Shq1 (NM_181590) Mouse Tagged ORF Clone

CAT#: MR207315

Shq1 (Myc-DDK-tagged) - Mouse SHQ1 homolog (S. cerevisiae) (Shq1)

Product Images

Circular map for MR207315

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Shq1
• Synonyms: 2810403P18Rik; Grim-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207315 representing NM_181590
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTACTCCTGCGTTCGAGCTGACCCAGGACTGCGACTTCCTCACCGTCGCCATCCGCGTGCCCCACG
  CGCGCGCCTCTGAGTTCGACGTGTACTTCGAGGGGGTGGACTTCAAGTTCTACGCCAAGCCGTACTTTCT
  GAGATTAACTCTGCCTGGAAGGATTGTGGAAAATGGATCTGAGCAGGGGACCTATGATGCAGATAAAGGA
  ATTTTTACCATTCGATTGCCCAAGGAAACTCCTGGCCAACATTTTGAGGGGCTGAACATGTTAACTGCTC
  TTCTGGCCCCGAGAAAATCCAGGTCTGCCAAGCCACTTGTGGAAGAAATAGGTGCTTCCGGGGTTGCTGA
  GGAAGGAGCTGACGACGAGGATGAAGAGTTTGACTGGGAAATCGAGCAGACTCCCTATGAAGAGGTCTCA
  GAAAGTACTCTGCAATCACAGTGTCACTATGGATTTGGAAACCTGCGGGCCGGTGTGGTTCAGCGGTTAC
  AGGATGAGCTGAGTGAGGTTATCGATATTAAGGATCCAGATTTCACTCCTGTGACTGAACGGAGGCAGAA
  GCGCCTGGCTGCTGAGTTGGCCAAATTTGATCCCGATCATTATCTAGCTGACTTCTTTGAAGACGAGGCA
  GTTGAGCAGATTTTGAAGTACAGTCCTTGGTGGAATGATGCACATGCAGAAATGGTGGCCTCTTTGGGAA
  AGAACCAAGAGCAAGGGGACTCTGCTGCATTAGTGTCTTTTTCAGAAGAGGAAAAATATCAGCTACGAAA
  ATTTGTGAATAAGTCTTACCTGCTAGACAAGACGGCTCACCGTCAAGTGTACTATGGTTTGGTTGACATC
  CTGCTGGCCTATTGCTATGAAGTACGTGTCACTGAAGGAGAGCACAGCGTTGAATCTGCGTGGACCATCA
  GAAAGCTGAGTCCAACACTCTGCTGGTTTGAGACCTGGACAGACGTTCATGAAATCCTGGTGTCCTTTGG
  AAGGAGAGTTTTGTGCTATCCACTTTATCGTCATTTCAAGCTGGTGCTGAAAGCCTACCGAGACACTATA
  AAGATACTGCAACTAGGTAAGAGTGCAGTTTTAAAGTGTCTCCTGGATGTTCACAAAGTCTTTCAGGAAA
  ATGACCCAGCATATATTCTGAATGATCTCTACATCTCAGACTACTGTGTATGGATTCAGAAGGCCAAGTC
  CAAGAAGTTGGCTGCCCTAACCGAAGCCTTGAAAGCAGTCTCGCTCAGTAAGGCCCAGCTGGGGTTAGAG
  TTGCCAGAACTGGAAGCCGCCGCACTCCTCGTCCAGGAGGAGGAAGGTGCTGGAAGAGCAGCCCAGTGCA
  ATCCCTCGCATCAGGCTCCACACTCCAGTCCCGAGCCCAGTGACTCGGACAGCACCTCATCCTCTGGCAC
  AGAGGACTCAGCGTCAGAGCAAGAGGAGCCTGCTGGGACCCAGCCCTCCCTGCTCACTTCTCTGCAAGCC
  CCACTTTTGGAAGAGGACAGTGCCCTGATCATCAGGGAGAGTGGGCTCTGCAGAAACATGGCCAGCCAGG
  GATGTGAAGTCAGTCAAGGACAGCCACTGGCCTCCGCCCGGGCTCCTCTCATAGAGGAGCTGGGAGATGC
  GCTGAAGATGCTTCGGGTTTCCACACCTCACGGTGTCAGTGCTGGGAGCCACGGGAGTGTAGAGGATGCA
  GAGGAGAGGGGTCAGGTGCCCTGCGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207315 representing NM_181590
  Red=Cloning site Green=Tags(s)
  
  MLTPAFELTQDCDFLTVAIRVPHARASEFDVYFEGVDFKFYAKPYFLRLTLPGRIVENGSEQGTYDADKG
  IFTIRLPKETPGQHFEGLNMLTALLAPRKSRSAKPLVEEIGASGVAEEGADDEDEEFDWEIEQTPYEEVS
  ESTLQSQCHYGFGNLRAGVVQRLQDELSEVIDIKDPDFTPVTERRQKRLAAELAKFDPDHYLADFFEDEA
  VEQILKYSPWWNDAHAEMVASLGKNQEQGDSAALVSFSEEEKYQLRKFVNKSYLLDKTAHRQVYYGLVDI
  LLAYCYEVRVTEGEHSVESAWTIRKLSPTLCWFETWTDVHEILVSFGRRVLCYPLYRHFKLVLKAYRDTI
  KILQLGKSAVLKCLLDVHKVFQENDPAYILNDLYISDYCVWIQKAKSKKLAALTEALKAVSLSKAQLGLE
  LPELEAAALLVQEEEGAGRAAQCNPSHQAPHSSPEPSDSDSTSSSGTEDSASEQEEPAGTQPSLLTSLQA
  PLLEEDSALIIRESGLCRNMASQGCEVSQGQPLASARAPLIEELGDALKMLRVSTPHGVSAGSHGSVEDA
  EERGQVPCD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181590
• ORF Size: 1707 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181590.5
• RefSeq Size: 1990 bp
• RefSeq ORF: 1710 bp
• Locus ID: 72171
• UniProt ID: Q7TMX5
• Cytogenetics: 6 D3
• MW: 63.5 kDa
• Gene Summary: Required for the quantitative accumulation of H/ACA ribonucleoproteins (RNPs), including telomerase, probably through the stabilization of DKC1, from the time of its synthesis until its association with NOP10, NHP2, and NAF1 at the nascent H/ACA RNA.[UniProtKB/Swiss-Prot Function]