Idh2 (NM_173011) Mouse Tagged ORF Clone

CAT#: MR207208

Idh2 (Myc-DDK-tagged) - Mouse isocitrate dehydrogenase 2 (NADP+), mitochondrial (Idh2), nuclear gene encoding mitochondrial protein

Product Images

Circular map for MR207208

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Idh2
• Synonyms: E430004F23; Idh-2; IDPm
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207208 representing NM_173011
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGCTACCTGCGGGCTGTGAGCTCGCTCTGCAGAGCCTCGGGCTCAGCGCGGACCTGGGCACCGG
  CAGCACTGACTGTCCCCAGCTGGCCGGAGCAGCCGCGGCGCCACTATGCTGAGAAGAGGATCAAGGTGGA
  GAAGCCGGTAGTGGAGATGGACGGTGACGAGATGACCCGGATCATCTGGCAGTTCATCAAGGAGAAGCTC
  ATCCTGCCTCACGTGGATGTTCAGCTCAAGTATTTTGACCTTGGGCTTCCAAACCGTGACCAGACCAATG
  ACCAGGTCACCATTGACTCTGCTCTGGCCACCCAGAAGTACAGTGTGGCTGTCAAGTGTGCCACAATCAC
  CCCTGATGAGGCCCGTGTGGAAGAGTTCAAGCTGAAGAAAATGTGGAAGAGCCCTAACGGAACGATCCGG
  AACATCCTTGGGGGAACCGTCTTCAGAGAGCCAATCATCTGCAAAAACATCCCCCGCCTTGTCCCTGGCT
  GGACCAAGCCCATCACCATTGGCAGGCACGCCCATGGCGACCAGTACAAGGCCACAGATTTTGTGGTAGA
  TCGAGCTGGCACGTTCAAGTTGGTCTTCACCCCAAAGGATGGCAGCAGTGCCAAGGAGTGGGAGGTGTAT
  AACTTCCCTGCCGGAGGCGTTGGGATGGGGATGTACAACACCGACGAGTCCATTTCGGGCTTCGCGCACA
  GCTGCTTCCAGTACTCTATCCAGAAGAAATGGCCGCTCTACTTGAGCACCAAGAACACCATTCTGAAGGC
  CTATGACGGGCGTTTCAAGGACATCTTCCAGGAGATCTTTGACAAGCACTATAAGACTGACTTCGACAAG
  AATAAGATCTGGTATGAACATCGGCTCATCGACGACATGGTGGCCCAGGTGCTCAAGTCTTCCGGTGGCT
  TTGTGTGGGCTTGCAAAAACTATGATGGAGACGTGCAGTCTGACATCCTGGCTCAAGGCTTTGGCTCCCT
  CGGCCTGATGACATCTGTGCTGGTCTGCCCTGATGGGAAGACAATTGAGGCTGAGGCTGCTCATGGGACA
  GTCACCCGCCATTACCGAGAACACCAGAAGGGCCGGCCCACCAGTACCAACCCTATTGCCAGCATCTTTG
  CCTGGACACGGGGTCTGGAGCATCGTGGGAAGCTGGATGGGAACCAGGACCTTATCAGGTTTGCACAGAC
  GCTGGAGAAGGTGTGCGTGCAGACTGTGGAGAGCGGAGCGATGACCAAGGACCTGGCTGGCTGTATCCAT
  GGCCTCAGCAATGTGAAGCTGAACGAGCACTTCCTGAACACCACAGACTTCCTGGACACCATTAAGAGCA
  ACCTGGACAGAGCTCTGGGCAAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207208 representing NM_173011
  Red=Cloning site Green=Tags(s)
  
  MAGYLRAVSSLCRASGSARTWAPAALTVPSWPEQPRRHYAEKRIKVEKPVVEMDGDEMTRIIWQFIKEKL
  ILPHVDVQLKYFDLGLPNRDQTNDQVTIDSALATQKYSVAVKCATITPDEARVEEFKLKKMWKSPNGTIR
  NILGGTVFREPIICKNIPRLVPGWTKPITIGRHAHGDQYKATDFVVDRAGTFKLVFTPKDGSSAKEWEVY
  NFPAGGVGMGMYNTDESISGFAHSCFQYSIQKKWPLYLSTKNTILKAYDGRFKDIFQEIFDKHYKTDFDK
  NKIWYEHRLIDDMVAQVLKSSGGFVWACKNYDGDVQSDILAQGFGSLGLMTSVLVCPDGKTIEAEAAHGT
  VTRHYREHQKGRPTSTNPIASIFAWTRGLEHRGKLDGNQDLIRFAQTLEKVCVQTVESGAMTKDLAGCIH
  GLSNVKLNEHFLNTTDFLDTIKSNLDRALGKQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173011
• ORF Size: 1356 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173011.2, NP_766599.2
• RefSeq Size: 1718 bp
• RefSeq ORF: 1359 bp
• Locus ID: 269951
• UniProt ID: P54071
• Cytogenetics: 7 45.43 cM
• MW: 51.4 kDa
• Gene Summary: Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.[UniProtKB/Swiss-Prot Function]