Chst3 (NM_016803) Mouse Tagged ORF Clone

CAT#: MR207541

Chst3 (Myc-DDK-tagged) - Mouse carbohydrate (chondroitin 6/keratan) sulfotransferase 3 (Chst3)

Product Images

Circular map for MR207541

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Chst3
• Synonyms: C6ST; C6ST-1; GST-0
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207541 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAAGGACTCGCTTTGCCTCAGGATTTCCGGGACCTTGTACACAGCCTAAAGATTCGAGGCAGAT
  ACGTCTTGTTCCTGGCATTTGTGGTCATAGTTTTTATCTTCATTGAAAAGGAAAATAAAATCATATCCAG
  GGTCTCCGACAAGCTGAAGCAGATCCCTCATTTTGTGGCAGATGCCAACAGCACTGACCCAGCCCTGCTC
  TTATCGGAGAATGCATCTCTCTTGTCCCTGAGCGAGTTGGATTCCACCTTTTCCCATCTGCGGAGCCGCC
  TGCACAACCTGAGCCTGCAGCTGGGCGTGGAGCCAGCAATGGAGAGCCAGGAGGCTGGGGCAGAGAAGCC
  ATCCCAGCAGGCTGGAGCAGGGACCCGGCGCCACGTGCTTCTCATGGCCACCACCCGCACGGGTTCCTCG
  TTCGTGGGCGAGTTCTTCAACCAGCAGGGCAATATCTTCTACCTCTTCGAGCCACTGTGGCACATCGAGC
  GCACCGTGTTCTTCCAGCAGCGAGGCGCCAGCGCGGCTGGTTCAGCCTTGGTCTACCGTGATGTCCTCAA
  GCAGTTGTTGCTATGCGACCTGTATGTGCTGGAGCCCTTCATCAGCCCTCCGCCCGAGGACCACTTGACT
  CAGTTCCTGTTCCGCCGGGGATCCAGCCGTTCACTCTGCGAGGATCCGGTGTGCACACCCTTCGTCAAGA
  AGGTCTTTGAGAAGTACCACTGCAGGAACCGTCGCTGCGGGCCACTCAACGTGACCTTGGCGGGCGAGGC
  CTGCCGCCGCAAGGACCACGTGGCCCTCAAGGCTGTGCGCATCCGTCAGCTGGAGTTCCTGCAGCCGCTA
  GTTGAGGACCCGAGGTTGGATCTACGAGTCATTCAGCTGGTGCGCGACCCCCGGGCCGTGCTGGCTTCAC
  GCATAGTGGCCTTTGCGGGCAAGTATGAGAACTGGAAGAAGTGGCTGTCCGAGGGGCAGGACCAGCTGAG
  CGAGGATGAGGTGCAGCGATTGCGGGGCAACTGTGAGAGCATCCGCCTGTCTGCAGAGCTGGGCTTGCGG
  CAGCCAGCCTGGCTGCGCGGTCGTTACATGCTGGTGCGCTATGAGGATGTGGCACGCAGGCCACTGCAGA
  AGGCCCGAGAGATGTACAGCTTTGCGGGCATCCCCTTGACCCCGCAGGTGGAGGACTGGATCCAGAAGAA
  CACGCAGGCGACACGCGACAGCAGCGATGTCTACTCCACTCAGAAAAACTCTTCTGAGCAGTTTGAGAAG
  TGGCGCTTCAGCATGCCTTTCAAGCTGGCACAGGTGGTACAGGCTGCCTGTGGCCCGACCATGCACCTCT
  TTGGCTACAAGTTGGCCAGGGATGCCGCCTCACTCACCAACCGCTCCATCAGCCTGCTGGAGGAGCGGGG
  CACCTTCTGGGTCACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207541 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEKGLALPQDFRDLVHSLKIRGRYVLFLAFVVIVFIFIEKENKIISRVSDKLKQIPHFVADANSTDPALL
  LSENASLLSLSELDSTFSHLRSRLHNLSLQLGVEPAMESQEAGAEKPSQQAGAGTRRHVLLMATTRTGSS
  FVGEFFNQQGNIFYLFEPLWHIERTVFFQQRGASAAGSALVYRDVLKQLLLCDLYVLEPFISPPPEDHLT
  QFLFRRGSSRSLCEDPVCTPFVKKVFEKYHCRNRRCGPLNVTLAGEACRRKDHVALKAVRIRQLEFLQPL
  VEDPRLDLRVIQLVRDPRAVLASRIVAFAGKYENWKKWLSEGQDQLSEDEVQRLRGNCESIRLSAELGLR
  QPAWLRGRYMLVRYEDVARRPLQKAREMYSFAGIPLTPQVEDWIQKNTQATRDSSDVYSTQKNSSEQFEK
  WRFSMPFKLAQVVQAACGPTMHLFGYKLARDAASLTNRSISLLEERGTFWVT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016803
• ORF Size: 1419 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016803.2, NP_058083.1
• RefSeq Size: 6129 bp
• RefSeq ORF: 1437 bp
• Locus ID: 53374
• UniProt ID: O88199
• Cytogenetics: 10 B4
• MW: 54 kDa
• Gene Summary: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Can also sulfate Gal residues of keratan sulfate, another glycosaminoglycan, and the Gal residues in sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides. May play a role in the maintenance of naive T-lymphocytes in the spleen.[UniProtKB/Swiss-Prot Function]