Aldh8a1 (NM_178713) Mouse Tagged ORF Clone

CAT#: MR207802

Aldh8a1 (Myc-DDK-tagged) - Mouse aldehyde dehydrogenase 8 family, member A1 (Aldh8a1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aldh8a1
• Synonyms: Raldh4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207802 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGAAAAAGGGAACTTTTGATGTTGGAGAACTTTATAGGTGGGAAGTTTTTACCCTGTAACTCAT
  ATATAGATTCTTACGATCCATCAACAGGGGAAGTTTATTGCAAAGTTCCAAACAGTGGGAAAGAAGAGAT
  TGAAGCTGCCGTGGAGGCTGCCAGAGAGGCCTTCCCTGCCTGGTCATCCCGGAGTCCCCAGGAACGTTCT
  CTCGTCCTGAACAGGCTGGCTGATGTACTGGAGCAGTCTCTGGAGGAGTTAGCTCAGGCAGAATCTAAAG
  ACCAAGGGAAAACCCTCACGCTGGCAAGGACCATGGACATTCCTCGGTCTGTTCTGAACTTCCGGTTCTT
  CGCTTCCTCCAACCTGCACCACGTATCGGAGTGCACACAGATGAGCCACCTGGGCTGTATGCATTACACC
  GTTCGCACCCCGGTGGGAATTGCTGGTTTGATCAGCCCTTGGAATTTACCACTCTACCTGCTGACCTGGA
  AGATAGCTCCGGCCATCGCTGCTGGGAATACCGTGATAGCCAAGCCCAGTGAGATGACTTCTGTGACTGC
  CTGGATGTTTTGTAAACTCTTGGATAAAGCAGGTGTCCCACCAGGTGTGATCAACATTGTGTTTGGAACA
  GGGCCGAGGGTTGGTGAAGCCCTTGTGTCTCACCCCGAGGTACCCTTGATCTCGTTCACTGGGAGCCAGC
  CCACAGCTGAACGGATTACCCAGCTCAGTGCCCCCCACTGCAAGAAGCTTTCCCTGGAGCTGGGAGGCAA
  GAATCCTGCTATCATCTTTGAGGATGCCAACCTGGAGGAGTGTATCCCGGCAACCGTTAGGTCCAGCTTT
  GCTAACCAGGGGGAGATCTGCCTTTGTACCAGTAGAATCTTTGTCCAGAGAAGCATCTACAGTGAATTTC
  TGAAGAGGTTTGTGGAAGCTACCAGAAAGTGGAAAGTTGGTGTTCCTTCTGACCCATCAGCCAACATGGG
  TGCTCTGATAAGCAAAGCACATTTGGAGAAAGTCAGAAGTTATGTCTTGAAAGCTCAGACTGAGGGTGCC
  CGGATTCTCTGTGGTGAGGGTGTGGACCAGCTGAGCCTTCCACTCAGGAACCAGGCAGGCTACTTCATGC
  TTCCCACGGTGATAACAGACATTAAGGATGAGTCCCGCTGCATGACGGAAGAGATCTTTGGTCCAGTGAC
  GTGTGTTGTTCCGTTTGATAGTGAAGAGGAAGTGATTACGAGAGCTAACAGTGTTAGATACGGGCTGGCA
  GCCACAGTGTGGTCCAAGGATGTGGGGCGCATCCACAGGGTAGCTAAGAAGCTTCAGTCGGGGCTGGTCT
  GGACCAACTGCTGGCTCATCAGGGAGCTGAACCTGCCCTTTGGAGGGATGAAGAGCTCTGGAATAGGAAG
  AGAAGGAGCCAAGGACTCATATGACTTCTTTACTGAAATAAAAACCATTACCATTAAATAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207802 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGKRELLMLENFIGGKFLPCNSYIDSYDPSTGEVYCKVPNSGKEEIEAAVEAAREAFPAWSSRSPQERS
  LVLNRLADVLEQSLEELAQAESKDQGKTLTLARTMDIPRSVLNFRFFASSNLHHVSECTQMSHLGCMHYT
  VRTPVGIAGLISPWNLPLYLLTWKIAPAIAAGNTVIAKPSEMTSVTAWMFCKLLDKAGVPPGVINIVFGT
  GPRVGEALVSHPEVPLISFTGSQPTAERITQLSAPHCKKLSLELGGKNPAIIFEDANLEECIPATVRSSF
  ANQGEICLCTSRIFVQRSIYSEFLKRFVEATRKWKVGVPSDPSANMGALISKAHLEKVRSYVLKAQTEGA
  RILCGEGVDQLSLPLRNQAGYFMLPTVITDIKDESRCMTEEIFGPVTCVVPFDSEEEVITRANSVRYGLA
  ATVWSKDVGRIHRVAKKLQSGLVWTNCWLIRELNLPFGGMKSSGIGREGAKDSYDFFTEIKTITIKY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178713
• ORF Size: 1464 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178713.3
• RefSeq Size: 2243 bp
• RefSeq ORF: 1464 bp
• Locus ID: 237320
• UniProt ID: Q8BH00
• Cytogenetics: 10 A3
• MW: 53.7 kDa
• Gene Summary: Catalyzes the NAD-dependent oxidation of 2-aminomuconic semialdehyde of the kynurenine metabolic pathway in L-tryptophan degradation.[UniProtKB/Swiss-Prot Function]