Rora (BC003757) Mouse Tagged ORF Clone

CAT#: MR207347

Rora (Myc-DDK-tagged) - Mouse RAR-related orphan receptor alpha (cDNA clone MGC:5892 IMAGE:3592667)

Product Images

Circular map for MR207347

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rora
• Synonyms: ROR1, ROR2, ROR3, Nr1f1, staggerer
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207347 representing BC003757
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGCTCAAATTGAAATTATTCCATGCAAGATCTGTGGAGACAAATCGTCAGGAATCCATTATGGTG
  TCATTACGTGTGAAGGCTGCAAGGGCTTTTTCAGGAGAAGTCAGCAGAGCAATGCCACCTACTCCTGTCC
  TCGTCAGAAGAACTGTTTGATTGATCGGACCAGCAGAAACCGCTGCCAGCATTGTCGGCTGCAGAAATGC
  CTGGCCGTGGGGATGTCTCGAGATGCTGTCAAGTTTGGTCGGATGTCCAAGAAGCAGAGAGACAGCTTGT
  ACGCCGAGGTACAGAAGCACCGGATGCAGCAGCAGCAGCGAGACCACCAGCAGCAGCCTGGGGAGGCGGA
  GCCGCTGACGCCCACCTACAACATCTCAGCCAATGGGCTGACGGAACTGCATGATGACCTCAGCACCTAT
  ATGGATGGGCACACCCCCGAGGGCAGCAAGGCCGACTCAGCCGTCAGCAGCTTCTACCTGGACATCCAGC
  CCTCCCCAGACCAGTCGGGATTGGACATCAATGGGATCAAACCCGAACCCATATGTGACTACACACCAGC
  ATCTGGCTTCTTCCCCTACTGTTCCTTCACCAACGGAGAGACTTCCCCAACCGTGTCCATGGCAGAACTA
  GAACACCTTGCCCAGAACATATCCAAATCCCACCTGGAAACCTGCCAGTACTTGCGGGAAGAGCTCCAGC
  AGATAACGTGGCAGACCTTCCTGCAGGAGGAGATTGAAAACTACCAGAACAAGCAGAGAGAGGTGATGTG
  GCAGCTGTGTGCCATCAAGATTACAGAAGCTATCCAGTATGTGGTGGAGTTTGCCAAACGCATTGATGGA
  TTTATGGAGCTGTGTCAAAATGATCAAATTGTGCTTCTAAAAGCAGGCTCGCTAGAGGTGGTGTTTATTA
  GGATGTGCCGTGCCTTTGACTCTCAGAACAACACCGTGTACTTTGACGGGAAGTATGCGAGCCCCGATGT
  CTTCAAGTCCCTAGGTTGTGAAGACTTCATCAGCTTTGTGTTTGAATTTGGGAAGAGTTTGTGTTCTATG
  CACCTGACCGAAGACGAAATCGCGTTATTTTCTGCATTCGTACTGATGTCAGCGGATCGCTCGTGGCTTC
  AGGAAAAGGTAAAAATAGAAAAGCTGCAACAGAAAATTCAGCTGGCCCTTCAGCACGTCCTACAGAAGAA
  CCACCGAGAAGATGGAATTCTAACCAAGCTAATATGCAAGGTGTCTACGTTAAGAGCCCTATGTGGACGA
  CATACGGAAAAGCTAATGGCATTTAAAGCAATATACCCAGACATTGTGCGACTCCATTTTCCTCCATTAT
  ACAAGGAATTGTTCACTTCAGAATTTGAGCCAGCCATGCAAATCGATGGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207347 representing BC003757
  Red=Cloning site Green=Tags(s)
  
  MKAQIEIIPCKICGDKSSGIHYGVITCEGCKGFFRRSQQSNATYSCPRQKNCLIDRTSRNRCQHCRLQKC
  LAVGMSRDAVKFGRMSKKQRDSLYAEVQKHRMQQQQRDHQQQPGEAEPLTPTYNISANGLTELHDDLSTY
  MDGHTPEGSKADSAVSSFYLDIQPSPDQSGLDINGIKPEPICDYTPASGFFPYCSFTNGETSPTVSMAEL
  EHLAQNISKSHLETCQYLREELQQITWQTFLQEEIENYQNKQREVMWQLCAIKITEAIQYVVEFAKRIDG
  FMELCQNDQIVLLKAGSLEVVFIRMCRAFDSQNNTVYFDGKYASPDVFKSLGCEDFISFVFEFGKSLCSM
  HLTEDEIALFSAFVLMSADRSWLQEKVKIEKLQQKIQLALQHVLQKNHREDGILTKLICKVSTLRALCGR
  HTEKLMAFKAIYPDIVRLHFPPLYKELFTSEFEPAMQIDG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC003757
• ORF Size: 1380 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC003757, AAH03757
• RefSeq Size: 2381 bp
• RefSeq ORF: 1382 bp
• Locus ID: 19883
• Cytogenetics: 9 37.45 cM
• MW: 87.2 kDa
• Gene Summary: The protein encoded by this gene is a member of the NR1 subfamily of nuclear hormone receptors. It can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The encoded protein has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, as well as with NM23-1, the product of a tumor metastasis suppressor candidate gene. Also, it has been shown to aid in the transcriptional regulation of some genes involved in circadian rhythm. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2014]