTGGGTGTCAGATTTTCAATGCAGTCCCTATTCTTATTGATTATTGTCCAGGAAGGCATAATAAATTTT
  TTAAAAATCATACATGGATTAAGAGCTATCTTTTGGAGAAAATAAAAGAACATGAAGAATCATTGGATGT
  TACAAATCCTCGGGACTTCATTGATTACTTCCTAATTCAAAGATGTCAGGAAAAAGGCATTGAGCACATG
  GAATATACCATTGAACACCTGGCAACATTGGTGACTGATCTGGTTTTTGGTGGGACAGAGTCCTTAAGCT
  CAACAATGAGATTTGCTCTCCTGCTCTTGATGAAGCACACACACATCACAGCTAAAGTCCAGGAAGAGAT
  TGACAATGTGATTGGGAGACACCGCAGCCCCTGTATGCAGGACAGGAACCATATGCCTTACACAAATGCC
  ATGGTGCATGAGGTCCAACGATATGTTGACCTTGGCCCAATTAGTTTGGTTCATGAAGTAACCTGTGACA
  CTAAGTTCAGAAACTACTTCATCCCCAAGGGAACACAGGTAATGACATCACTGACATCAGTGCTGCATGA
  CAGCACGGAGTTCCCCAACCCAGAGGTGTTTGACCCTGGCCACTTTCTAGATGACAATGGAAACTTTAAG
  AAAAGTGACTACTTCGTGCCTTTCTCAGCAGGAAAACGGATTTGTGTGGGAGAGAGCCTTGCCCGCATGG
  AGCTGTTTCTATTCCTGACCACCATTTTACAGAATTTCAAGCTGAAACCTCTGGTTGATCCAAAGGACAT
  CGACATGACCCCCAAACATTCTGGATTTTCTAAAATTCCTCCCAATTTCCAGATGTGCTTTATCCCTGTG
  GAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207861 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDPFVVLVLCLSFLLVLSLWRQRSARGNLPPGPTPLPIIGNYHLIDMKDIGQCLTNFSKTYGPVFTLYFG
  SQPIVVLHGYEAMKEAFIDHGEEFSGRGRFPFFDKVTKGKGIGFSHGNVWKATRVFTINTLRNLGMGKRT
  IENKVQEEAQWLMKELKKTNGLPCDPQFIIGCAPCNVICSIVFQNRFDYKDKDFLSLIGKVNECTEILSS
  PGCQIFNAVPILIDYCPGRHNKFFKNHTWIKSYLLEKIKEHEESLDVTNPRDFIDYFLIQRCQEKGIEHM
  EYTIEHLATLVTDLVFGGTESLSSTMRFALLLLMKHTHITAKVQEEIDNVIGRHRSPCMQDRNHMPYTNA
  MVHEVQRYVDLGPISLVHEVTCDTKFRNYFIPKGTQVMTSLTSVLHDSTEFPNPEVFDPGHFLDDNGNFK
  KSDYFVPFSAGKRICVGESLARMELFLFLTTILQNFKLKPLVDPKDIDMTPKHSGFSKIPPNFQMCFIPV
  E
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC092260
• ORF Size: 1473 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC092260, AAH92260
• RefSeq Size: 1719 bp
• RefSeq ORF: 1475 bp
• Locus ID: 545288
• Cytogenetics: 19 C3
• MW: 56.1 kDa